Experiment: Experiment:PE, population C11, passage 1 (PE-S-C11-P1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pykF and LU632_RS11150 are separated by 23 nucleotides LU632_RS11150 and LU632_RS11155 are separated by 161 nucleotides LU632_RS11155 and LU632_RS11160 are separated by 9 nucleotides LU632_RS11160 and LU632_RS11165 are separated by 4 nucleotides LU632_RS11165 and LU632_RS11170 are separated by 148 nucleotides LU632_RS11170 and LU632_RS11175 are separated by 278 nucleotides
LU632_RS11145: pykF - pyruvate kinase PykF, at 2,133,674 to 2,135,086
pykF
LU632_RS11150: LU632_RS11150 - hypothetical protein, at 2,135,110 to 2,135,280
_RS11150
LU632_RS11155: LU632_RS11155 - tRNA-Val, at 2,135,442 to 2,135,518
_RS11155
LU632_RS11160: LU632_RS11160 - tRNA-Val, at 2,135,528 to 2,135,604
_RS11160
LU632_RS11165: LU632_RS11165 - tRNA-Val, at 2,135,609 to 2,135,685
_RS11165
LU632_RS11170: LU632_RS11170 - MATE family efflux transporter, at 2,135,834 to 2,137,207
_RS11170
LU632_RS11175: LU632_RS11175 - riboflavin synthase subunit alpha, at 2,137,486 to 2,138,139
_RS11175
Position (kb)
2135
2136
2137
2138 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2134.942 kb on + strand, within pykF at 2135.006 kb on - strand at 2135.095 kb on - strand at 2135.101 kb on + strand at 2135.240 kb on - strand, within LU632_RS11150 at 2135.324 kb on + strand at 2135.374 kb on + strand at 2135.452 kb on + strand, within LU632_RS11155 at 2135.510 kb on + strand, within LU632_RS11155 at 2135.882 kb on - strand at 2135.890 kb on + strand at 2135.933 kb on - strand at 2135.997 kb on + strand, within LU632_RS11170 at 2136.025 kb on + strand, within LU632_RS11170 at 2136.037 kb on + strand, within LU632_RS11170 at 2136.050 kb on - strand, within LU632_RS11170 at 2136.178 kb on - strand, within LU632_RS11170 at 2136.381 kb on - strand, within LU632_RS11170 at 2136.506 kb on + strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.526 kb on + strand, within LU632_RS11170 at 2136.526 kb on + strand, within LU632_RS11170 at 2136.787 kb on - strand, within LU632_RS11170 at 2136.819 kb on + strand, within LU632_RS11170 at 2136.888 kb on + strand, within LU632_RS11170 at 2136.888 kb on + strand, within LU632_RS11170 at 2136.921 kb on - strand, within LU632_RS11170 at 2136.933 kb on + strand, within LU632_RS11170 at 2136.984 kb on + strand, within LU632_RS11170 at 2136.984 kb on + strand, within LU632_RS11170 at 2136.984 kb on + strand, within LU632_RS11170 at 2136.985 kb on - strand, within LU632_RS11170 at 2136.985 kb on - strand, within LU632_RS11170 at 2136.985 kb on - strand, within LU632_RS11170 at 2136.996 kb on - strand, within LU632_RS11170 at 2136.996 kb on - strand, within LU632_RS11170 at 2137.036 kb on + strand, within LU632_RS11170 at 2137.036 kb on + strand, within LU632_RS11170 at 2137.160 kb on + strand at 2137.162 kb on + strand at 2137.211 kb on + strand at 2137.212 kb on - strand at 2137.212 kb on - strand at 2137.212 kb on - strand at 2137.272 kb on - strand at 2137.272 kb on - strand at 2137.272 kb on - strand at 2137.343 kb on + strand at 2137.368 kb on - strand at 2137.368 kb on - strand at 2137.368 kb on - strand at 2137.372 kb on + strand at 2137.386 kb on + strand at 2137.387 kb on - strand at 2137.431 kb on + strand at 2138.137 kb on + strand at 2138.138 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:PE, population C11, passage 1 (PE-S-C11-P1) remove 2,134,942 + pykF LU632_RS11145 0.90 -0.4 2,135,006 - -0.3 2,135,095 - -2.5 2,135,101 + +0.8 2,135,240 - LU632_RS11150 0.76 +2.5 2,135,324 + +0.0 2,135,374 + +1.3 2,135,452 + LU632_RS11155 0.13 -3.0 2,135,510 + LU632_RS11155 0.88 +0.4 2,135,882 - +1.1 2,135,890 + +0.6 2,135,933 - +3.6 2,135,997 + LU632_RS11170 0.12 +0.4 2,136,025 + LU632_RS11170 0.14 +0.1 2,136,037 + LU632_RS11170 0.15 -0.6 2,136,050 - LU632_RS11170 0.16 +1.9 2,136,178 - LU632_RS11170 0.25 +1.2 2,136,381 - LU632_RS11170 0.40 +3.5 2,136,506 + LU632_RS11170 0.49 -0.6 2,136,507 - LU632_RS11170 0.49 +2.2 2,136,507 - LU632_RS11170 0.49 +1.3 2,136,507 - LU632_RS11170 0.49 -0.8 2,136,507 - LU632_RS11170 0.49 -0.0 2,136,526 + LU632_RS11170 0.50 +1.5 2,136,526 + LU632_RS11170 0.50 -0.6 2,136,787 - LU632_RS11170 0.69 +1.5 2,136,819 + LU632_RS11170 0.72 +2.3 2,136,888 + LU632_RS11170 0.77 +0.1 2,136,888 + LU632_RS11170 0.77 -1.9 2,136,921 - LU632_RS11170 0.79 -3.2 2,136,933 + LU632_RS11170 0.80 +1.2 2,136,984 + LU632_RS11170 0.84 +1.0 2,136,984 + LU632_RS11170 0.84 +1.2 2,136,984 + LU632_RS11170 0.84 -2.3 2,136,985 - LU632_RS11170 0.84 +0.1 2,136,985 - LU632_RS11170 0.84 +1.9 2,136,985 - LU632_RS11170 0.84 -1.5 2,136,996 - LU632_RS11170 0.85 +1.4 2,136,996 - LU632_RS11170 0.85 +1.7 2,137,036 + LU632_RS11170 0.87 +1.4 2,137,036 + LU632_RS11170 0.87 -0.6 2,137,160 + +1.9 2,137,162 + +0.2 2,137,211 + -0.7 2,137,212 - +1.0 2,137,212 - +2.6 2,137,212 - -1.3 2,137,272 - -0.1 2,137,272 - -0.4 2,137,272 - +0.3 2,137,343 + +0.7 2,137,368 - +1.3 2,137,368 - +1.4 2,137,368 - -2.6 2,137,372 + -1.5 2,137,386 + -1.6 2,137,387 - -2.6 2,137,431 + -0.4 2,138,137 + -0.1 2,138,138 - -3.8
Or see this region's nucleotide sequence