Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaJ and rfaB are separated by 8 nucleotides rfaB and rfaL are separated by 35 nucleotides
NOLOHH_07555: rfaJ - glucosyltransferase, at 1,436,528 to 1,437,541
rfaJ
NOLOHH_07560: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 1,437,550 to 1,438,692
rfaB
NOLOHH_07565: rfaL - O-antigen ligase RfaL, at 1,438,728 to 1,439,936
rfaL
Position (kb)
1437
1438
1439 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1436.749 kb on - strand, within rfaJ at 1437.136 kb on - strand, within rfaJ at 1437.321 kb on + strand, within rfaJ at 1437.328 kb on + strand, within rfaJ at 1437.523 kb on + strand at 1437.529 kb on + strand at 1437.717 kb on + strand, within rfaB at 1437.726 kb on + strand, within rfaB at 1437.870 kb on + strand, within rfaB at 1437.870 kb on + strand, within rfaB at 1437.883 kb on + strand, within rfaB at 1438.072 kb on + strand, within rfaB at 1438.114 kb on - strand, within rfaB at 1438.173 kb on - strand, within rfaB at 1438.230 kb on - strand, within rfaB at 1438.329 kb on + strand, within rfaB at 1438.366 kb on + strand, within rfaB at 1438.367 kb on - strand, within rfaB at 1438.470 kb on + strand, within rfaB at 1438.510 kb on - strand, within rfaB at 1438.533 kb on + strand, within rfaB at 1438.677 kb on + strand at 1438.846 kb on + strand at 1438.855 kb on + strand, within rfaL at 1438.906 kb on - strand, within rfaL at 1439.038 kb on + strand, within rfaL at 1439.048 kb on + strand, within rfaL at 1439.061 kb on - strand, within rfaL at 1439.098 kb on - strand, within rfaL at 1439.145 kb on - strand, within rfaL at 1439.236 kb on - strand, within rfaL at 1439.494 kb on + strand, within rfaL at 1439.495 kb on - strand, within rfaL at 1439.498 kb on - strand, within rfaL at 1439.608 kb on - strand, within rfaL at 1439.617 kb on - strand, within rfaL at 1439.624 kb on + strand, within rfaL at 1439.637 kb on - strand, within rfaL
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,436,749 - rfaJ NOLOHH_07555 0.22 -3.0 1,437,136 - rfaJ NOLOHH_07555 0.60 -4.7 1,437,321 + rfaJ NOLOHH_07555 0.78 -3.5 1,437,328 + rfaJ NOLOHH_07555 0.79 -4.2 1,437,523 + +1.1 1,437,529 + +0.8 1,437,717 + rfaB NOLOHH_07560 0.15 -3.7 1,437,726 + rfaB NOLOHH_07560 0.15 -2.7 1,437,870 + rfaB NOLOHH_07560 0.28 -3.5 1,437,870 + rfaB NOLOHH_07560 0.28 -5.0 1,437,883 + rfaB NOLOHH_07560 0.29 -4.2 1,438,072 + rfaB NOLOHH_07560 0.46 -5.1 1,438,114 - rfaB NOLOHH_07560 0.49 -2.0 1,438,173 - rfaB NOLOHH_07560 0.55 -4.6 1,438,230 - rfaB NOLOHH_07560 0.59 -2.0 1,438,329 + rfaB NOLOHH_07560 0.68 -3.5 1,438,366 + rfaB NOLOHH_07560 0.71 -3.1 1,438,367 - rfaB NOLOHH_07560 0.71 -4.5 1,438,470 + rfaB NOLOHH_07560 0.80 -4.2 1,438,510 - rfaB NOLOHH_07560 0.84 -3.6 1,438,533 + rfaB NOLOHH_07560 0.86 -3.3 1,438,677 + +0.1 1,438,846 + -5.3 1,438,855 + rfaL NOLOHH_07565 0.11 -5.0 1,438,906 - rfaL NOLOHH_07565 0.15 -4.4 1,439,038 + rfaL NOLOHH_07565 0.26 -3.5 1,439,048 + rfaL NOLOHH_07565 0.26 -4.1 1,439,061 - rfaL NOLOHH_07565 0.28 -5.6 1,439,098 - rfaL NOLOHH_07565 0.31 -4.4 1,439,145 - rfaL NOLOHH_07565 0.34 -3.5 1,439,236 - rfaL NOLOHH_07565 0.42 -3.9 1,439,494 + rfaL NOLOHH_07565 0.63 -2.9 1,439,495 - rfaL NOLOHH_07565 0.63 -4.6 1,439,498 - rfaL NOLOHH_07565 0.64 -3.2 1,439,608 - rfaL NOLOHH_07565 0.73 -2.7 1,439,617 - rfaL NOLOHH_07565 0.74 -5.3 1,439,624 + rfaL NOLOHH_07565 0.74 -4.0 1,439,637 - rfaL NOLOHH_07565 0.75 -3.0
Or see this region's nucleotide sequence