Strain Fitness in Escherichia coli ECOR27 around NOLOHH_04525

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthflX and hfq are separated by 75 nucleotideshfq and miaA are separated by 85 nucleotidesmiaA and mutL overlap by 8 nucleotides NOLOHH_04515: hflX - GTPase HflX, at 816,943 to 818,223 hflX NOLOHH_04520: hfq - RNA chaperone Hfq, at 818,299 to 818,607 hfq NOLOHH_04525: miaA - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 818,693 to 819,643 miaA NOLOHH_04530: mutL - DNA mismatch repair endonuclease MutL, at 819,636 to 821,483 mutL Position (kb) 818 819 820Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 817.703 kb on - strand, within hflXat 817.775 kb on - strand, within hflXat 817.897 kb on - strand, within hflXat 818.051 kb on - strand, within hflXat 818.175 kb on - strandat 818.183 kb on - strandat 818.183 kb on - strandat 818.228 kb on - strandat 818.228 kb on - strandat 818.228 kb on - strandat 818.242 kb on - strandat 818.242 kb on - strandat 818.242 kb on - strandat 818.350 kb on - strand, within hfqat 818.361 kb on - strand, within hfqat 818.624 kb on - strandat 818.624 kb on - strandat 818.624 kb on - strandat 818.624 kb on - strandat 818.624 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.630 kb on - strandat 818.636 kb on - strandat 818.636 kb on - strandat 818.636 kb on - strandat 818.674 kb on - strandat 818.674 kb on - strandat 818.724 kb on - strandat 818.796 kb on - strand, within miaAat 819.409 kb on - strand, within miaAat 819.508 kb on + strand, within miaAat 819.551 kb on - strandat 819.628 kb on + strandat 819.670 kb on - strandat 819.685 kb on - strandat 819.723 kb on + strandat 819.724 kb on - strandat 819.724 kb on - strandat 819.724 kb on - strandat 819.724 kb on - strandat 819.815 kb on + strandat 819.815 kb on + strandat 819.816 kb on - strandat 819.856 kb on - strand, within mutLat 819.894 kb on + strand, within mutLat 820.015 kb on - strand, within mutLat 820.083 kb on + strand, within mutLat 820.084 kb on - strand, within mutLat 820.164 kb on - strand, within mutLat 820.271 kb on - strand, within mutLat 820.368 kb on + strand, within mutLat 820.494 kb on + strand, within mutLat 820.516 kb on + strand, within mutLat 820.516 kb on + strand, within mutLat 820.517 kb on - strand, within mutLat 820.517 kb on - strand, within mutLat 820.520 kb on + strand, within mutLat 820.520 kb on + strand, within mutLat 820.521 kb on - strand, within mutLat 820.613 kb on - strand, within mutLat 820.613 kb on - strand, within mutL

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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817,703 - hflX NOLOHH_04515 0.59 +1.3
817,775 - hflX NOLOHH_04515 0.65 -0.4
817,897 - hflX NOLOHH_04515 0.74 -0.4
818,051 - hflX NOLOHH_04515 0.86 +0.6
818,175 - +1.0
818,183 - -0.3
818,183 - +0.4
818,228 - -0.6
818,228 - -0.0
818,228 - -0.7
818,242 - -1.8
818,242 - -0.1
818,242 - -0.1
818,350 - hfq NOLOHH_04520 0.17 -0.1
818,361 - hfq NOLOHH_04520 0.20 -0.5
818,624 - -0.6
818,624 - -2.5
818,624 - -1.3
818,624 - -1.2
818,624 - +0.7
818,630 - -0.2
818,630 - -0.3
818,630 - -1.1
818,630 - +2.7
818,630 - -0.6
818,630 - +0.3
818,630 - +0.2
818,630 - +0.6
818,630 - +0.4
818,630 - -0.9
818,636 - -0.2
818,636 - -0.9
818,636 - -0.4
818,674 - +0.5
818,674 - -0.2
818,724 - -0.5
818,796 - miaA NOLOHH_04525 0.11 -3.3
819,409 - miaA NOLOHH_04525 0.75 -0.9
819,508 + miaA NOLOHH_04525 0.86 -0.7
819,551 - -1.8
819,628 + +0.8
819,670 - -0.7
819,685 - +1.5
819,723 + +0.2
819,724 - -0.4
819,724 - -0.7
819,724 - -0.3
819,724 - +0.8
819,815 + -0.4
819,815 + -0.1
819,816 - +0.0
819,856 - mutL NOLOHH_04530 0.12 -0.3
819,894 + mutL NOLOHH_04530 0.14 -0.5
820,015 - mutL NOLOHH_04530 0.21 -0.3
820,083 + mutL NOLOHH_04530 0.24 +0.2
820,084 - mutL NOLOHH_04530 0.24 +0.1
820,164 - mutL NOLOHH_04530 0.29 +0.2
820,271 - mutL NOLOHH_04530 0.34 +1.7
820,368 + mutL NOLOHH_04530 0.40 +0.2
820,494 + mutL NOLOHH_04530 0.46 -0.3
820,516 + mutL NOLOHH_04530 0.48 +0.4
820,516 + mutL NOLOHH_04530 0.48 -0.5
820,517 - mutL NOLOHH_04530 0.48 +0.5
820,517 - mutL NOLOHH_04530 0.48 -0.2
820,520 + mutL NOLOHH_04530 0.48 -0.3
820,520 + mutL NOLOHH_04530 0.48 +0.2
820,521 - mutL NOLOHH_04530 0.48 -0.4
820,613 - mutL NOLOHH_04530 0.53 -0.7
820,613 - mutL NOLOHH_04530 0.53 +0.1

Or see this region's nucleotide sequence