Strain Fitness in Escherichia coli ECOR27 around NOLOHH_19000

Experiment: Control_ECOR27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntatoD and NOLOHH_18995 are separated by 2 nucleotidesNOLOHH_18995 and NOLOHH_19000 are separated by 9 nucleotidesNOLOHH_19000 and NOLOHH_19005 overlap by 11 nucleotidesNOLOHH_19005 and gntT are separated by 45 nucleotides NOLOHH_18990: atoD - succinyl-CoA--3-ketoacid-CoA transferase, at 3,732,311 to 3,733,018 atoD NOLOHH_18995: NOLOHH_18995 - succinyl-CoA--3-ketoacid-CoA transferase, at 3,733,021 to 3,733,677 _18995 NOLOHH_19000: NOLOHH_19000 - succinyl-CoA--3-ketoacid-CoA transferase, at 3,733,687 to 3,734,892 _19000 NOLOHH_19005: NOLOHH_19005 - 3-hydroxybutyryl-CoA dehydrogenase, at 3,734,882 to 3,735,805 _19005 NOLOHH_19010: gntT - transporter, at 3,735,851 to 3,737,242 gntT Position (kb) 3733 3734 3735Strain fitness (log2 ratio) -1 0 1 2at 3732.740 kb on + strand, within atoDat 3732.741 kb on - strand, within atoDat 3733.075 kb on + strandat 3733.120 kb on + strand, within NOLOHH_18995at 3733.218 kb on + strand, within NOLOHH_18995at 3733.946 kb on + strand, within NOLOHH_19000at 3734.012 kb on + strand, within NOLOHH_19000at 3734.012 kb on + strand, within NOLOHH_19000at 3734.013 kb on - strand, within NOLOHH_19000at 3734.013 kb on - strand, within NOLOHH_19000at 3734.013 kb on - strand, within NOLOHH_19000at 3734.740 kb on - strand, within NOLOHH_19000at 3735.183 kb on - strand, within NOLOHH_19005at 3735.619 kb on + strand, within NOLOHH_19005at 3735.672 kb on + strand, within NOLOHH_19005at 3735.673 kb on - strand, within NOLOHH_19005at 3735.756 kb on - strandat 3735.827 kb on + strandat 3735.828 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR27
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3,732,740 + atoD NOLOHH_18990 0.61 +0.4
3,732,741 - atoD NOLOHH_18990 0.61 +0.8
3,733,075 + +1.0
3,733,120 + NOLOHH_18995 0.15 +0.0
3,733,218 + NOLOHH_18995 0.30 +0.2
3,733,946 + NOLOHH_19000 0.21 -0.2
3,734,012 + NOLOHH_19000 0.27 -0.5
3,734,012 + NOLOHH_19000 0.27 -1.5
3,734,013 - NOLOHH_19000 0.27 +0.9
3,734,013 - NOLOHH_19000 0.27 -1.6
3,734,013 - NOLOHH_19000 0.27 +1.6
3,734,740 - NOLOHH_19000 0.87 +0.3
3,735,183 - NOLOHH_19005 0.33 +0.4
3,735,619 + NOLOHH_19005 0.80 -0.6
3,735,672 + NOLOHH_19005 0.85 -1.3
3,735,673 - NOLOHH_19005 0.86 +1.2
3,735,756 - +0.2
3,735,827 + +2.2
3,735,828 - +0.2

Or see this region's nucleotide sequence