Strain Fitness in Escherichia coli ECOR27 around NOLOHH_15970

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpduM and NOLOHH_15965 overlap by 4 nucleotidesNOLOHH_15965 and NOLOHH_15970 are separated by 2 nucleotidesNOLOHH_15970 and pduJ are separated by 23 nucleotidespduJ and NOLOHH_15980 are separated by 19 nucleotidesNOLOHH_15980 and NOLOHH_15985 overlap by 11 nucleotides NOLOHH_15960: pduM - microcompartment protein PduM, at 3,140,758 to 3,141,249 pduM NOLOHH_15965: NOLOHH_15965 - propanediol utilization protein, at 3,141,246 to 3,141,878 _15965 NOLOHH_15970: NOLOHH_15970 - microcompartment protein, at 3,141,881 to 3,142,303 _15970 NOLOHH_15975: pduJ - propanediol utilization microcompartment protein PduJ, at 3,142,327 to 3,142,602 pduJ NOLOHH_15980: NOLOHH_15980 - propanediol dehydratase, at 3,142,622 to 3,142,972 _15980 NOLOHH_15985: NOLOHH_15985 - diol dehydratase reactivase subunit alpha, at 3,142,962 to 3,144,794 _15985 Position (kb) 3141 3142 3143Strain fitness (log2 ratio) -2 -1 0 1at 3140.903 kb on + strand, within pduMat 3140.903 kb on + strand, within pduMat 3140.936 kb on - strand, within pduMat 3141.109 kb on + strand, within pduMat 3141.120 kb on - strand, within pduMat 3141.321 kb on - strand, within NOLOHH_15965at 3141.434 kb on - strand, within NOLOHH_15965at 3141.434 kb on - strand, within NOLOHH_15965at 3141.458 kb on + strand, within NOLOHH_15965at 3141.458 kb on + strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.519 kb on + strand, within NOLOHH_15965at 3141.704 kb on - strand, within NOLOHH_15965at 3141.704 kb on - strand, within NOLOHH_15965at 3141.767 kb on - strand, within NOLOHH_15965at 3141.787 kb on - strand, within NOLOHH_15965at 3141.787 kb on - strand, within NOLOHH_15965at 3141.787 kb on - strand, within NOLOHH_15965at 3141.788 kb on + strand, within NOLOHH_15965at 3141.964 kb on + strand, within NOLOHH_15970at 3141.964 kb on + strand, within NOLOHH_15970at 3142.283 kb on + strandat 3142.316 kb on - strandat 3142.318 kb on + strandat 3142.319 kb on - strandat 3142.329 kb on - strandat 3142.329 kb on - strandat 3142.342 kb on + strandat 3142.348 kb on - strandat 3142.477 kb on - strand, within pduJat 3142.477 kb on - strand, within pduJat 3142.531 kb on - strand, within pduJat 3142.623 kb on + strandat 3142.656 kb on - strandat 3142.711 kb on - strand, within NOLOHH_15980at 3142.752 kb on + strand, within NOLOHH_15980at 3142.912 kb on + strand, within NOLOHH_15980at 3142.913 kb on - strand, within NOLOHH_15980at 3142.913 kb on - strand, within NOLOHH_15980at 3142.913 kb on - strand, within NOLOHH_15980at 3142.913 kb on - strand, within NOLOHH_15980at 3142.955 kb on - strandat 3142.955 kb on - strandat 3142.955 kb on - strandat 3142.964 kb on - strandat 3142.964 kb on - strandat 3143.010 kb on - strandat 3143.259 kb on + strand, within NOLOHH_15985at 3143.260 kb on - strand, within NOLOHH_15985

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,140,903 + pduM NOLOHH_15960 0.29 -1.9
3,140,903 + pduM NOLOHH_15960 0.29 -0.7
3,140,936 - pduM NOLOHH_15960 0.36 +0.2
3,141,109 + pduM NOLOHH_15960 0.71 -0.9
3,141,120 - pduM NOLOHH_15960 0.74 +0.1
3,141,321 - NOLOHH_15965 0.12 -0.5
3,141,434 - NOLOHH_15965 0.30 -0.1
3,141,434 - NOLOHH_15965 0.30 -0.4
3,141,458 + NOLOHH_15965 0.33 +0.4
3,141,458 + NOLOHH_15965 0.33 -2.1
3,141,459 - NOLOHH_15965 0.34 +0.4
3,141,459 - NOLOHH_15965 0.34 +0.2
3,141,459 - NOLOHH_15965 0.34 -0.3
3,141,459 - NOLOHH_15965 0.34 -1.3
3,141,459 - NOLOHH_15965 0.34 +0.6
3,141,459 - NOLOHH_15965 0.34 -0.5
3,141,459 - NOLOHH_15965 0.34 +0.3
3,141,459 - NOLOHH_15965 0.34 +0.1
3,141,519 + NOLOHH_15965 0.43 -1.1
3,141,704 - NOLOHH_15965 0.72 +1.1
3,141,704 - NOLOHH_15965 0.72 -0.2
3,141,767 - NOLOHH_15965 0.82 -0.5
3,141,787 - NOLOHH_15965 0.85 +0.3
3,141,787 - NOLOHH_15965 0.85 +0.6
3,141,787 - NOLOHH_15965 0.85 +0.3
3,141,788 + NOLOHH_15965 0.86 -0.6
3,141,964 + NOLOHH_15970 0.20 -1.3
3,141,964 + NOLOHH_15970 0.20 -0.0
3,142,283 + +0.3
3,142,316 - +0.6
3,142,318 + -0.6
3,142,319 - -0.4
3,142,329 - -0.1
3,142,329 - -1.5
3,142,342 + -0.6
3,142,348 - -0.2
3,142,477 - pduJ NOLOHH_15975 0.54 +0.1
3,142,477 - pduJ NOLOHH_15975 0.54 -2.1
3,142,531 - pduJ NOLOHH_15975 0.74 -0.4
3,142,623 + +0.5
3,142,656 - -0.3
3,142,711 - NOLOHH_15980 0.25 -0.1
3,142,752 + NOLOHH_15980 0.37 +0.5
3,142,912 + NOLOHH_15980 0.83 +0.4
3,142,913 - NOLOHH_15980 0.83 +0.1
3,142,913 - NOLOHH_15980 0.83 +1.0
3,142,913 - NOLOHH_15980 0.83 -0.2
3,142,913 - NOLOHH_15980 0.83 -0.9
3,142,955 - -0.9
3,142,955 - +0.3
3,142,955 - +0.6
3,142,964 - +0.7
3,142,964 - -0.6
3,143,010 - -0.0
3,143,259 + NOLOHH_15985 0.16 +1.2
3,143,260 - NOLOHH_15985 0.16 +0.5

Or see this region's nucleotide sequence