Experiment: Control_ECOR27
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tqsA and mdtJ are separated by 411 nucleotides mdtJ and mdtI overlap by 14 nucleotides mdtI and ydgD are separated by 38 nucleotides ydgD and ydgU are separated by 99 nucleotides
NOLOHH_18205: tqsA - AI-2 transporter TqsA, at 3,578,094 to 3,579,128
tqsA
NOLOHH_18210: mdtJ - multidrug/spermidine efflux SMR transporter subunit MdtJ, at 3,579,540 to 3,579,905
mdtJ
NOLOHH_18215: mdtI - multidrug/spermidine efflux SMR transporter subunit MdtI, at 3,579,892 to 3,580,221
mdtI
NOLOHH_18220: ydgD - Uncharacterized serine protease YdgD, at 3,580,260 to 3,581,081
ydgD
NOLOHH_18225: ydgU - Uncharacterized protein YdgU, at 3,581,181 to 3,581,264
ydgU
Position (kb)
3579
3580
3581 Strain fitness (log2 ratio)
-1
0
1
2 at 3579.063 kb on - strand at 3579.869 kb on + strand at 3579.998 kb on + strand, within mdtI at 3580.262 kb on - strand at 3580.626 kb on - strand, within ydgD at 3580.875 kb on - strand, within ydgD at 3581.076 kb on - strand at 3581.102 kb on + strand at 3581.109 kb on - strand at 3581.109 kb on - strand at 3581.112 kb on - strand at 3581.127 kb on + strand at 3581.131 kb on + strand at 3581.131 kb on + strand at 3581.132 kb on - strand at 3581.135 kb on - strand at 3581.176 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR27 remove 3,579,063 - -0.3 3,579,869 + +1.1 3,579,998 + mdtI NOLOHH_18215 0.32 -1.6 3,580,262 - -0.2 3,580,626 - ydgD NOLOHH_18220 0.45 -1.7 3,580,875 - ydgD NOLOHH_18220 0.75 -0.2 3,581,076 - -0.3 3,581,102 + +1.8 3,581,109 - +1.1 3,581,109 - +0.5 3,581,112 - +0.3 3,581,127 + +0.7 3,581,131 + -0.6 3,581,131 + +0.3 3,581,132 - -0.5 3,581,135 - -0.5 3,581,176 + +1.1
Or see this region's nucleotide sequence