Strain Fitness in Escherichia coli ECOR27 around NOLOHH_21130

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntplsX and rpmF are separated by 110 nucleotidesrpmF and yceD are separated by 51 nucleotidesyceD and yceF are separated by 159 nucleotidesyceF and rluC are separated by 111 nucleotides NOLOHH_21120: plsX - phosphate acyltransferase PlsX, at 4,155,395 to 4,156,435 plsX NOLOHH_21125: rpmF - 50S ribosomal protein L32, at 4,156,546 to 4,156,719 rpmF NOLOHH_21130: yceD - 23S rRNA accumulation protein YceD, at 4,156,771 to 4,157,292 yceD NOLOHH_21140: yceF - 7-methyl-GTP pyrophosphatase, at 4,157,452 to 4,158,075 yceF NOLOHH_21145: rluC - 23S rRNA pseudouridine(955/2504/2580) synthase RluC, at 4,158,187 to 4,159,146 rluC Position (kb) 4156 4157 4158Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4155.835 kb on - strand, within plsXat 4155.835 kb on - strand, within plsXat 4155.842 kb on - strand, within plsXat 4155.842 kb on - strand, within plsXat 4155.897 kb on - strand, within plsXat 4156.113 kb on - strand, within plsXat 4156.323 kb on - strand, within plsXat 4156.443 kb on - strandat 4156.522 kb on - strandat 4156.522 kb on - strandat 4156.712 kb on - strandat 4156.773 kb on - strandat 4156.773 kb on - strandat 4156.793 kb on - strandat 4156.793 kb on - strandat 4157.031 kb on - strand, within yceDat 4157.031 kb on - strand, within yceDat 4157.031 kb on - strand, within yceDat 4157.053 kb on - strand, within yceDat 4157.217 kb on - strand, within yceDat 4157.217 kb on - strand, within yceDat 4157.217 kb on - strand, within yceDat 4157.217 kb on - strand, within yceDat 4157.364 kb on + strandat 4157.365 kb on - strandat 4157.394 kb on - strandat 4157.443 kb on + strandat 4157.443 kb on + strandat 4157.443 kb on + strandat 4157.449 kb on - strandat 4157.466 kb on + strandat 4157.467 kb on - strandat 4157.467 kb on - strandat 4157.472 kb on - strandat 4157.473 kb on + strandat 4157.473 kb on + strandat 4157.496 kb on + strandat 4157.503 kb on - strandat 4157.682 kb on - strand, within yceFat 4157.685 kb on + strand, within yceFat 4157.686 kb on - strand, within yceFat 4157.700 kb on + strand, within yceFat 4157.702 kb on + strand, within yceFat 4157.702 kb on + strand, within yceFat 4157.703 kb on - strand, within yceFat 4157.784 kb on + strand, within yceFat 4157.797 kb on + strand, within yceFat 4157.797 kb on + strand, within yceFat 4157.797 kb on + strand, within yceFat 4157.797 kb on + strand, within yceFat 4157.798 kb on - strand, within yceFat 4157.817 kb on + strand, within yceFat 4157.817 kb on + strand, within yceFat 4157.837 kb on + strand, within yceFat 4157.838 kb on - strand, within yceFat 4157.902 kb on + strand, within yceFat 4157.902 kb on + strand, within yceFat 4157.902 kb on + strand, within yceFat 4157.902 kb on + strand, within yceFat 4157.902 kb on + strand, within yceFat 4158.041 kb on + strandat 4158.073 kb on + strandat 4158.074 kb on - strandat 4158.096 kb on + strandat 4158.175 kb on + strandat 4158.176 kb on - strandat 4158.176 kb on - strandat 4158.176 kb on - strandat 4158.176 kb on - strandat 4158.176 kb on - strandat 4158.178 kb on - strandat 4158.186 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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4,155,835 - plsX NOLOHH_21120 0.42 +0.8
4,155,835 - plsX NOLOHH_21120 0.42 -0.8
4,155,842 - plsX NOLOHH_21120 0.43 -0.3
4,155,842 - plsX NOLOHH_21120 0.43 +0.9
4,155,897 - plsX NOLOHH_21120 0.48 +0.1
4,156,113 - plsX NOLOHH_21120 0.69 +0.2
4,156,323 - plsX NOLOHH_21120 0.89 -0.0
4,156,443 - -0.3
4,156,522 - -0.7
4,156,522 - -2.3
4,156,712 - +1.0
4,156,773 - -0.8
4,156,773 - -1.4
4,156,793 - -1.5
4,156,793 - -2.0
4,157,031 - yceD NOLOHH_21130 0.50 -3.1
4,157,031 - yceD NOLOHH_21130 0.50 +0.0
4,157,031 - yceD NOLOHH_21130 0.50 -0.3
4,157,053 - yceD NOLOHH_21130 0.54 +3.0
4,157,217 - yceD NOLOHH_21130 0.85 -2.5
4,157,217 - yceD NOLOHH_21130 0.85 +0.6
4,157,217 - yceD NOLOHH_21130 0.85 -1.5
4,157,217 - yceD NOLOHH_21130 0.85 -0.1
4,157,364 + -0.1
4,157,365 - -1.0
4,157,394 - +0.3
4,157,443 + -0.6
4,157,443 + -0.3
4,157,443 + -1.1
4,157,449 - -1.4
4,157,466 + -0.6
4,157,467 - +0.4
4,157,467 - -0.2
4,157,472 - -0.3
4,157,473 + -0.2
4,157,473 + -1.0
4,157,496 + -3.3
4,157,503 - -0.3
4,157,682 - yceF NOLOHH_21140 0.37 -1.0
4,157,685 + yceF NOLOHH_21140 0.37 -0.2
4,157,686 - yceF NOLOHH_21140 0.38 -0.5
4,157,700 + yceF NOLOHH_21140 0.40 -0.1
4,157,702 + yceF NOLOHH_21140 0.40 -0.5
4,157,702 + yceF NOLOHH_21140 0.40 -2.4
4,157,703 - yceF NOLOHH_21140 0.40 -0.3
4,157,784 + yceF NOLOHH_21140 0.53 -0.1
4,157,797 + yceF NOLOHH_21140 0.55 -1.7
4,157,797 + yceF NOLOHH_21140 0.55 +2.0
4,157,797 + yceF NOLOHH_21140 0.55 -0.1
4,157,797 + yceF NOLOHH_21140 0.55 +0.5
4,157,798 - yceF NOLOHH_21140 0.55 -0.3
4,157,817 + yceF NOLOHH_21140 0.58 -0.9
4,157,817 + yceF NOLOHH_21140 0.58 +0.5
4,157,837 + yceF NOLOHH_21140 0.62 +0.7
4,157,838 - yceF NOLOHH_21140 0.62 -1.8
4,157,902 + yceF NOLOHH_21140 0.72 +0.6
4,157,902 + yceF NOLOHH_21140 0.72 -0.0
4,157,902 + yceF NOLOHH_21140 0.72 -0.7
4,157,902 + yceF NOLOHH_21140 0.72 -0.2
4,157,902 + yceF NOLOHH_21140 0.72 -0.0
4,158,041 + -0.4
4,158,073 + -0.2
4,158,074 - +0.2
4,158,096 + -0.8
4,158,175 + -0.5
4,158,176 - +0.1
4,158,176 - +0.2
4,158,176 - -0.1
4,158,176 - +0.2
4,158,176 - +0.3
4,158,178 - +0.1
4,158,186 - -0.3

Or see this region's nucleotide sequence