Strain Fitness in Escherichia coli ECOR27 around NOLOHH_13415

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntguaB and guaA are separated by 68 nucleotidesguaA and yfgI are separated by 94 nucleotidesyfgI and yfgH are separated by 15 nucleotides NOLOHH_13410: guaB - IMP dehydrogenase, at 2,603,885 to 2,605,351 guaB NOLOHH_13415: guaA - glutamine-hydrolyzing GMP synthase, at 2,605,420 to 2,606,997 guaA NOLOHH_13420: yfgI - Uncharacterized protein YfgI, at 2,607,092 to 2,607,631 yfgI NOLOHH_13425: yfgH - Uncharacterized lipoprotein YfgH, at 2,607,647 to 2,608,162 yfgH Position (kb) 2605 2606 2607Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2604.793 kb on + strand, within guaBat 2604.822 kb on + strand, within guaBat 2604.822 kb on + strand, within guaBat 2604.822 kb on + strand, within guaBat 2604.822 kb on + strand, within guaBat 2605.151 kb on + strand, within guaBat 2605.151 kb on + strand, within guaBat 2605.151 kb on + strand, within guaBat 2605.153 kb on + strand, within guaBat 2605.228 kb on + strandat 2605.228 kb on + strandat 2605.228 kb on + strandat 2605.263 kb on + strandat 2605.263 kb on + strandat 2605.267 kb on + strandat 2605.315 kb on + strandat 2605.318 kb on + strandat 2605.318 kb on + strandat 2605.334 kb on + strandat 2605.383 kb on + strandat 2605.383 kb on + strandat 2605.383 kb on + strandat 2605.384 kb on - strandat 2605.388 kb on + strandat 2605.388 kb on + strandat 2605.639 kb on + strand, within guaAat 2605.945 kb on + strand, within guaAat 2605.952 kb on + strand, within guaAat 2606.244 kb on + strand, within guaAat 2606.431 kb on + strand, within guaAat 2606.919 kb on + strandat 2606.937 kb on + strandat 2607.036 kb on + strandat 2607.169 kb on + strand, within yfgIat 2607.377 kb on - strand, within yfgIat 2607.394 kb on + strand, within yfgIat 2607.524 kb on - strand, within yfgIat 2607.589 kb on - strandat 2607.649 kb on - strandat 2607.649 kb on - strandat 2607.649 kb on - strandat 2607.671 kb on - strandat 2607.671 kb on - strandat 2607.720 kb on - strand, within yfgHat 2607.764 kb on - strand, within yfgHat 2607.764 kb on - strand, within yfgHat 2607.764 kb on - strand, within yfgHat 2607.765 kb on + strand, within yfgHat 2607.765 kb on + strand, within yfgHat 2607.766 kb on - strand, within yfgHat 2607.766 kb on - strand, within yfgHat 2607.766 kb on - strand, within yfgHat 2607.785 kb on - strand, within yfgHat 2607.785 kb on - strand, within yfgHat 2607.785 kb on - strand, within yfgHat 2607.785 kb on - strand, within yfgHat 2607.830 kb on - strand, within yfgHat 2607.830 kb on - strand, within yfgH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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2,604,793 + guaB NOLOHH_13410 0.62 -0.7
2,604,822 + guaB NOLOHH_13410 0.64 -0.4
2,604,822 + guaB NOLOHH_13410 0.64 -1.3
2,604,822 + guaB NOLOHH_13410 0.64 -1.7
2,604,822 + guaB NOLOHH_13410 0.64 -0.1
2,605,151 + guaB NOLOHH_13410 0.86 -0.4
2,605,151 + guaB NOLOHH_13410 0.86 -0.6
2,605,151 + guaB NOLOHH_13410 0.86 -0.9
2,605,153 + guaB NOLOHH_13410 0.86 -0.6
2,605,228 + +0.9
2,605,228 + -2.5
2,605,228 + -0.4
2,605,263 + -0.1
2,605,263 + -0.5
2,605,267 + -1.7
2,605,315 + +0.5
2,605,318 + +2.5
2,605,318 + +0.0
2,605,334 + -0.4
2,605,383 + -0.3
2,605,383 + +1.0
2,605,383 + -0.4
2,605,384 - -0.8
2,605,388 + -0.3
2,605,388 + -1.3
2,605,639 + guaA NOLOHH_13415 0.14 -2.5
2,605,945 + guaA NOLOHH_13415 0.33 +0.5
2,605,952 + guaA NOLOHH_13415 0.34 -2.2
2,606,244 + guaA NOLOHH_13415 0.52 -1.5
2,606,431 + guaA NOLOHH_13415 0.64 -1.2
2,606,919 + -1.4
2,606,937 + -3.1
2,607,036 + -0.2
2,607,169 + yfgI NOLOHH_13420 0.14 -0.2
2,607,377 - yfgI NOLOHH_13420 0.53 +0.4
2,607,394 + yfgI NOLOHH_13420 0.56 -1.4
2,607,524 - yfgI NOLOHH_13420 0.80 -1.8
2,607,589 - +0.4
2,607,649 - +0.8
2,607,649 - +0.4
2,607,649 - +0.4
2,607,671 - +0.5
2,607,671 - +0.7
2,607,720 - yfgH NOLOHH_13425 0.14 +0.8
2,607,764 - yfgH NOLOHH_13425 0.23 +1.1
2,607,764 - yfgH NOLOHH_13425 0.23 -0.5
2,607,764 - yfgH NOLOHH_13425 0.23 +0.1
2,607,765 + yfgH NOLOHH_13425 0.23 +1.6
2,607,765 + yfgH NOLOHH_13425 0.23 -1.9
2,607,766 - yfgH NOLOHH_13425 0.23 +0.9
2,607,766 - yfgH NOLOHH_13425 0.23 +0.0
2,607,766 - yfgH NOLOHH_13425 0.23 +0.5
2,607,785 - yfgH NOLOHH_13425 0.27 -0.1
2,607,785 - yfgH NOLOHH_13425 0.27 +0.3
2,607,785 - yfgH NOLOHH_13425 0.27 -1.8
2,607,785 - yfgH NOLOHH_13425 0.27 +0.1
2,607,830 - yfgH NOLOHH_13425 0.35 +0.9
2,607,830 - yfgH NOLOHH_13425 0.35 -0.3

Or see this region's nucleotide sequence