Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaG and rfaP overlap by 8 nucleotides rfaP and waaO are separated by 42 nucleotides waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides
NOLOHH_07535: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 1,432,806 to 1,433,930
rfaG
NOLOHH_07540: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 1,433,923 to 1,434,720
rfaP
NOLOHH_07545: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,434,763 to 1,435,770
waaO
NOLOHH_07550: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,435,796 to 1,436,503
rfaY
NOLOHH_07555: rfaJ - glucosyltransferase, at 1,436,528 to 1,437,541
rfaJ
Position (kb)
1434
1435
1436 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1433.856 kb on + strand at 1433.857 kb on - strand at 1433.864 kb on + strand at 1433.865 kb on - strand at 1433.865 kb on - strand at 1433.877 kb on + strand at 1433.877 kb on + strand at 1433.877 kb on + strand at 1433.934 kb on + strand at 1434.005 kb on + strand, within rfaP at 1434.193 kb on - strand, within rfaP at 1434.195 kb on + strand, within rfaP at 1434.195 kb on + strand, within rfaP at 1434.196 kb on - strand, within rfaP at 1434.199 kb on + strand, within rfaP at 1434.199 kb on + strand, within rfaP at 1434.200 kb on - strand, within rfaP at 1434.252 kb on + strand, within rfaP at 1434.252 kb on + strand, within rfaP at 1434.252 kb on + strand, within rfaP at 1434.281 kb on + strand, within rfaP at 1434.281 kb on + strand, within rfaP at 1434.281 kb on + strand, within rfaP at 1434.282 kb on - strand, within rfaP at 1434.296 kb on - strand, within rfaP at 1434.342 kb on + strand, within rfaP at 1434.348 kb on + strand, within rfaP at 1434.348 kb on + strand, within rfaP at 1434.348 kb on + strand, within rfaP at 1434.349 kb on - strand, within rfaP at 1434.351 kb on + strand, within rfaP at 1434.412 kb on + strand, within rfaP at 1434.412 kb on + strand, within rfaP at 1434.413 kb on - strand, within rfaP at 1434.413 kb on - strand, within rfaP at 1434.413 kb on - strand, within rfaP at 1434.627 kb on + strand, within rfaP at 1434.824 kb on + strand at 1434.848 kb on + strand at 1434.903 kb on + strand, within waaO at 1435.176 kb on + strand, within waaO at 1435.286 kb on + strand, within waaO at 1435.326 kb on + strand, within waaO at 1435.458 kb on + strand, within waaO at 1435.552 kb on + strand, within waaO at 1435.747 kb on + strand at 1435.802 kb on + strand at 1435.881 kb on + strand, within rfaY at 1435.972 kb on + strand, within rfaY at 1436.152 kb on + strand, within rfaY at 1436.166 kb on - strand, within rfaY at 1436.392 kb on + strand, within rfaY at 1436.430 kb on - strand, within rfaY at 1436.525 kb on - strand at 1436.525 kb on - strand at 1436.749 kb on - strand, within rfaJ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,433,856 + -4.3 1,433,857 - -1.3 1,433,864 + -1.0 1,433,865 - -3.3 1,433,865 - +0.4 1,433,877 + -0.3 1,433,877 + -0.7 1,433,877 + -0.2 1,433,934 + -4.2 1,434,005 + rfaP NOLOHH_07540 0.10 -2.2 1,434,193 - rfaP NOLOHH_07540 0.34 -1.7 1,434,195 + rfaP NOLOHH_07540 0.34 -3.6 1,434,195 + rfaP NOLOHH_07540 0.34 -1.0 1,434,196 - rfaP NOLOHH_07540 0.34 +0.4 1,434,199 + rfaP NOLOHH_07540 0.35 -2.8 1,434,199 + rfaP NOLOHH_07540 0.35 -3.0 1,434,200 - rfaP NOLOHH_07540 0.35 +0.1 1,434,252 + rfaP NOLOHH_07540 0.41 -0.8 1,434,252 + rfaP NOLOHH_07540 0.41 -2.0 1,434,252 + rfaP NOLOHH_07540 0.41 -1.6 1,434,281 + rfaP NOLOHH_07540 0.45 -2.0 1,434,281 + rfaP NOLOHH_07540 0.45 -1.2 1,434,281 + rfaP NOLOHH_07540 0.45 -3.2 1,434,282 - rfaP NOLOHH_07540 0.45 -2.7 1,434,296 - rfaP NOLOHH_07540 0.47 -2.8 1,434,342 + rfaP NOLOHH_07540 0.53 -1.6 1,434,348 + rfaP NOLOHH_07540 0.53 +0.4 1,434,348 + rfaP NOLOHH_07540 0.53 +0.0 1,434,348 + rfaP NOLOHH_07540 0.53 -1.9 1,434,349 - rfaP NOLOHH_07540 0.53 -2.8 1,434,351 + rfaP NOLOHH_07540 0.54 -1.0 1,434,412 + rfaP NOLOHH_07540 0.61 -2.2 1,434,412 + rfaP NOLOHH_07540 0.61 -3.3 1,434,413 - rfaP NOLOHH_07540 0.61 -1.5 1,434,413 - rfaP NOLOHH_07540 0.61 -1.3 1,434,413 - rfaP NOLOHH_07540 0.61 -0.7 1,434,627 + rfaP NOLOHH_07540 0.88 -0.4 1,434,824 + -2.2 1,434,848 + -3.5 1,434,903 + waaO NOLOHH_07545 0.14 -2.7 1,435,176 + waaO NOLOHH_07545 0.41 -0.4 1,435,286 + waaO NOLOHH_07545 0.52 -4.2 1,435,326 + waaO NOLOHH_07545 0.56 -3.4 1,435,458 + waaO NOLOHH_07545 0.69 -3.2 1,435,552 + waaO NOLOHH_07545 0.78 -3.9 1,435,747 + -3.6 1,435,802 + -2.2 1,435,881 + rfaY NOLOHH_07550 0.12 -0.1 1,435,972 + rfaY NOLOHH_07550 0.25 -0.3 1,436,152 + rfaY NOLOHH_07550 0.50 +0.1 1,436,166 - rfaY NOLOHH_07550 0.52 -4.0 1,436,392 + rfaY NOLOHH_07550 0.84 -0.0 1,436,430 - rfaY NOLOHH_07550 0.90 -3.7 1,436,525 - -2.9 1,436,525 - -3.7 1,436,749 - rfaJ NOLOHH_07555 0.22 -3.0
Or see this region's nucleotide sequence