Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lolE and nagK are separated by 28 nucleotides nagK and cobB are separated by 15 nucleotides cobB and potD are separated by 155 nucleotides
NIAGMN_24380: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 4,770,199 to 4,771,437
lolE
NIAGMN_24385: nagK - N-acetylglucosamine kinase, at 4,771,466 to 4,772,377
nagK
NIAGMN_24390: cobB - NAD-dependent protein deacylase, at 4,772,393 to 4,773,214
cobB
NIAGMN_24395: potD - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 4,773,370 to 4,774,416
potD
Position (kb)
4772
4773
4774 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4771.444 kb on + strand at 4771.444 kb on + strand at 4771.445 kb on - strand at 4771.449 kb on + strand at 4771.449 kb on + strand at 4771.450 kb on - strand at 4771.469 kb on + strand at 4771.571 kb on + strand, within nagK at 4771.633 kb on + strand, within nagK at 4771.764 kb on + strand, within nagK at 4772.067 kb on - strand, within nagK at 4772.070 kb on - strand, within nagK at 4772.108 kb on + strand, within nagK at 4772.292 kb on + strand at 4772.361 kb on + strand at 4772.785 kb on - strand, within cobB at 4772.908 kb on + strand, within cobB at 4772.953 kb on - strand, within cobB at 4772.993 kb on + strand, within cobB at 4773.210 kb on + strand at 4773.212 kb on + strand at 4773.372 kb on - strand at 4773.398 kb on + strand at 4773.408 kb on - strand at 4774.032 kb on - strand, within potD at 4774.032 kb on - strand, within potD at 4774.161 kb on - strand, within potD
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 4,771,444 + -0.4 4,771,444 + -2.1 4,771,445 - +0.2 4,771,449 + +3.6 4,771,449 + +0.4 4,771,450 - +0.4 4,771,469 + -0.4 4,771,571 + nagK NIAGMN_24385 0.12 -3.6 4,771,633 + nagK NIAGMN_24385 0.18 -1.4 4,771,764 + nagK NIAGMN_24385 0.33 +0.7 4,772,067 - nagK NIAGMN_24385 0.66 -0.6 4,772,070 - nagK NIAGMN_24385 0.66 +0.6 4,772,108 + nagK NIAGMN_24385 0.70 -1.5 4,772,292 + -0.0 4,772,361 + -1.4 4,772,785 - cobB NIAGMN_24390 0.48 +1.3 4,772,908 + cobB NIAGMN_24390 0.63 -1.3 4,772,953 - cobB NIAGMN_24390 0.68 -1.8 4,772,993 + cobB NIAGMN_24390 0.73 -0.4 4,773,210 + +1.2 4,773,212 + +0.3 4,773,372 - -2.6 4,773,398 + -0.0 4,773,408 - +0.9 4,774,032 - potD NIAGMN_24395 0.63 +2.6 4,774,032 - potD NIAGMN_24395 0.63 +2.3 4,774,161 - potD NIAGMN_24395 0.76 +1.7
Or see this region's nucleotide sequence