Strain Fitness in Escherichia coli ECOR27 around NOLOHH_24465

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthilD and NOLOHH_24455 are separated by 13 nucleotidesNOLOHH_24455 and NOLOHH_24460 are separated by 55 nucleotidesNOLOHH_24460 and NOLOHH_24465 are separated by 20 nucleotidesNOLOHH_24465 and NOLOHH_24470 are separated by 60 nucleotides NOLOHH_24450: hilD - transcriptional regulator HilD, at 35,769 to 36,665 hilD NOLOHH_24455: NOLOHH_24455 - lytic transglycosylase, at 36,679 to 37,122 _24455 NOLOHH_24460: NOLOHH_24460 - LngX2, at 37,178 to 37,399 _24460 NOLOHH_24465: NOLOHH_24465 - Longus pilus structural subunit, at 37,420 to 38,133 _24465 NOLOHH_24470: NOLOHH_24470 - prepilin-type cleavage/methylation domain-containing protein, at 38,194 to 39,765 _24470 Position (kb) 37 38 39Strain fitness (log2 ratio) -1 0 1at 36.611 kb on + strandat 37.296 kb on + strand, within NOLOHH_24460at 37.298 kb on + strand, within NOLOHH_24460at 37.569 kb on - strand, within NOLOHH_24465at 37.637 kb on + strand, within NOLOHH_24465at 37.637 kb on + strand, within NOLOHH_24465at 37.638 kb on - strand, within NOLOHH_24465at 37.660 kb on + strand, within NOLOHH_24465at 37.881 kb on - strand, within NOLOHH_24465at 38.066 kb on + strandat 38.176 kb on + strandat 38.271 kb on + strandat 38.276 kb on - strandat 38.568 kb on + strand, within NOLOHH_24470

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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36,611 + +0.9
37,296 + NOLOHH_24460 0.53 +0.3
37,298 + NOLOHH_24460 0.54 +0.9
37,569 - NOLOHH_24465 0.21 +0.9
37,637 + NOLOHH_24465 0.30 -1.6
37,637 + NOLOHH_24465 0.30 -0.6
37,638 - NOLOHH_24465 0.31 -0.6
37,660 + NOLOHH_24465 0.34 -1.2
37,881 - NOLOHH_24465 0.65 +0.7
38,066 + -0.9
38,176 + -1.6
38,271 + +1.0
38,276 - -1.1
38,568 + NOLOHH_24470 0.24 -0.6

Or see this region's nucleotide sequence