Strain Fitness in Escherichia coli ECOR27 around NOLOHH_21035

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntndh and ycfP are separated by 407 nucleotidesycfP and nagZ are separated by 22 nucleotidesnagZ and thiK are separated by 10 nucleotidesthiK and lpoB overlap by 20 nucleotides NOLOHH_21020: ndh - NADH-quinone dehydrogenase, at 4,136,689 to 4,137,993 ndh NOLOHH_21030: ycfP - alpha/beta hydrolase YcfP, at 4,138,401 to 4,138,943 ycfP NOLOHH_21035: nagZ - beta-N-acetylhexosaminidase, at 4,138,966 to 4,139,991 nagZ NOLOHH_21040: thiK - thiamine kinase, at 4,140,002 to 4,140,826 thiK NOLOHH_21045: lpoB - penicillin-binding protein activator LpoB, at 4,140,807 to 4,141,448 lpoB Position (kb) 4138 4139 4140Strain fitness (log2 ratio) -2 -1 0 1 2at 4138.231 kb on + strandat 4138.248 kb on + strandat 4138.435 kb on - strandat 4138.449 kb on - strandat 4138.605 kb on - strand, within ycfPat 4138.839 kb on - strand, within ycfPat 4138.844 kb on - strand, within ycfPat 4138.849 kb on - strand, within ycfPat 4138.934 kb on - strandat 4138.956 kb on + strandat 4139.122 kb on - strand, within nagZat 4139.146 kb on - strand, within nagZat 4139.152 kb on - strand, within nagZat 4139.210 kb on + strand, within nagZat 4139.346 kb on - strand, within nagZat 4139.412 kb on + strand, within nagZat 4139.413 kb on - strand, within nagZat 4139.413 kb on - strand, within nagZat 4139.413 kb on - strand, within nagZat 4139.413 kb on - strand, within nagZat 4139.867 kb on - strand, within nagZat 4139.933 kb on - strandat 4139.933 kb on - strandat 4139.933 kb on - strandat 4140.017 kb on + strandat 4140.017 kb on + strandat 4140.018 kb on - strandat 4140.178 kb on - strand, within thiKat 4140.262 kb on - strand, within thiKat 4140.326 kb on - strand, within thiKat 4140.339 kb on - strand, within thiKat 4140.489 kb on - strand, within thiKat 4140.492 kb on + strand, within thiKat 4140.493 kb on - strand, within thiKat 4140.493 kb on - strand, within thiKat 4140.495 kb on + strand, within thiKat 4140.496 kb on - strand, within thiKat 4140.582 kb on + strand, within thiKat 4140.582 kb on + strand, within thiKat 4140.584 kb on + strand, within thiKat 4140.584 kb on + strand, within thiKat 4140.585 kb on - strand, within thiKat 4140.585 kb on - strand, within thiKat 4140.585 kb on - strand, within thiKat 4140.615 kb on - strand, within thiKat 4140.842 kb on + strandat 4140.880 kb on - strand, within lpoBat 4140.917 kb on - strand, within lpoBat 4140.926 kb on - strand, within lpoB

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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4,138,231 + +0.1
4,138,248 + -0.5
4,138,435 - -0.0
4,138,449 - +0.2
4,138,605 - ycfP NOLOHH_21030 0.38 +2.0
4,138,839 - ycfP NOLOHH_21030 0.81 +0.4
4,138,844 - ycfP NOLOHH_21030 0.82 -0.2
4,138,849 - ycfP NOLOHH_21030 0.83 -0.7
4,138,934 - +0.8
4,138,956 + -1.3
4,139,122 - nagZ NOLOHH_21035 0.15 +0.6
4,139,146 - nagZ NOLOHH_21035 0.18 +0.1
4,139,152 - nagZ NOLOHH_21035 0.18 -0.9
4,139,210 + nagZ NOLOHH_21035 0.24 +0.1
4,139,346 - nagZ NOLOHH_21035 0.37 -1.2
4,139,412 + nagZ NOLOHH_21035 0.43 -1.7
4,139,413 - nagZ NOLOHH_21035 0.44 -0.2
4,139,413 - nagZ NOLOHH_21035 0.44 +2.0
4,139,413 - nagZ NOLOHH_21035 0.44 -1.3
4,139,413 - nagZ NOLOHH_21035 0.44 -1.7
4,139,867 - nagZ NOLOHH_21035 0.88 -0.6
4,139,933 - +0.1
4,139,933 - -0.4
4,139,933 - +0.9
4,140,017 + -1.0
4,140,017 + -0.7
4,140,018 - +0.4
4,140,178 - thiK NOLOHH_21040 0.21 -0.0
4,140,262 - thiK NOLOHH_21040 0.32 -0.1
4,140,326 - thiK NOLOHH_21040 0.39 -0.2
4,140,339 - thiK NOLOHH_21040 0.41 +0.7
4,140,489 - thiK NOLOHH_21040 0.59 -1.9
4,140,492 + thiK NOLOHH_21040 0.59 -0.6
4,140,493 - thiK NOLOHH_21040 0.60 -0.9
4,140,493 - thiK NOLOHH_21040 0.60 -0.3
4,140,495 + thiK NOLOHH_21040 0.60 +0.3
4,140,496 - thiK NOLOHH_21040 0.60 -0.5
4,140,582 + thiK NOLOHH_21040 0.70 -0.3
4,140,582 + thiK NOLOHH_21040 0.70 -1.5
4,140,584 + thiK NOLOHH_21040 0.71 -1.3
4,140,584 + thiK NOLOHH_21040 0.71 -0.7
4,140,585 - thiK NOLOHH_21040 0.71 +0.3
4,140,585 - thiK NOLOHH_21040 0.71 +2.5
4,140,585 - thiK NOLOHH_21040 0.71 +0.9
4,140,615 - thiK NOLOHH_21040 0.74 -0.3
4,140,842 + -1.2
4,140,880 - lpoB NOLOHH_21045 0.11 +1.1
4,140,917 - lpoB NOLOHH_21045 0.17 +0.7
4,140,926 - lpoB NOLOHH_21045 0.19 +0.4

Or see this region's nucleotide sequence