Experiment: RB51
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt puuC and puuR are separated by 274 nucleotides puuR and puuD are separated by 26 nucleotides
NOLOHH_20115: puuC - aldehyde dehydrogenase PuuC, at 3,959,609 to 3,961,096
puuC
NOLOHH_20120: puuR - HTH-type transcriptional regulator PuuR, at 3,961,371 to 3,961,928
puuR
NOLOHH_20125: puuD - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 3,961,955 to 3,962,719
puuD
Position (kb)
3961
3962 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3960.819 kb on - strand, within puuC at 3961.013 kb on - strand at 3961.013 kb on - strand at 3961.125 kb on - strand at 3961.127 kb on - strand at 3961.127 kb on - strand at 3961.127 kb on - strand at 3961.127 kb on - strand at 3961.127 kb on - strand at 3961.202 kb on - strand at 3961.322 kb on + strand at 3961.323 kb on - strand at 3961.325 kb on - strand at 3961.356 kb on + strand at 3961.557 kb on - strand, within puuR at 3961.753 kb on + strand, within puuR at 3961.753 kb on + strand, within puuR at 3961.754 kb on - strand, within puuR at 3961.779 kb on - strand, within puuR at 3961.779 kb on - strand, within puuR at 3961.800 kb on - strand, within puuR at 3961.810 kb on - strand, within puuR at 3961.821 kb on - strand, within puuR at 3961.929 kb on - strand at 3961.950 kb on - strand at 3961.979 kb on - strand at 3962.016 kb on - strand at 3962.016 kb on - strand at 3962.222 kb on + strand, within puuD at 3962.262 kb on + strand, within puuD at 3962.263 kb on - strand, within puuD at 3962.424 kb on - strand, within puuD at 3962.486 kb on + strand, within puuD at 3962.486 kb on + strand, within puuD at 3962.486 kb on + strand, within puuD at 3962.486 kb on + strand, within puuD at 3962.487 kb on - strand, within puuD at 3962.487 kb on - strand, within puuD at 3962.487 kb on - strand, within puuD at 3962.487 kb on - strand, within puuD at 3962.524 kb on - strand, within puuD at 3962.663 kb on - strand at 3962.777 kb on - strand at 3962.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB51 remove 3,960,819 - puuC NOLOHH_20115 0.81 +0.5 3,961,013 - -0.3 3,961,013 - +0.8 3,961,125 - +1.0 3,961,127 - -1.7 3,961,127 - +1.4 3,961,127 - -0.8 3,961,127 - -0.8 3,961,127 - +0.9 3,961,202 - +0.3 3,961,322 + -1.0 3,961,323 - +0.0 3,961,325 - +0.7 3,961,356 + +1.0 3,961,557 - puuR NOLOHH_20120 0.33 -0.8 3,961,753 + puuR NOLOHH_20120 0.68 -0.9 3,961,753 + puuR NOLOHH_20120 0.68 +0.7 3,961,754 - puuR NOLOHH_20120 0.69 -0.3 3,961,779 - puuR NOLOHH_20120 0.73 -0.8 3,961,779 - puuR NOLOHH_20120 0.73 -0.6 3,961,800 - puuR NOLOHH_20120 0.77 +0.4 3,961,810 - puuR NOLOHH_20120 0.79 +0.3 3,961,821 - puuR NOLOHH_20120 0.81 -1.8 3,961,929 - -0.7 3,961,950 - +1.1 3,961,979 - -0.8 3,962,016 - -0.8 3,962,016 - +1.9 3,962,222 + puuD NOLOHH_20125 0.35 +0.3 3,962,262 + puuD NOLOHH_20125 0.40 +2.0 3,962,263 - puuD NOLOHH_20125 0.40 +1.6 3,962,424 - puuD NOLOHH_20125 0.61 +0.8 3,962,486 + puuD NOLOHH_20125 0.69 +2.0 3,962,486 + puuD NOLOHH_20125 0.69 -0.2 3,962,486 + puuD NOLOHH_20125 0.69 +0.5 3,962,486 + puuD NOLOHH_20125 0.69 +0.1 3,962,487 - puuD NOLOHH_20125 0.70 -1.5 3,962,487 - puuD NOLOHH_20125 0.70 -1.5 3,962,487 - puuD NOLOHH_20125 0.70 -0.3 3,962,487 - puuD NOLOHH_20125 0.70 +0.0 3,962,524 - puuD NOLOHH_20125 0.74 +1.3 3,962,663 - -0.1 3,962,777 - +1.0 3,962,880 + +0.2
Or see this region's nucleotide sequence