Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20715

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnhaP2 and NOLOHH_20715 are separated by 133 nucleotidesNOLOHH_20715 and dadX are separated by 103 nucleotides NOLOHH_20710: nhaP2 - potassium/proton antiporter, at 4,077,076 to 4,078,812 nhaP2 NOLOHH_20715: NOLOHH_20715 - hypothetical protein, at 4,078,946 to 4,079,095 _20715 NOLOHH_20720: dadX - catabolic alanine racemase DadX, at 4,079,199 to 4,080,269 dadX Position (kb) 4078 4079 4080Strain fitness (log2 ratio) -1 0 1at 4078.097 kb on + strand, within nhaP2at 4078.383 kb on + strand, within nhaP2at 4078.401 kb on + strand, within nhaP2at 4078.613 kb on + strand, within nhaP2at 4078.615 kb on + strand, within nhaP2at 4078.615 kb on + strand, within nhaP2at 4078.758 kb on + strandat 4078.981 kb on + strand, within NOLOHH_20715at 4079.065 kb on + strand, within NOLOHH_20715at 4079.124 kb on - strandat 4079.124 kb on - strandat 4079.191 kb on - strandat 4079.499 kb on - strand, within dadXat 4079.501 kb on - strand, within dadXat 4079.584 kb on - strand, within dadXat 4079.640 kb on - strand, within dadXat 4080.093 kb on - strand, within dadX

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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4,078,097 + nhaP2 NOLOHH_20710 0.59 -1.3
4,078,383 + nhaP2 NOLOHH_20710 0.75 +0.2
4,078,401 + nhaP2 NOLOHH_20710 0.76 -1.6
4,078,613 + nhaP2 NOLOHH_20710 0.88 +0.9
4,078,615 + nhaP2 NOLOHH_20710 0.89 +0.6
4,078,615 + nhaP2 NOLOHH_20710 0.89 +0.3
4,078,758 + +0.4
4,078,981 + NOLOHH_20715 0.23 -1.6
4,079,065 + NOLOHH_20715 0.79 -0.6
4,079,124 - +1.1
4,079,124 - +0.4
4,079,191 - +0.5
4,079,499 - dadX NOLOHH_20720 0.28 +0.2
4,079,501 - dadX NOLOHH_20720 0.28 +1.7
4,079,584 - dadX NOLOHH_20720 0.36 +1.1
4,079,640 - dadX NOLOHH_20720 0.41 +0.6
4,080,093 - dadX NOLOHH_20720 0.83 +0.3

Or see this region's nucleotide sequence