Experiment: RB51
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acnA and ymiC are separated by 163 nucleotides ymiC and yciX are separated by 113 nucleotides yciX and ymiA are separated by 2 nucleotides ymiA and cysB are separated by 330 nucleotides
NOLOHH_20240: acnA - aconitate hydratase AcnA, at 3,985,334 to 3,988,009
acnA
NOLOHH_20250: ymiC - Protein YmiC, at 3,988,173 to 3,988,268
ymiC
NOLOHH_20255: yciX - YmiA family putative membrane protein, at 3,988,382 to 3,988,549
yciX
NOLOHH_20260: ymiA - YmiA family putative membrane protein, at 3,988,552 to 3,988,680
ymiA
NOLOHH_20265: cysB - HTH-type transcriptional regulator CysB, at 3,989,011 to 3,989,985
cysB
Position (kb)
3988
3989 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3987.263 kb on + strand, within acnA at 3987.263 kb on + strand, within acnA at 3987.264 kb on - strand, within acnA at 3987.450 kb on + strand, within acnA at 3987.845 kb on - strand at 3987.922 kb on + strand at 3987.945 kb on + strand at 3988.062 kb on - strand at 3988.247 kb on - strand, within ymiC at 3988.247 kb on - strand, within ymiC at 3988.292 kb on - strand at 3988.292 kb on - strand at 3988.333 kb on - strand at 3988.421 kb on - strand, within yciX at 3988.515 kb on - strand, within yciX at 3988.550 kb on - strand at 3988.550 kb on - strand at 3988.550 kb on - strand at 3988.685 kb on - strand at 3988.943 kb on + strand at 3989.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB51 remove 3,987,263 + acnA NOLOHH_20240 0.72 +0.3 3,987,263 + acnA NOLOHH_20240 0.72 +0.1 3,987,264 - acnA NOLOHH_20240 0.72 -1.0 3,987,450 + acnA NOLOHH_20240 0.79 +0.9 3,987,845 - -0.3 3,987,922 + -1.0 3,987,945 + -0.5 3,988,062 - +0.2 3,988,247 - ymiC NOLOHH_20250 0.77 +0.1 3,988,247 - ymiC NOLOHH_20250 0.77 -1.5 3,988,292 - -0.8 3,988,292 - +0.1 3,988,333 - -1.2 3,988,421 - yciX NOLOHH_20255 0.23 +0.7 3,988,515 - yciX NOLOHH_20255 0.79 +3.4 3,988,550 - +0.4 3,988,550 - +0.4 3,988,550 - +1.1 3,988,685 - -1.0 3,988,943 + -0.7 3,989,030 - +0.7
Or see this region's nucleotide sequence