Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20250

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacnA and ymiC are separated by 163 nucleotidesymiC and yciX are separated by 113 nucleotidesyciX and ymiA are separated by 2 nucleotidesymiA and cysB are separated by 330 nucleotides NOLOHH_20240: acnA - aconitate hydratase AcnA, at 3,985,334 to 3,988,009 acnA NOLOHH_20250: ymiC - Protein YmiC, at 3,988,173 to 3,988,268 ymiC NOLOHH_20255: yciX - YmiA family putative membrane protein, at 3,988,382 to 3,988,549 yciX NOLOHH_20260: ymiA - YmiA family putative membrane protein, at 3,988,552 to 3,988,680 ymiA NOLOHH_20265: cysB - HTH-type transcriptional regulator CysB, at 3,989,011 to 3,989,985 cysB Position (kb) 3988 3989Strain fitness (log2 ratio) -1 0 1 2 3at 3987.263 kb on + strand, within acnAat 3987.263 kb on + strand, within acnAat 3987.264 kb on - strand, within acnAat 3987.450 kb on + strand, within acnAat 3987.845 kb on - strandat 3987.922 kb on + strandat 3987.945 kb on + strandat 3988.062 kb on - strandat 3988.247 kb on - strand, within ymiCat 3988.247 kb on - strand, within ymiCat 3988.292 kb on - strandat 3988.292 kb on - strandat 3988.333 kb on - strandat 3988.421 kb on - strand, within yciXat 3988.515 kb on - strand, within yciXat 3988.550 kb on - strandat 3988.550 kb on - strandat 3988.550 kb on - strandat 3988.685 kb on - strandat 3988.943 kb on + strandat 3989.030 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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3,987,263 + acnA NOLOHH_20240 0.72 +0.3
3,987,263 + acnA NOLOHH_20240 0.72 +0.1
3,987,264 - acnA NOLOHH_20240 0.72 -1.0
3,987,450 + acnA NOLOHH_20240 0.79 +0.9
3,987,845 - -0.3
3,987,922 + -1.0
3,987,945 + -0.5
3,988,062 - +0.2
3,988,247 - ymiC NOLOHH_20250 0.77 +0.1
3,988,247 - ymiC NOLOHH_20250 0.77 -1.5
3,988,292 - -0.8
3,988,292 - +0.1
3,988,333 - -1.2
3,988,421 - yciX NOLOHH_20255 0.23 +0.7
3,988,515 - yciX NOLOHH_20255 0.79 +3.4
3,988,550 - +0.4
3,988,550 - +0.4
3,988,550 - +1.1
3,988,685 - -1.0
3,988,943 + -0.7
3,989,030 - +0.7

Or see this region's nucleotide sequence