Strain Fitness in Escherichia coli ECOR27 around NOLOHH_16030

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpocR and NOLOHH_16030 are separated by 481 nucleotidesNOLOHH_16030 and cobU are separated by 133 nucleotidescobU and cobS overlap by 4 nucleotides NOLOHH_16025: pocR - regulatory protein PocR, at 3,150,315 to 3,151,229 pocR NOLOHH_16030: NOLOHH_16030 - cobalamin biosynthesis protein, at 3,151,711 to 3,152,148 _16030 NOLOHH_16035: cobU - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 3,152,282 to 3,152,827 cobU NOLOHH_16040: cobS - adenosylcobinamide-GDP ribazoletransferase, at 3,152,824 to 3,153,567 cobS Position (kb) 3151 3152 3153Strain fitness (log2 ratio) -2 -1 0 1at 3150.716 kb on + strand, within pocRat 3151.072 kb on + strand, within pocRat 3151.164 kb on + strandat 3151.227 kb on + strandat 3151.232 kb on + strandat 3151.276 kb on + strandat 3151.321 kb on + strandat 3151.326 kb on + strandat 3151.327 kb on - strandat 3151.594 kb on + strandat 3151.595 kb on - strandat 3151.649 kb on + strandat 3151.693 kb on - strandat 3151.693 kb on - strandat 3151.736 kb on + strandat 3151.794 kb on - strand, within NOLOHH_16030at 3151.891 kb on + strand, within NOLOHH_16030at 3151.904 kb on + strand, within NOLOHH_16030at 3151.970 kb on + strand, within NOLOHH_16030at 3152.252 kb on + strandat 3152.252 kb on + strandat 3152.260 kb on + strandat 3152.260 kb on + strandat 3152.260 kb on + strandat 3152.261 kb on - strandat 3152.261 kb on - strandat 3152.367 kb on - strand, within cobUat 3152.367 kb on - strand, within cobUat 3152.367 kb on - strand, within cobUat 3152.367 kb on - strand, within cobUat 3152.514 kb on + strand, within cobUat 3152.673 kb on + strand, within cobUat 3152.740 kb on + strand, within cobUat 3152.787 kb on + strandat 3152.787 kb on + strandat 3152.787 kb on + strandat 3152.787 kb on + strandat 3152.856 kb on + strandat 3152.911 kb on + strand, within cobSat 3152.925 kb on + strand, within cobSat 3152.939 kb on + strand, within cobSat 3153.095 kb on + strand, within cobSat 3153.108 kb on + strand, within cobS

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,150,716 + pocR NOLOHH_16025 0.44 +0.7
3,151,072 + pocR NOLOHH_16025 0.83 +0.8
3,151,164 + -0.0
3,151,227 + -2.5
3,151,232 + -0.4
3,151,276 + -0.1
3,151,321 + +0.1
3,151,326 + -0.8
3,151,327 - +0.7
3,151,594 + -0.4
3,151,595 - -1.4
3,151,649 + -0.6
3,151,693 - -0.1
3,151,693 - -1.9
3,151,736 + +0.3
3,151,794 - NOLOHH_16030 0.19 -2.5
3,151,891 + NOLOHH_16030 0.41 +0.0
3,151,904 + NOLOHH_16030 0.44 -0.6
3,151,970 + NOLOHH_16030 0.59 -0.2
3,152,252 + +0.0
3,152,252 + -0.5
3,152,260 + +1.1
3,152,260 + +0.1
3,152,260 + -0.9
3,152,261 - -0.0
3,152,261 - -0.1
3,152,367 - cobU NOLOHH_16035 0.16 +1.0
3,152,367 - cobU NOLOHH_16035 0.16 -0.4
3,152,367 - cobU NOLOHH_16035 0.16 -0.0
3,152,367 - cobU NOLOHH_16035 0.16 -0.1
3,152,514 + cobU NOLOHH_16035 0.42 -0.4
3,152,673 + cobU NOLOHH_16035 0.72 -0.4
3,152,740 + cobU NOLOHH_16035 0.84 +0.3
3,152,787 + +0.2
3,152,787 + -0.0
3,152,787 + -1.9
3,152,787 + +1.3
3,152,856 + -0.8
3,152,911 + cobS NOLOHH_16040 0.12 -1.9
3,152,925 + cobS NOLOHH_16040 0.14 +0.2
3,152,939 + cobS NOLOHH_16040 0.15 -0.1
3,153,095 + cobS NOLOHH_16040 0.36 -0.8
3,153,108 + cobS NOLOHH_16040 0.38 +0.0

Or see this region's nucleotide sequence