Experiment: T2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tar and cheW are separated by 142 nucleotides cheW and cheA are separated by 20 nucleotides cheA and motB are separated by 4 nucleotides motB and motA overlap by 4 nucleotides
NIAGMN_01925: tar - methyl-accepting chemotaxis protein II, at 348,542 to 350,203
tar
NIAGMN_01935: cheW - chemotaxis protein CheW, at 350,346 to 350,849
cheW
NIAGMN_01940: cheA - chemotaxis protein CheA, at 350,870 to 352,834
cheA
NIAGMN_01945: motB - flagellar motor protein MotB, at 352,839 to 353,765
motB
NIAGMN_01950: motA - flagellar motor stator protein MotA, at 353,762 to 354,649
motA
Position (kb) 350
351
352
353 Strain fitness (log2 ratio) -1
0
1 at 350.203 kb on + strand at 350.430 kb on - strand, within cheW at 350.589 kb on + strand, within cheW at 350.589 kb on + strand, within cheW at 350.606 kb on - strand, within cheW at 350.850 kb on - strand at 350.891 kb on - strand at 350.995 kb on - strand at 350.999 kb on - strand at 351.039 kb on - strand at 351.039 kb on - strand at 351.039 kb on - strand at 351.395 kb on - strand, within cheA at 351.734 kb on + strand, within cheA at 351.844 kb on - strand, within cheA at 351.905 kb on + strand, within cheA at 352.010 kb on - strand, within cheA at 352.452 kb on - strand, within cheA at 352.482 kb on + strand, within cheA at 352.482 kb on + strand, within cheA at 352.821 kb on - strand at 352.821 kb on - strand at 353.196 kb on - strand, within motB
Per-strain Table
Position Strand Gene LocusTag Fraction T2 remove 350,203 + -0.4 350,430 - cheW NIAGMN_01935 0.17 +0.4 350,589 + cheW NIAGMN_01935 0.48 -0.4 350,589 + cheW NIAGMN_01935 0.48 -1.0 350,606 - cheW NIAGMN_01935 0.52 -0.9 350,850 - +0.3 350,891 - -1.0 350,995 - -0.7 350,999 - -0.2 351,039 - +0.3 351,039 - -0.7 351,039 - -1.0 351,395 - cheA NIAGMN_01940 0.27 -0.6 351,734 + cheA NIAGMN_01940 0.44 +0.3 351,844 - cheA NIAGMN_01940 0.50 +0.9 351,905 + cheA NIAGMN_01940 0.53 -0.5 352,010 - cheA NIAGMN_01940 0.58 +0.0 352,452 - cheA NIAGMN_01940 0.81 -0.7 352,482 + cheA NIAGMN_01940 0.82 +0.1 352,482 + cheA NIAGMN_01940 0.82 +0.5 352,821 - +0.3 352,821 - +0.1 353,196 - motB NIAGMN_01945 0.39 -0.2
Or see this region's nucleotide sequence