Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD and yajC are separated by 60 nucleotides yajC and tgt are separated by 22 nucleotides tgt and queA are separated by 55 nucleotides
NOLOHH_01430: secD - Protein translocase subunit SecD, at 176,587 to 178,401
secD
NOLOHH_01435: yajC - preprotein translocase subunit YajC, at 178,462 to 178,794
yajC
NOLOHH_01440: tgt - tRNA guanosine(34) transglycosylase Tgt, at 178,817 to 179,944
tgt
NOLOHH_01445: queA - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 180,000 to 181,070
queA
Position (kb) 178
179
180 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 178.810 kb on - strand at 178.813 kb on - strand at 178.813 kb on - strand at 178.884 kb on - strand at 178.901 kb on - strand at 179.005 kb on - strand, within tgt at 179.005 kb on - strand, within tgt at 179.072 kb on - strand, within tgt at 179.072 kb on - strand, within tgt at 179.074 kb on - strand, within tgt at 179.074 kb on - strand, within tgt at 179.178 kb on - strand, within tgt at 179.203 kb on + strand, within tgt at 179.253 kb on - strand, within tgt at 179.253 kb on - strand, within tgt at 179.253 kb on - strand, within tgt at 179.253 kb on - strand, within tgt at 179.253 kb on - strand, within tgt at 179.276 kb on - strand, within tgt at 179.276 kb on - strand, within tgt at 179.276 kb on - strand, within tgt at 179.276 kb on - strand, within tgt at 179.276 kb on - strand, within tgt at 179.337 kb on - strand, within tgt at 179.337 kb on - strand, within tgt at 179.357 kb on - strand, within tgt at 179.509 kb on + strand, within tgt at 179.510 kb on - strand, within tgt at 179.639 kb on - strand, within tgt at 179.842 kb on - strand at 179.842 kb on - strand at 179.877 kb on - strand at 179.956 kb on + strand at 180.044 kb on + strand at 180.045 kb on - strand at 180.045 kb on - strand at 180.077 kb on - strand at 180.088 kb on - strand at 180.098 kb on + strand at 180.098 kb on + strand at 180.099 kb on - strand at 180.146 kb on + strand, within queA at 180.218 kb on - strand, within queA at 180.218 kb on - strand, within queA at 180.222 kb on - strand, within queA at 180.298 kb on - strand, within queA at 180.298 kb on - strand, within queA at 180.298 kb on - strand, within queA at 180.566 kb on - strand, within queA at 180.566 kb on - strand, within queA at 180.566 kb on - strand, within queA at 180.616 kb on + strand, within queA at 180.617 kb on - strand, within queA at 180.647 kb on + strand, within queA at 180.648 kb on - strand, within queA at 180.678 kb on - strand, within queA at 180.728 kb on + strand, within queA at 180.788 kb on + strand, within queA at 180.894 kb on - strand, within queA at 180.897 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 178,810 - -1.7 178,813 - -0.9 178,813 - -0.5 178,884 - -1.4 178,901 - +0.1 179,005 - tgt NOLOHH_01440 0.17 -2.5 179,005 - tgt NOLOHH_01440 0.17 -0.2 179,072 - tgt NOLOHH_01440 0.23 -2.8 179,072 - tgt NOLOHH_01440 0.23 +0.9 179,074 - tgt NOLOHH_01440 0.23 -2.1 179,074 - tgt NOLOHH_01440 0.23 +0.4 179,178 - tgt NOLOHH_01440 0.32 -1.7 179,203 + tgt NOLOHH_01440 0.34 -1.9 179,253 - tgt NOLOHH_01440 0.39 +1.0 179,253 - tgt NOLOHH_01440 0.39 -0.0 179,253 - tgt NOLOHH_01440 0.39 -1.9 179,253 - tgt NOLOHH_01440 0.39 -0.1 179,253 - tgt NOLOHH_01440 0.39 +0.5 179,276 - tgt NOLOHH_01440 0.41 -1.5 179,276 - tgt NOLOHH_01440 0.41 -0.1 179,276 - tgt NOLOHH_01440 0.41 -1.8 179,276 - tgt NOLOHH_01440 0.41 -2.8 179,276 - tgt NOLOHH_01440 0.41 -1.9 179,337 - tgt NOLOHH_01440 0.46 -1.4 179,337 - tgt NOLOHH_01440 0.46 -1.1 179,357 - tgt NOLOHH_01440 0.48 +1.4 179,509 + tgt NOLOHH_01440 0.61 -1.5 179,510 - tgt NOLOHH_01440 0.61 -1.6 179,639 - tgt NOLOHH_01440 0.73 +1.6 179,842 - -1.4 179,842 - -1.1 179,877 - -1.9 179,956 + +0.3 180,044 + -0.9 180,045 - -2.6 180,045 - +0.7 180,077 - -2.5 180,088 - -0.1 180,098 + +0.9 180,098 + -3.2 180,099 - +0.8 180,146 + queA NOLOHH_01445 0.14 -1.2 180,218 - queA NOLOHH_01445 0.20 -0.4 180,218 - queA NOLOHH_01445 0.20 +0.7 180,222 - queA NOLOHH_01445 0.21 -0.5 180,298 - queA NOLOHH_01445 0.28 -1.1 180,298 - queA NOLOHH_01445 0.28 +1.9 180,298 - queA NOLOHH_01445 0.28 -0.8 180,566 - queA NOLOHH_01445 0.53 -0.5 180,566 - queA NOLOHH_01445 0.53 -0.1 180,566 - queA NOLOHH_01445 0.53 -0.0 180,616 + queA NOLOHH_01445 0.58 -0.4 180,617 - queA NOLOHH_01445 0.58 -0.1 180,647 + queA NOLOHH_01445 0.60 +0.8 180,648 - queA NOLOHH_01445 0.61 -2.8 180,678 - queA NOLOHH_01445 0.63 -1.8 180,728 + queA NOLOHH_01445 0.68 +1.0 180,788 + queA NOLOHH_01445 0.74 -3.2 180,894 - queA NOLOHH_01445 0.83 -0.5 180,897 - queA NOLOHH_01445 0.84 -0.0
Or see this region's nucleotide sequence