Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potE and pgm are separated by 55 nucleotides pgm and seqA are separated by 25 nucleotides seqA and ybfF are separated by 184 nucleotides
NOLOHH_23475: potE - putrescine-ornithine antiporter, at 4,622,803 to 4,624,122
potE
NOLOHH_23480: pgm - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 4,624,178 to 4,625,818
pgm
NOLOHH_23485: seqA - replication initiation negative regulator SeqA, at 4,625,844 to 4,626,389
seqA
NOLOHH_23490: ybfF - esterase, at 4,626,574 to 4,627,338
ybfF
Position (kb)
4624
4625
4626 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4623.287 kb on + strand, within potE at 4623.377 kb on + strand, within potE at 4623.465 kb on + strand, within potE at 4623.492 kb on + strand, within potE at 4623.492 kb on + strand, within potE at 4623.514 kb on + strand, within potE at 4623.514 kb on + strand, within potE at 4623.892 kb on + strand, within potE at 4623.892 kb on + strand, within potE at 4623.894 kb on + strand, within potE at 4623.894 kb on + strand, within potE at 4623.914 kb on + strand, within potE at 4623.914 kb on + strand, within potE at 4623.921 kb on + strand, within potE at 4623.994 kb on + strand at 4623.994 kb on + strand at 4624.075 kb on + strand at 4624.075 kb on + strand at 4624.076 kb on - strand at 4624.273 kb on + strand at 4624.274 kb on - strand at 4624.276 kb on - strand at 4624.276 kb on - strand at 4624.276 kb on - strand at 4624.276 kb on - strand at 4624.486 kb on - strand, within pgm at 4624.580 kb on - strand, within pgm at 4624.934 kb on - strand, within pgm at 4624.934 kb on - strand, within pgm at 4625.068 kb on - strand, within pgm at 4625.249 kb on - strand, within pgm at 4625.249 kb on - strand, within pgm at 4625.575 kb on - strand, within pgm at 4625.575 kb on - strand, within pgm at 4625.575 kb on - strand, within pgm at 4625.736 kb on - strand at 4625.739 kb on + strand at 4625.740 kb on - strand at 4625.740 kb on - strand at 4625.740 kb on - strand at 4625.743 kb on - strand at 4625.994 kb on - strand, within seqA at 4626.032 kb on - strand, within seqA at 4626.095 kb on - strand, within seqA at 4626.097 kb on - strand, within seqA at 4626.336 kb on - strand at 4626.336 kb on - strand at 4626.353 kb on - strand at 4626.411 kb on - strand at 4626.419 kb on - strand at 4626.435 kb on - strand at 4626.435 kb on - strand at 4626.437 kb on - strand at 4626.437 kb on - strand at 4626.437 kb on - strand at 4626.443 kb on - strand at 4626.500 kb on + strand at 4626.501 kb on - strand at 4626.501 kb on - strand at 4626.501 kb on - strand at 4626.501 kb on - strand at 4626.501 kb on - strand at 4626.534 kb on + strand at 4626.534 kb on + strand at 4626.534 kb on + strand at 4626.534 kb on + strand at 4626.534 kb on + strand at 4626.534 kb on + strand at 4626.534 kb on + strand at 4626.535 kb on - strand at 4626.535 kb on - strand at 4626.538 kb on - strand at 4626.618 kb on + strand at 4626.618 kb on + strand at 4626.619 kb on - strand at 4626.619 kb on - strand at 4626.726 kb on + strand, within ybfF at 4626.727 kb on - strand, within ybfF at 4626.763 kb on + strand, within ybfF at 4626.792 kb on + strand, within ybfF at 4626.793 kb on - strand, within ybfF
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 4,623,287 + potE NOLOHH_23475 0.37 -0.9 4,623,377 + potE NOLOHH_23475 0.43 +0.1 4,623,465 + potE NOLOHH_23475 0.50 +0.6 4,623,492 + potE NOLOHH_23475 0.52 +0.5 4,623,492 + potE NOLOHH_23475 0.52 +0.1 4,623,514 + potE NOLOHH_23475 0.54 -1.3 4,623,514 + potE NOLOHH_23475 0.54 +2.7 4,623,892 + potE NOLOHH_23475 0.82 +0.6 4,623,892 + potE NOLOHH_23475 0.82 +0.4 4,623,894 + potE NOLOHH_23475 0.83 -0.4 4,623,894 + potE NOLOHH_23475 0.83 +0.4 4,623,914 + potE NOLOHH_23475 0.84 -0.6 4,623,914 + potE NOLOHH_23475 0.84 -1.1 4,623,921 + potE NOLOHH_23475 0.85 +0.5 4,623,994 + -0.5 4,623,994 + +0.6 4,624,075 + -0.6 4,624,075 + +2.5 4,624,076 - -1.9 4,624,273 + -2.1 4,624,274 - -1.6 4,624,276 - -3.0 4,624,276 - -2.6 4,624,276 - -0.9 4,624,276 - -1.5 4,624,486 - pgm NOLOHH_23480 0.19 -3.6 4,624,580 - pgm NOLOHH_23480 0.24 -2.7 4,624,934 - pgm NOLOHH_23480 0.46 +0.5 4,624,934 - pgm NOLOHH_23480 0.46 -3.7 4,625,068 - pgm NOLOHH_23480 0.54 -0.9 4,625,249 - pgm NOLOHH_23480 0.65 -2.5 4,625,249 - pgm NOLOHH_23480 0.65 -2.6 4,625,575 - pgm NOLOHH_23480 0.85 -1.0 4,625,575 - pgm NOLOHH_23480 0.85 -4.0 4,625,575 - pgm NOLOHH_23480 0.85 -0.2 4,625,736 - -3.0 4,625,739 + +0.5 4,625,740 - -1.3 4,625,740 - -3.4 4,625,740 - -2.5 4,625,743 - -2.0 4,625,994 - seqA NOLOHH_23485 0.27 -3.3 4,626,032 - seqA NOLOHH_23485 0.34 +0.5 4,626,095 - seqA NOLOHH_23485 0.46 +0.7 4,626,097 - seqA NOLOHH_23485 0.46 -0.1 4,626,336 - -0.5 4,626,336 - -0.9 4,626,353 - -0.7 4,626,411 - +0.2 4,626,419 - -0.3 4,626,435 - -0.5 4,626,435 - +0.1 4,626,437 - +0.2 4,626,437 - +0.0 4,626,437 - +0.2 4,626,443 - -0.4 4,626,500 + -1.1 4,626,501 - +0.3 4,626,501 - +0.1 4,626,501 - -1.1 4,626,501 - +0.2 4,626,501 - +0.2 4,626,534 + +0.6 4,626,534 + -0.4 4,626,534 + +0.3 4,626,534 + -0.2 4,626,534 + -0.0 4,626,534 + +0.4 4,626,534 + +1.6 4,626,535 - +0.4 4,626,535 - +0.1 4,626,538 - -0.2 4,626,618 + -1.1 4,626,618 + +0.4 4,626,619 - -0.0 4,626,619 - -0.1 4,626,726 + ybfF NOLOHH_23490 0.20 -1.0 4,626,727 - ybfF NOLOHH_23490 0.20 -0.4 4,626,763 + ybfF NOLOHH_23490 0.25 -1.6 4,626,792 + ybfF NOLOHH_23490 0.28 -0.6 4,626,793 - ybfF NOLOHH_23490 0.29 +0.4
Or see this region's nucleotide sequence