Experiment: Control_ECOR27
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fumB and fumC are separated by 141 nucleotides fumC and tus overlap by 4 nucleotides
NOLOHH_18150: fumB - class I fumarate hydratase, at 3,564,665 to 3,566,311
fumB
NOLOHH_18155: fumC - class II fumarate hydratase, at 3,566,453 to 3,567,856
fumC
NOLOHH_18160: tus - DNA replication terminus site-binding protein, at 3,567,853 to 3,568,782
tus
Position (kb)
3566
3567
3568 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3565.633 kb on + strand, within fumB at 3565.684 kb on + strand, within fumB at 3565.697 kb on + strand, within fumB at 3565.697 kb on + strand, within fumB at 3565.697 kb on + strand, within fumB at 3565.804 kb on + strand, within fumB at 3565.847 kb on - strand, within fumB at 3565.901 kb on + strand, within fumB at 3565.901 kb on + strand, within fumB at 3566.003 kb on + strand, within fumB at 3566.105 kb on + strand, within fumB at 3566.136 kb on + strand, within fumB at 3566.136 kb on + strand, within fumB at 3566.155 kb on + strand at 3566.277 kb on + strand at 3566.277 kb on + strand at 3566.458 kb on + strand at 3567.094 kb on + strand, within fumC at 3567.159 kb on + strand, within fumC at 3567.838 kb on + strand at 3567.945 kb on - strand at 3567.985 kb on - strand, within tus at 3568.255 kb on - strand, within tus at 3568.255 kb on - strand, within tus at 3568.280 kb on - strand, within tus at 3568.280 kb on - strand, within tus at 3568.405 kb on - strand, within tus at 3568.444 kb on - strand, within tus at 3568.618 kb on - strand, within tus at 3568.783 kb on - strand at 3568.818 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR27 remove 3,565,633 + fumB NOLOHH_18150 0.59 -0.6 3,565,684 + fumB NOLOHH_18150 0.62 +1.2 3,565,697 + fumB NOLOHH_18150 0.63 -0.8 3,565,697 + fumB NOLOHH_18150 0.63 +1.7 3,565,697 + fumB NOLOHH_18150 0.63 +0.7 3,565,804 + fumB NOLOHH_18150 0.69 -0.1 3,565,847 - fumB NOLOHH_18150 0.72 +0.8 3,565,901 + fumB NOLOHH_18150 0.75 +0.2 3,565,901 + fumB NOLOHH_18150 0.75 -0.0 3,566,003 + fumB NOLOHH_18150 0.81 -0.1 3,566,105 + fumB NOLOHH_18150 0.87 -0.0 3,566,136 + fumB NOLOHH_18150 0.89 +0.3 3,566,136 + fumB NOLOHH_18150 0.89 -1.0 3,566,155 + +0.9 3,566,277 + +0.1 3,566,277 + +0.2 3,566,458 + +0.3 3,567,094 + fumC NOLOHH_18155 0.46 -0.0 3,567,159 + fumC NOLOHH_18155 0.50 -3.1 3,567,838 + -2.3 3,567,945 - -0.0 3,567,985 - tus NOLOHH_18160 0.14 +0.6 3,568,255 - tus NOLOHH_18160 0.43 +1.3 3,568,255 - tus NOLOHH_18160 0.43 +0.4 3,568,280 - tus NOLOHH_18160 0.46 -1.4 3,568,280 - tus NOLOHH_18160 0.46 +1.4 3,568,405 - tus NOLOHH_18160 0.59 +2.0 3,568,444 - tus NOLOHH_18160 0.64 -2.0 3,568,618 - tus NOLOHH_18160 0.82 -0.5 3,568,783 - +2.1 3,568,818 - +0.1
Or see this region's nucleotide sequence