Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt traV and trbG overlap by 4 nucleotides trbG and NOLOHH_24820 are separated by 11 nucleotides NOLOHH_24820 and NOLOHH_24825 overlap by 14 nucleotides NOLOHH_24825 and traB overlap by 11 nucleotides
NOLOHH_24810: traV - type IV conjugative transfer system lipoprotein TraV, at 100,590 to 101,105
traV
NOLOHH_24815: trbG - conjugal transfer protein TrbG, at 101,102 to 101,353
trbG
NOLOHH_24820: NOLOHH_24820 - Conjugal transfer protein TrbD, at 101,365 to 101,562
_24820
NOLOHH_24825: NOLOHH_24825 - traP family protein, at 101,549 to 102,139
_24825
NOLOHH_24830: traB - Protein TraB, at 102,129 to 103,556
traB
Position (kb) 101
102 Strain fitness (log2 ratio) -2
-1
0
1 at 100.574 kb on - strand at 100.574 kb on - strand at 100.598 kb on + strand at 100.626 kb on - strand at 100.677 kb on - strand, within traV at 100.998 kb on - strand, within traV at 101.062 kb on - strand at 101.062 kb on - strand at 101.065 kb on - strand at 101.088 kb on + strand at 101.274 kb on - strand, within trbG at 101.536 kb on - strand, within NOLOHH_24820 at 101.605 kb on - strand at 101.751 kb on + strand, within NOLOHH_24825 at 101.751 kb on + strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.752 kb on - strand, within NOLOHH_24825 at 101.820 kb on + strand, within NOLOHH_24825 at 101.820 kb on + strand, within NOLOHH_24825 at 101.821 kb on - strand, within NOLOHH_24825 at 101.821 kb on - strand, within NOLOHH_24825 at 101.861 kb on + strand, within NOLOHH_24825 at 101.862 kb on - strand, within NOLOHH_24825 at 101.862 kb on - strand, within NOLOHH_24825 at 101.943 kb on - strand, within NOLOHH_24825 at 101.967 kb on - strand, within NOLOHH_24825 at 101.997 kb on - strand, within NOLOHH_24825 at 102.048 kb on + strand, within NOLOHH_24825 at 102.049 kb on - strand, within NOLOHH_24825 at 102.093 kb on + strand at 102.127 kb on + strand at 102.127 kb on + strand at 102.128 kb on - strand at 102.128 kb on - strand at 102.128 kb on - strand at 102.130 kb on + strand at 102.131 kb on - strand at 102.216 kb on - strand at 102.398 kb on - strand, within traB at 102.418 kb on + strand, within traB at 102.418 kb on + strand, within traB at 102.418 kb on + strand, within traB at 102.418 kb on + strand, within traB at 102.419 kb on - strand, within traB at 102.419 kb on - strand, within traB at 102.419 kb on - strand, within traB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 100,574 - +0.9 100,574 - +0.9 100,598 + +0.2 100,626 - +0.8 100,677 - traV NOLOHH_24810 0.17 +0.0 100,998 - traV NOLOHH_24810 0.79 -0.5 101,062 - -0.2 101,062 - -0.3 101,065 - -0.2 101,088 + +1.4 101,274 - trbG NOLOHH_24815 0.68 -0.1 101,536 - NOLOHH_24820 0.86 -0.9 101,605 - +1.6 101,751 + NOLOHH_24825 0.34 -1.3 101,751 + NOLOHH_24825 0.34 +1.1 101,752 - NOLOHH_24825 0.34 -1.2 101,752 - NOLOHH_24825 0.34 -0.5 101,752 - NOLOHH_24825 0.34 +0.4 101,752 - NOLOHH_24825 0.34 +1.2 101,752 - NOLOHH_24825 0.34 +0.4 101,752 - NOLOHH_24825 0.34 +0.5 101,752 - NOLOHH_24825 0.34 -0.6 101,752 - NOLOHH_24825 0.34 +0.2 101,820 + NOLOHH_24825 0.46 +0.4 101,820 + NOLOHH_24825 0.46 +0.3 101,821 - NOLOHH_24825 0.46 -0.7 101,821 - NOLOHH_24825 0.46 -0.4 101,861 + NOLOHH_24825 0.53 +0.5 101,862 - NOLOHH_24825 0.53 +0.2 101,862 - NOLOHH_24825 0.53 +1.0 101,943 - NOLOHH_24825 0.67 +0.8 101,967 - NOLOHH_24825 0.71 +0.2 101,997 - NOLOHH_24825 0.76 -0.4 102,048 + NOLOHH_24825 0.84 -0.1 102,049 - NOLOHH_24825 0.85 -0.6 102,093 + +0.5 102,127 + -0.3 102,127 + -0.1 102,128 - +0.5 102,128 - -0.8 102,128 - -1.1 102,130 + +0.0 102,131 - +0.4 102,216 - -0.4 102,398 - traB NOLOHH_24830 0.19 +0.3 102,418 + traB NOLOHH_24830 0.20 +0.3 102,418 + traB NOLOHH_24830 0.20 +0.2 102,418 + traB NOLOHH_24830 0.20 -0.3 102,418 + traB NOLOHH_24830 0.20 -1.9 102,419 - traB NOLOHH_24830 0.20 -0.5 102,419 - traB NOLOHH_24830 0.20 -0.8 102,419 - traB NOLOHH_24830 0.20 -1.2
Or see this region's nucleotide sequence