Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NOLOHH_19000 and NOLOHH_19005 overlap by 11 nucleotides NOLOHH_19005 and gntT are separated by 45 nucleotides gntT and NOLOHH_19015 are separated by 11 nucleotides NOLOHH_19015 and osmC are separated by 34 nucleotides
NOLOHH_19000: NOLOHH_19000 - succinyl-CoA--3-ketoacid-CoA transferase, at 3,733,687 to 3,734,892
_19000
NOLOHH_19005: NOLOHH_19005 - 3-hydroxybutyryl-CoA dehydrogenase, at 3,734,882 to 3,735,805
_19005
NOLOHH_19010: gntT - transporter, at 3,735,851 to 3,737,242
gntT
NOLOHH_19015: NOLOHH_19015 - 3-hydroxybutyrate dehydrogenase, at 3,737,254 to 3,738,024
_19015
NOLOHH_19020: osmC - peroxiredoxin OsmC, at 3,738,059 to 3,738,490
osmC
Position (kb)
3735
3736
3737
3738 Strain fitness (log2 ratio)
-2
-1
0
1 at 3735.183 kb on - strand, within NOLOHH_19005 at 3735.619 kb on + strand, within NOLOHH_19005 at 3735.672 kb on + strand, within NOLOHH_19005 at 3735.673 kb on - strand, within NOLOHH_19005 at 3735.756 kb on - strand at 3735.827 kb on + strand at 3735.828 kb on - strand at 3736.418 kb on - strand, within gntT at 3737.068 kb on + strand, within gntT at 3737.240 kb on + strand at 3737.778 kb on + strand, within NOLOHH_19015 at 3737.778 kb on + strand, within NOLOHH_19015 at 3737.822 kb on + strand, within NOLOHH_19015 at 3737.822 kb on + strand, within NOLOHH_19015
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,735,183 - NOLOHH_19005 0.33 -1.9 3,735,619 + NOLOHH_19005 0.80 -1.2 3,735,672 + NOLOHH_19005 0.85 +1.0 3,735,673 - NOLOHH_19005 0.86 -0.1 3,735,756 - -0.9 3,735,827 + +1.5 3,735,828 - -0.3 3,736,418 - gntT NOLOHH_19010 0.41 -0.1 3,737,068 + gntT NOLOHH_19010 0.87 -1.1 3,737,240 + +1.3 3,737,778 + NOLOHH_19015 0.68 +0.6 3,737,778 + NOLOHH_19015 0.68 +0.1 3,737,822 + NOLOHH_19015 0.74 -1.8 3,737,822 + NOLOHH_19015 0.74 -0.1
Or see this region's nucleotide sequence