Strain Fitness in Escherichia coli ECOR27 around NOLOHH_15585

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntibsA and yegL are separated by 220 nucleotidesyegL and pphC are separated by 77 nucleotidespphC and yegI overlap by 4 nucleotides NOLOHH_15575: ibsA - Small toxic protein IbsA, at 3,061,390 to 3,061,449 ibsA NOLOHH_15580: yegL - Uncharacterized protein YegL, at 3,061,670 to 3,062,329 yegL NOLOHH_15585: pphC - Serine/threonine-protein phosphatase 3, at 3,062,407 to 3,063,087 pphC NOLOHH_15590: yegI - protein kinase YegI, at 3,063,084 to 3,065,024 yegI Position (kb) 3062 3063 3064Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3061.418 kb on + strand, within ibsAat 3061.419 kb on - strand, within ibsAat 3061.424 kb on + strand, within ibsAat 3061.431 kb on + strand, within ibsAat 3061.444 kb on + strandat 3061.444 kb on + strandat 3061.448 kb on - strandat 3061.448 kb on - strandat 3061.448 kb on - strandat 3061.616 kb on + strandat 3061.762 kb on + strand, within yegLat 3061.763 kb on - strand, within yegLat 3061.771 kb on + strand, within yegLat 3061.777 kb on + strand, within yegLat 3061.777 kb on + strand, within yegLat 3061.778 kb on - strand, within yegLat 3061.778 kb on - strand, within yegLat 3061.778 kb on - strand, within yegLat 3061.843 kb on - strand, within yegLat 3061.989 kb on + strand, within yegLat 3061.989 kb on + strand, within yegLat 3062.023 kb on + strand, within yegLat 3062.023 kb on + strand, within yegLat 3062.122 kb on + strand, within yegLat 3062.122 kb on + strand, within yegLat 3062.122 kb on + strand, within yegLat 3062.384 kb on + strandat 3062.385 kb on - strandat 3062.385 kb on - strandat 3062.527 kb on + strand, within pphCat 3062.528 kb on - strand, within pphCat 3062.529 kb on + strand, within pphCat 3062.529 kb on + strand, within pphCat 3062.529 kb on + strand, within pphCat 3062.530 kb on - strand, within pphCat 3063.005 kb on + strand, within pphCat 3063.097 kb on + strandat 3063.098 kb on - strandat 3063.099 kb on + strandat 3063.099 kb on + strandat 3063.100 kb on - strandat 3063.100 kb on - strandat 3063.101 kb on + strandat 3063.101 kb on + strandat 3063.101 kb on + strandat 3063.102 kb on - strandat 3063.102 kb on - strandat 3063.205 kb on - strandat 3063.227 kb on - strandat 3063.283 kb on - strand, within yegIat 3063.283 kb on - strand, within yegIat 3063.283 kb on - strand, within yegIat 3063.341 kb on + strand, within yegIat 3063.378 kb on - strand, within yegIat 3063.385 kb on - strand, within yegIat 3063.442 kb on - strand, within yegIat 3063.529 kb on + strand, within yegIat 3063.530 kb on - strand, within yegIat 3063.601 kb on + strand, within yegIat 3063.602 kb on - strand, within yegIat 3063.745 kb on - strand, within yegI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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3,061,418 + ibsA NOLOHH_15575 0.47 +0.1
3,061,419 - ibsA NOLOHH_15575 0.48 +0.0
3,061,424 + ibsA NOLOHH_15575 0.57 +1.3
3,061,431 + ibsA NOLOHH_15575 0.68 +0.1
3,061,444 + -0.4
3,061,444 + +0.8
3,061,448 - -0.5
3,061,448 - +0.5
3,061,448 - +0.3
3,061,616 + -0.3
3,061,762 + yegL NOLOHH_15580 0.14 -0.3
3,061,763 - yegL NOLOHH_15580 0.14 -0.6
3,061,771 + yegL NOLOHH_15580 0.15 -2.7
3,061,777 + yegL NOLOHH_15580 0.16 -0.2
3,061,777 + yegL NOLOHH_15580 0.16 +1.4
3,061,778 - yegL NOLOHH_15580 0.16 -0.1
3,061,778 - yegL NOLOHH_15580 0.16 +0.2
3,061,778 - yegL NOLOHH_15580 0.16 +0.3
3,061,843 - yegL NOLOHH_15580 0.26 -0.9
3,061,989 + yegL NOLOHH_15580 0.48 +1.5
3,061,989 + yegL NOLOHH_15580 0.48 -0.0
3,062,023 + yegL NOLOHH_15580 0.53 +0.3
3,062,023 + yegL NOLOHH_15580 0.53 +0.5
3,062,122 + yegL NOLOHH_15580 0.68 +1.1
3,062,122 + yegL NOLOHH_15580 0.68 +0.1
3,062,122 + yegL NOLOHH_15580 0.68 +0.4
3,062,384 + -0.8
3,062,385 - +1.4
3,062,385 - +0.3
3,062,527 + pphC NOLOHH_15585 0.18 +0.5
3,062,528 - pphC NOLOHH_15585 0.18 +0.8
3,062,529 + pphC NOLOHH_15585 0.18 -2.5
3,062,529 + pphC NOLOHH_15585 0.18 -0.7
3,062,529 + pphC NOLOHH_15585 0.18 -2.0
3,062,530 - pphC NOLOHH_15585 0.18 +0.1
3,063,005 + pphC NOLOHH_15585 0.88 -1.1
3,063,097 + -1.9
3,063,098 - +0.7
3,063,099 + +1.1
3,063,099 + +0.3
3,063,100 - +0.5
3,063,100 - -1.5
3,063,101 + -1.8
3,063,101 + -1.4
3,063,101 + +2.0
3,063,102 - -0.3
3,063,102 - +1.1
3,063,205 - +0.6
3,063,227 - +0.7
3,063,283 - yegI NOLOHH_15590 0.10 +0.4
3,063,283 - yegI NOLOHH_15590 0.10 +1.8
3,063,283 - yegI NOLOHH_15590 0.10 +1.3
3,063,341 + yegI NOLOHH_15590 0.13 -1.1
3,063,378 - yegI NOLOHH_15590 0.15 -2.1
3,063,385 - yegI NOLOHH_15590 0.16 +0.4
3,063,442 - yegI NOLOHH_15590 0.18 -2.1
3,063,529 + yegI NOLOHH_15590 0.23 -0.6
3,063,530 - yegI NOLOHH_15590 0.23 -0.5
3,063,601 + yegI NOLOHH_15590 0.27 +0.4
3,063,602 - yegI NOLOHH_15590 0.27 -2.9
3,063,745 - yegI NOLOHH_15590 0.34 +1.3

Or see this region's nucleotide sequence