Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgaA and pgaB are separated by 8 nucleotides pgaB and pgaC overlap by 8 nucleotides
NOLOHH_21445: pgaA - poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA, at 4,211,405 to 4,213,828
pgaA
NOLOHH_21450: pgaB - poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB, at 4,213,837 to 4,215,855
pgaB
NOLOHH_21455: pgaC - poly-beta-1,6-N-acetyl-D-glucosamine synthase, at 4,215,848 to 4,217,173
pgaC
Position (kb)
4213
4214
4215
4216 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4212.876 kb on + strand, within pgaA at 4212.922 kb on - strand, within pgaA at 4212.945 kb on + strand, within pgaA at 4212.949 kb on + strand, within pgaA at 4212.949 kb on + strand, within pgaA at 4213.043 kb on + strand, within pgaA at 4213.245 kb on - strand, within pgaA at 4213.610 kb on + strand at 4213.618 kb on + strand at 4213.749 kb on + strand at 4213.769 kb on + strand at 4213.769 kb on + strand at 4213.794 kb on + strand at 4213.794 kb on + strand at 4213.794 kb on + strand at 4213.880 kb on + strand at 4213.880 kb on + strand at 4213.881 kb on - strand at 4213.923 kb on + strand at 4213.923 kb on + strand at 4213.923 kb on + strand at 4214.035 kb on - strand at 4214.404 kb on + strand, within pgaB at 4214.447 kb on - strand, within pgaB at 4214.451 kb on - strand, within pgaB at 4215.002 kb on + strand, within pgaB at 4215.047 kb on + strand, within pgaB at 4215.737 kb on + strand at 4215.891 kb on + strand at 4216.011 kb on - strand, within pgaC at 4216.067 kb on - strand, within pgaC at 4216.129 kb on - strand, within pgaC at 4216.151 kb on + strand, within pgaC at 4216.168 kb on + strand, within pgaC at 4216.319 kb on + strand, within pgaC at 4216.370 kb on + strand, within pgaC at 4216.621 kb on + strand, within pgaC at 4216.755 kb on + strand, within pgaC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 4,212,876 + pgaA NOLOHH_21445 0.61 +1.3 4,212,922 - pgaA NOLOHH_21445 0.63 +0.1 4,212,945 + pgaA NOLOHH_21445 0.64 -0.2 4,212,949 + pgaA NOLOHH_21445 0.64 +0.1 4,212,949 + pgaA NOLOHH_21445 0.64 +0.6 4,213,043 + pgaA NOLOHH_21445 0.68 +0.9 4,213,245 - pgaA NOLOHH_21445 0.76 -0.3 4,213,610 + +1.9 4,213,618 + +0.8 4,213,749 + -0.1 4,213,769 + -0.2 4,213,769 + -0.3 4,213,794 + -0.2 4,213,794 + +1.0 4,213,794 + +1.2 4,213,880 + -0.8 4,213,880 + -0.6 4,213,881 - -0.4 4,213,923 + +0.3 4,213,923 + -1.2 4,213,923 + -0.4 4,214,035 - -1.5 4,214,404 + pgaB NOLOHH_21450 0.28 -1.0 4,214,447 - pgaB NOLOHH_21450 0.30 -1.8 4,214,451 - pgaB NOLOHH_21450 0.30 +0.2 4,215,002 + pgaB NOLOHH_21450 0.58 +0.3 4,215,047 + pgaB NOLOHH_21450 0.60 -2.3 4,215,737 + -0.9 4,215,891 + +0.4 4,216,011 - pgaC NOLOHH_21455 0.12 +1.0 4,216,067 - pgaC NOLOHH_21455 0.17 +0.5 4,216,129 - pgaC NOLOHH_21455 0.21 +0.3 4,216,151 + pgaC NOLOHH_21455 0.23 +0.7 4,216,168 + pgaC NOLOHH_21455 0.24 -0.0 4,216,319 + pgaC NOLOHH_21455 0.36 +0.6 4,216,370 + pgaC NOLOHH_21455 0.39 +0.1 4,216,621 + pgaC NOLOHH_21455 0.58 +2.3 4,216,755 + pgaC NOLOHH_21455 0.68 -0.2
Or see this region's nucleotide sequence