Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt plsX and rpmF are separated by 110 nucleotides rpmF and yceD are separated by 51 nucleotides yceD and yceF are separated by 159 nucleotides yceF and rluC are separated by 111 nucleotides
NOLOHH_21120: plsX - phosphate acyltransferase PlsX, at 4,155,395 to 4,156,435
plsX
NOLOHH_21125: rpmF - 50S ribosomal protein L32, at 4,156,546 to 4,156,719
rpmF
NOLOHH_21130: yceD - 23S rRNA accumulation protein YceD, at 4,156,771 to 4,157,292
yceD
NOLOHH_21140: yceF - 7-methyl-GTP pyrophosphatase, at 4,157,452 to 4,158,075
yceF
NOLOHH_21145: rluC - 23S rRNA pseudouridine(955/2504/2580) synthase RluC, at 4,158,187 to 4,159,146
rluC
Position (kb)
4156
4157
4158 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4155.835 kb on - strand, within plsX at 4155.835 kb on - strand, within plsX at 4155.842 kb on - strand, within plsX at 4155.842 kb on - strand, within plsX at 4155.897 kb on - strand, within plsX at 4156.113 kb on - strand, within plsX at 4156.323 kb on - strand, within plsX at 4156.443 kb on - strand at 4156.522 kb on - strand at 4156.522 kb on - strand at 4156.712 kb on - strand at 4156.773 kb on - strand at 4156.773 kb on - strand at 4156.793 kb on - strand at 4156.793 kb on - strand at 4157.031 kb on - strand, within yceD at 4157.031 kb on - strand, within yceD at 4157.031 kb on - strand, within yceD at 4157.053 kb on - strand, within yceD at 4157.217 kb on - strand, within yceD at 4157.217 kb on - strand, within yceD at 4157.217 kb on - strand, within yceD at 4157.217 kb on - strand, within yceD at 4157.364 kb on + strand at 4157.365 kb on - strand at 4157.394 kb on - strand at 4157.443 kb on + strand at 4157.443 kb on + strand at 4157.443 kb on + strand at 4157.449 kb on - strand at 4157.466 kb on + strand at 4157.467 kb on - strand at 4157.467 kb on - strand at 4157.472 kb on - strand at 4157.473 kb on + strand at 4157.473 kb on + strand at 4157.496 kb on + strand at 4157.503 kb on - strand at 4157.682 kb on - strand, within yceF at 4157.685 kb on + strand, within yceF at 4157.686 kb on - strand, within yceF at 4157.700 kb on + strand, within yceF at 4157.702 kb on + strand, within yceF at 4157.702 kb on + strand, within yceF at 4157.703 kb on - strand, within yceF at 4157.784 kb on + strand, within yceF at 4157.797 kb on + strand, within yceF at 4157.797 kb on + strand, within yceF at 4157.797 kb on + strand, within yceF at 4157.797 kb on + strand, within yceF at 4157.798 kb on - strand, within yceF at 4157.817 kb on + strand, within yceF at 4157.817 kb on + strand, within yceF at 4157.837 kb on + strand, within yceF at 4157.838 kb on - strand, within yceF at 4157.902 kb on + strand, within yceF at 4157.902 kb on + strand, within yceF at 4157.902 kb on + strand, within yceF at 4157.902 kb on + strand, within yceF at 4157.902 kb on + strand, within yceF at 4158.041 kb on + strand at 4158.073 kb on + strand at 4158.074 kb on - strand at 4158.096 kb on + strand at 4158.175 kb on + strand at 4158.176 kb on - strand at 4158.176 kb on - strand at 4158.176 kb on - strand at 4158.176 kb on - strand at 4158.176 kb on - strand at 4158.178 kb on - strand at 4158.186 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 4,155,835 - plsX NOLOHH_21120 0.42 +0.8 4,155,835 - plsX NOLOHH_21120 0.42 -0.8 4,155,842 - plsX NOLOHH_21120 0.43 -0.3 4,155,842 - plsX NOLOHH_21120 0.43 +0.9 4,155,897 - plsX NOLOHH_21120 0.48 +0.1 4,156,113 - plsX NOLOHH_21120 0.69 +0.2 4,156,323 - plsX NOLOHH_21120 0.89 -0.0 4,156,443 - -0.3 4,156,522 - -0.7 4,156,522 - -2.3 4,156,712 - +1.0 4,156,773 - -0.8 4,156,773 - -1.4 4,156,793 - -1.5 4,156,793 - -2.0 4,157,031 - yceD NOLOHH_21130 0.50 -3.1 4,157,031 - yceD NOLOHH_21130 0.50 +0.0 4,157,031 - yceD NOLOHH_21130 0.50 -0.3 4,157,053 - yceD NOLOHH_21130 0.54 +3.0 4,157,217 - yceD NOLOHH_21130 0.85 -2.5 4,157,217 - yceD NOLOHH_21130 0.85 +0.6 4,157,217 - yceD NOLOHH_21130 0.85 -1.5 4,157,217 - yceD NOLOHH_21130 0.85 -0.1 4,157,364 + -0.1 4,157,365 - -1.0 4,157,394 - +0.3 4,157,443 + -0.6 4,157,443 + -0.3 4,157,443 + -1.1 4,157,449 - -1.4 4,157,466 + -0.6 4,157,467 - +0.4 4,157,467 - -0.2 4,157,472 - -0.3 4,157,473 + -0.2 4,157,473 + -1.0 4,157,496 + -3.3 4,157,503 - -0.3 4,157,682 - yceF NOLOHH_21140 0.37 -1.0 4,157,685 + yceF NOLOHH_21140 0.37 -0.2 4,157,686 - yceF NOLOHH_21140 0.38 -0.5 4,157,700 + yceF NOLOHH_21140 0.40 -0.1 4,157,702 + yceF NOLOHH_21140 0.40 -0.5 4,157,702 + yceF NOLOHH_21140 0.40 -2.4 4,157,703 - yceF NOLOHH_21140 0.40 -0.3 4,157,784 + yceF NOLOHH_21140 0.53 -0.1 4,157,797 + yceF NOLOHH_21140 0.55 -1.7 4,157,797 + yceF NOLOHH_21140 0.55 +2.0 4,157,797 + yceF NOLOHH_21140 0.55 -0.1 4,157,797 + yceF NOLOHH_21140 0.55 +0.5 4,157,798 - yceF NOLOHH_21140 0.55 -0.3 4,157,817 + yceF NOLOHH_21140 0.58 -0.9 4,157,817 + yceF NOLOHH_21140 0.58 +0.5 4,157,837 + yceF NOLOHH_21140 0.62 +0.7 4,157,838 - yceF NOLOHH_21140 0.62 -1.8 4,157,902 + yceF NOLOHH_21140 0.72 +0.6 4,157,902 + yceF NOLOHH_21140 0.72 -0.0 4,157,902 + yceF NOLOHH_21140 0.72 -0.7 4,157,902 + yceF NOLOHH_21140 0.72 -0.2 4,157,902 + yceF NOLOHH_21140 0.72 -0.0 4,158,041 + -0.4 4,158,073 + -0.2 4,158,074 - +0.2 4,158,096 + -0.8 4,158,175 + -0.5 4,158,176 - +0.1 4,158,176 - +0.2 4,158,176 - -0.1 4,158,176 - +0.2 4,158,176 - +0.3 4,158,178 - +0.1 4,158,186 - -0.3
Or see this region's nucleotide sequence