Strain Fitness in Escherichia coli ECOR27 around NOLOHH_16620

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhA and flhe overlap by 1 nucleotidesflhe and yecT are separated by 119 nucleotidesyecT and yecV are separated by 90 nucleotidesyecV and argS are separated by 41 nucleotides NOLOHH_16610: flhA - flagellar biosynthesis protein FlhA, at 3,265,470 to 3,267,548 flhA NOLOHH_16615: flhe - flagellar protein FlhE, at 3,267,548 to 3,267,940 flhe NOLOHH_16620: yecT - Uncharacterized protein YecT, at 3,268,060 to 3,268,548 yecT NOLOHH_16625: yecV - Protein YecV, at 3,268,639 to 3,268,683 yecV NOLOHH_16630: argS - arginine--tRNA ligase, at 3,268,725 to 3,270,458 argS Position (kb) 3268 3269Strain fitness (log2 ratio) -2 -1 0 1 2at 3267.298 kb on + strand, within flhAat 3267.298 kb on + strand, within flhAat 3267.301 kb on + strand, within flhAat 3267.515 kb on + strandat 3267.515 kb on + strandat 3267.559 kb on - strandat 3267.561 kb on + strandat 3267.623 kb on - strand, within flheat 3268.142 kb on - strand, within yecTat 3268.150 kb on - strand, within yecTat 3268.480 kb on + strand, within yecTat 3268.516 kb on - strandat 3268.655 kb on + strand, within yecVat 3268.661 kb on + strand, within yecVat 3268.661 kb on + strand, within yecVat 3268.661 kb on + strand, within yecVat 3268.661 kb on + strand, within yecV

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,267,298 + flhA NOLOHH_16610 0.88 +0.2
3,267,298 + flhA NOLOHH_16610 0.88 +2.2
3,267,301 + flhA NOLOHH_16610 0.88 -0.2
3,267,515 + -2.6
3,267,515 + -1.3
3,267,559 - -1.2
3,267,561 + +0.7
3,267,623 - flhe NOLOHH_16615 0.19 +0.6
3,268,142 - yecT NOLOHH_16620 0.17 -0.3
3,268,150 - yecT NOLOHH_16620 0.18 -0.5
3,268,480 + yecT NOLOHH_16620 0.86 -1.5
3,268,516 - -1.7
3,268,655 + yecV NOLOHH_16625 0.36 +0.1
3,268,661 + yecV NOLOHH_16625 0.49 +1.7
3,268,661 + yecV NOLOHH_16625 0.49 +0.9
3,268,661 + yecV NOLOHH_16625 0.49 +0.2
3,268,661 + yecV NOLOHH_16625 0.49 +0.2

Or see this region's nucleotide sequence