Strain Fitness in Escherichia coli ECOR27 around NOLOHH_22440

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdgcI and pdeI are separated by 7 nucleotidespdeI and gsiD are separated by 177 nucleotides NOLOHH_22435: dgcI - putative diguanylate cyclase DgcI, at 4,426,401 to 4,427,729 dgcI NOLOHH_22440: pdeI - putative cyclic di-GMP phosphodiesterase PdeI, at 4,427,737 to 4,430,085 pdeI NOLOHH_22445: gsiD - glutathione ABC transporter permease GsiD, at 4,430,263 to 4,431,174 gsiD Position (kb) 4427 4428 4429 4430 4431Strain fitness (log2 ratio) -2 -1 0 1at 4426.854 kb on - strand, within dgcIat 4426.856 kb on - strand, within dgcIat 4427.378 kb on + strand, within dgcIat 4427.577 kb on - strand, within dgcIat 4427.702 kb on + strandat 4427.730 kb on - strandat 4427.825 kb on - strandat 4428.427 kb on - strand, within pdeIat 4428.430 kb on - strand, within pdeIat 4428.487 kb on - strand, within pdeIat 4430.017 kb on - strandat 4430.017 kb on - strandat 4430.136 kb on + strandat 4430.261 kb on - strandat 4430.264 kb on + strandat 4430.340 kb on + strandat 4430.536 kb on - strand, within gsiDat 4430.572 kb on - strand, within gsiDat 4431.040 kb on + strand, within gsiD

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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4,426,854 - dgcI NOLOHH_22435 0.34 +1.4
4,426,856 - dgcI NOLOHH_22435 0.34 -1.2
4,427,378 + dgcI NOLOHH_22435 0.74 +0.1
4,427,577 - dgcI NOLOHH_22435 0.88 +0.5
4,427,702 + -0.8
4,427,730 - -0.2
4,427,825 - +0.5
4,428,427 - pdeI NOLOHH_22440 0.29 -0.4
4,428,430 - pdeI NOLOHH_22440 0.30 -1.1
4,428,487 - pdeI NOLOHH_22440 0.32 -1.7
4,430,017 - +0.1
4,430,017 - +1.4
4,430,136 + +0.5
4,430,261 - -1.2
4,430,264 + -0.2
4,430,340 + -0.3
4,430,536 - gsiD NOLOHH_22445 0.30 -1.9
4,430,572 - gsiD NOLOHH_22445 0.34 -1.3
4,431,040 + gsiD NOLOHH_22445 0.85 -0.5

Or see this region's nucleotide sequence