Strain Fitness in Escherichia coli ECOR27 around NOLOHH_03300

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntispH and fkpB are separated by 1 nucleotidesfkpB and lspA are separated by 87 nucleotideslspA and ileS overlap by 1 nucleotides NOLOHH_03295: ispH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 580,498 to 581,448 ispH NOLOHH_03300: fkpB - FKBP-type peptidyl-prolyl cis-trans isomerase, at 581,450 to 581,899 fkpB NOLOHH_03305: lspA - signal peptidase II, at 581,987 to 582,481 lspA NOLOHH_03310: ileS - isoleucine--tRNA ligase, at 582,481 to 585,297 ileS Position (kb) 581 582Strain fitness (log2 ratio) -1 0 1at 580.492 kb on - strandat 581.723 kb on - strand, within fkpBat 581.914 kb on - strandat 581.914 kb on - strandat 581.986 kb on - strandat 581.986 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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580,492 - -1.1
581,723 - fkpB NOLOHH_03300 0.61 -0.8
581,914 - +0.2
581,914 - +0.6
581,986 - -0.5
581,986 - +0.8

Or see this region's nucleotide sequence