Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20745

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnhaB and dsbB are separated by 145 nucleotidesdsbB and umuC are separated by 45 nucleotides NOLOHH_20740: nhaB - Na(+)/H(+) antiporter NhaB, at 4,084,432 to 4,085,973 nhaB NOLOHH_20745: dsbB - disulfide bond formation protein DsbB, at 4,086,119 to 4,086,649 dsbB NOLOHH_20750: umuC - DNA polymerase V catalytic protein, at 4,086,695 to 4,087,963 umuC Position (kb) 4086 4087Strain fitness (log2 ratio) -2 -1 0 1 2at 4085.665 kb on + strand, within nhaBat 4085.872 kb on + strandat 4085.872 kb on + strandat 4085.872 kb on + strandat 4085.992 kb on - strandat 4085.993 kb on + strandat 4085.993 kb on + strandat 4085.993 kb on + strandat 4085.993 kb on + strandat 4085.994 kb on - strandat 4086.022 kb on - strandat 4086.095 kb on + strandat 4086.101 kb on + strandat 4086.102 kb on - strandat 4086.118 kb on + strandat 4086.178 kb on + strand, within dsbBat 4086.254 kb on + strand, within dsbBat 4086.333 kb on + strand, within dsbBat 4086.333 kb on + strand, within dsbBat 4086.333 kb on + strand, within dsbBat 4086.333 kb on + strand, within dsbBat 4086.333 kb on + strand, within dsbBat 4086.353 kb on + strand, within dsbBat 4086.355 kb on + strand, within dsbBat 4086.384 kb on - strand, within dsbBat 4086.575 kb on + strand, within dsbBat 4086.575 kb on + strand, within dsbBat 4086.691 kb on - strandat 4086.697 kb on - strandat 4086.697 kb on - strandat 4086.818 kb on - strandat 4086.885 kb on - strand, within umuCat 4087.057 kb on - strand, within umuCat 4087.310 kb on - strand, within umuC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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4,085,665 + nhaB NOLOHH_20740 0.80 +0.6
4,085,872 + -0.5
4,085,872 + -0.4
4,085,872 + +0.8
4,085,992 - +0.1
4,085,993 + +0.3
4,085,993 + +0.4
4,085,993 + -0.2
4,085,993 + +0.2
4,085,994 - +1.9
4,086,022 - +0.1
4,086,095 + +0.6
4,086,101 + +0.5
4,086,102 - -0.1
4,086,118 + +0.8
4,086,178 + dsbB NOLOHH_20745 0.11 -0.2
4,086,254 + dsbB NOLOHH_20745 0.25 +1.9
4,086,333 + dsbB NOLOHH_20745 0.40 -1.9
4,086,333 + dsbB NOLOHH_20745 0.40 -1.0
4,086,333 + dsbB NOLOHH_20745 0.40 -0.9
4,086,333 + dsbB NOLOHH_20745 0.40 -2.0
4,086,333 + dsbB NOLOHH_20745 0.40 -0.8
4,086,353 + dsbB NOLOHH_20745 0.44 -0.1
4,086,355 + dsbB NOLOHH_20745 0.44 -0.9
4,086,384 - dsbB NOLOHH_20745 0.50 -0.1
4,086,575 + dsbB NOLOHH_20745 0.86 +1.2
4,086,575 + dsbB NOLOHH_20745 0.86 -1.4
4,086,691 - -0.1
4,086,697 - +0.8
4,086,697 - +0.9
4,086,818 - +0.4
4,086,885 - umuC NOLOHH_20750 0.15 -1.1
4,087,057 - umuC NOLOHH_20750 0.29 -1.6
4,087,310 - umuC NOLOHH_20750 0.48 +0.9

Or see this region's nucleotide sequence