Strain Fitness in Escherichia coli ECOR27 around NOLOHH_23250

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolA and tolR are separated by 64 nucleotidestolR and tolQ are separated by 3 nucleotidestolQ and ybgC overlap by 4 nucleotides NOLOHH_23245: tolA - cell envelope integrity protein TolA, at 4,566,919 to 4,568,151 tolA NOLOHH_23250: tolR - colicin uptake protein TolR, at 4,568,216 to 4,568,644 tolR NOLOHH_23255: tolQ - Tol-Pal system protein TolQ, at 4,568,648 to 4,569,340 tolQ NOLOHH_23260: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 4,569,337 to 4,569,741 ybgC Position (kb) 4568 4569Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4567.283 kb on - strand, within tolAat 4567.283 kb on - strand, within tolAat 4567.283 kb on - strand, within tolAat 4567.962 kb on - strand, within tolAat 4567.973 kb on - strand, within tolAat 4568.039 kb on - strandat 4568.039 kb on - strandat 4568.039 kb on - strandat 4568.039 kb on - strandat 4568.041 kb on - strandat 4568.041 kb on - strandat 4568.077 kb on + strandat 4568.104 kb on - strandat 4568.104 kb on - strandat 4568.104 kb on - strandat 4568.218 kb on - strandat 4568.218 kb on - strandat 4568.221 kb on + strandat 4568.261 kb on - strand, within tolRat 4568.261 kb on - strand, within tolRat 4568.261 kb on - strand, within tolRat 4568.265 kb on - strand, within tolRat 4568.296 kb on - strand, within tolRat 4568.425 kb on - strand, within tolRat 4568.435 kb on - strand, within tolRat 4568.574 kb on - strand, within tolRat 4568.588 kb on + strand, within tolRat 4568.724 kb on + strand, within tolQat 4568.765 kb on - strand, within tolQat 4568.766 kb on + strand, within tolQat 4569.002 kb on - strand, within tolQat 4569.361 kb on - strandat 4569.456 kb on + strand, within ybgCat 4569.456 kb on + strand, within ybgCat 4569.509 kb on - strand, within ybgCat 4569.543 kb on - strand, within ybgCat 4569.543 kb on - strand, within ybgCat 4569.561 kb on + strand, within ybgCat 4569.565 kb on + strand, within ybgC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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4,567,283 - tolA NOLOHH_23245 0.30 -2.3
4,567,283 - tolA NOLOHH_23245 0.30 -2.0
4,567,283 - tolA NOLOHH_23245 0.30 -1.6
4,567,962 - tolA NOLOHH_23245 0.85 -0.7
4,567,973 - tolA NOLOHH_23245 0.85 -0.9
4,568,039 - -0.6
4,568,039 - -0.4
4,568,039 - -0.1
4,568,039 - +1.0
4,568,041 - -2.3
4,568,041 - -1.3
4,568,077 + -0.2
4,568,104 - -1.7
4,568,104 - +2.0
4,568,104 - +0.8
4,568,218 - +0.3
4,568,218 - +0.1
4,568,221 + +1.3
4,568,261 - tolR NOLOHH_23250 0.10 -2.3
4,568,261 - tolR NOLOHH_23250 0.10 -0.3
4,568,261 - tolR NOLOHH_23250 0.10 -0.2
4,568,265 - tolR NOLOHH_23250 0.11 +1.3
4,568,296 - tolR NOLOHH_23250 0.19 -1.2
4,568,425 - tolR NOLOHH_23250 0.49 -0.8
4,568,435 - tolR NOLOHH_23250 0.51 -0.7
4,568,574 - tolR NOLOHH_23250 0.83 -1.4
4,568,588 + tolR NOLOHH_23250 0.87 +0.4
4,568,724 + tolQ NOLOHH_23255 0.11 +3.4
4,568,765 - tolQ NOLOHH_23255 0.17 -1.1
4,568,766 + tolQ NOLOHH_23255 0.17 +0.4
4,569,002 - tolQ NOLOHH_23255 0.51 +0.9
4,569,361 - -0.6
4,569,456 + ybgC NOLOHH_23260 0.29 -0.8
4,569,456 + ybgC NOLOHH_23260 0.29 +0.2
4,569,509 - ybgC NOLOHH_23260 0.42 +1.6
4,569,543 - ybgC NOLOHH_23260 0.51 +0.0
4,569,543 - ybgC NOLOHH_23260 0.51 -0.2
4,569,561 + ybgC NOLOHH_23260 0.55 -0.0
4,569,565 + ybgC NOLOHH_23260 0.56 -1.1

Or see this region's nucleotide sequence