Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speD and yacL are separated by 37 nucleotides yacL and acnB are separated by 174 nucleotides acnB and NOLOHH_02830 are separated by 354 nucleotides
NOLOHH_02815: speD - adenosylmethionine decarboxylase, at 473,861 to 474,655
speD
NOLOHH_02820: yacL - protein YacL, at 474,693 to 475,055
yacL
NOLOHH_02825: acnB - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 475,230 to 477,827
acnB
NOLOHH_02830: NOLOHH_02830 - DUF3300 domain-containing protein, at 478,182 to 479,939
_02830
Position (kb) 475
476
477
478 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 474.247 kb on + strand, within speD at 474.311 kb on + strand, within speD at 474.342 kb on + strand, within speD at 474.627 kb on - strand at 474.641 kb on - strand at 474.695 kb on - strand at 474.778 kb on + strand, within yacL at 474.779 kb on - strand, within yacL at 474.779 kb on - strand, within yacL at 474.779 kb on - strand, within yacL at 474.782 kb on - strand, within yacL at 474.868 kb on + strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 475.007 kb on + strand, within yacL at 475.007 kb on + strand, within yacL at 475.007 kb on + strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.017 kb on + strand, within yacL at 475.018 kb on - strand, within yacL at 475.055 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.156 kb on + strand at 475.224 kb on + strand at 475.228 kb on + strand at 475.229 kb on - strand at 475.229 kb on - strand at 475.229 kb on - strand at 475.232 kb on - strand at 475.274 kb on - strand at 475.310 kb on + strand at 475.311 kb on - strand at 475.326 kb on - strand at 475.326 kb on - strand at 475.568 kb on - strand, within acnB at 475.647 kb on - strand, within acnB at 476.268 kb on + strand, within acnB at 476.436 kb on - strand, within acnB at 477.048 kb on - strand, within acnB at 477.433 kb on - strand, within acnB at 477.433 kb on - strand, within acnB at 477.489 kb on - strand, within acnB at 477.855 kb on - strand at 477.907 kb on - strand at 478.103 kb on - strand at 478.113 kb on + strand at 478.113 kb on + strand at 478.113 kb on + strand at 478.114 kb on - strand at 478.114 kb on - strand at 478.114 kb on - strand at 478.115 kb on + strand at 478.222 kb on + strand at 478.261 kb on + strand at 478.261 kb on + strand at 478.261 kb on + strand at 478.261 kb on + strand at 478.261 kb on + strand at 478.262 kb on - strand at 478.263 kb on + strand at 478.263 kb on + strand at 478.263 kb on + strand at 478.263 kb on + strand at 478.263 kb on + strand at 478.264 kb on - strand at 478.264 kb on - strand at 478.264 kb on - strand at 478.264 kb on - strand at 478.264 kb on - strand at 478.264 kb on - strand at 478.265 kb on + strand at 478.265 kb on + strand at 478.266 kb on - strand at 478.266 kb on - strand at 478.267 kb on + strand at 478.268 kb on - strand at 478.341 kb on - strand at 478.444 kb on - strand, within NOLOHH_02830 at 478.486 kb on - strand, within NOLOHH_02830 at 478.565 kb on - strand, within NOLOHH_02830 at 478.592 kb on + strand, within NOLOHH_02830 at 478.770 kb on - strand, within NOLOHH_02830 at 478.772 kb on + strand, within NOLOHH_02830 at 478.773 kb on - strand, within NOLOHH_02830
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 474,247 + speD NOLOHH_02815 0.49 +0.4 474,311 + speD NOLOHH_02815 0.57 -0.2 474,342 + speD NOLOHH_02815 0.61 +0.2 474,627 - -2.6 474,641 - +0.1 474,695 - +0.1 474,778 + yacL NOLOHH_02820 0.23 +1.7 474,779 - yacL NOLOHH_02820 0.24 +0.1 474,779 - yacL NOLOHH_02820 0.24 -0.2 474,779 - yacL NOLOHH_02820 0.24 -1.2 474,782 - yacL NOLOHH_02820 0.25 -2.6 474,868 + yacL NOLOHH_02820 0.48 +0.2 474,869 - yacL NOLOHH_02820 0.48 +0.4 474,869 - yacL NOLOHH_02820 0.48 +0.2 474,869 - yacL NOLOHH_02820 0.48 +0.6 474,869 - yacL NOLOHH_02820 0.48 +0.9 474,869 - yacL NOLOHH_02820 0.48 -3.4 474,869 - yacL NOLOHH_02820 0.48 +0.3 474,869 - yacL NOLOHH_02820 0.48 -2.9 474,869 - yacL NOLOHH_02820 0.48 +0.4 474,869 - yacL NOLOHH_02820 0.48 -0.4 474,869 - yacL NOLOHH_02820 0.48 -0.2 474,869 - yacL NOLOHH_02820 0.48 +0.6 475,007 + yacL NOLOHH_02820 0.87 -0.5 475,007 + yacL NOLOHH_02820 0.87 +0.2 475,007 + yacL NOLOHH_02820 0.87 -1.6 475,008 - yacL NOLOHH_02820 0.87 -0.5 475,008 - yacL NOLOHH_02820 0.87 +0.5 475,008 - yacL NOLOHH_02820 0.87 +0.0 475,008 - yacL NOLOHH_02820 0.87 +0.8 475,008 - yacL NOLOHH_02820 0.87 -1.3 475,017 + yacL NOLOHH_02820 0.89 +0.0 475,018 - yacL NOLOHH_02820 0.90 +0.2 475,055 + +0.4 475,109 + +0.7 475,109 + +0.5 475,109 + +1.2 475,109 + +0.5 475,109 + -0.5 475,110 - +0.1 475,110 - +0.3 475,110 - +0.4 475,110 - -0.1 475,110 - -1.8 475,156 + +0.2 475,224 + -0.6 475,228 + -0.1 475,229 - +1.8 475,229 - -0.2 475,229 - -0.6 475,232 - +0.2 475,274 - +0.1 475,310 + +2.2 475,311 - -0.0 475,326 - -0.8 475,326 - -0.9 475,568 - acnB NOLOHH_02825 0.13 -0.4 475,647 - acnB NOLOHH_02825 0.16 +0.2 476,268 + acnB NOLOHH_02825 0.40 +0.4 476,436 - acnB NOLOHH_02825 0.46 -0.6 477,048 - acnB NOLOHH_02825 0.70 -1.0 477,433 - acnB NOLOHH_02825 0.85 +0.6 477,433 - acnB NOLOHH_02825 0.85 +0.2 477,489 - acnB NOLOHH_02825 0.87 +1.2 477,855 - +0.5 477,907 - -1.3 478,103 - +1.3 478,113 + +1.1 478,113 + +0.3 478,113 + -0.3 478,114 - +0.4 478,114 - -0.1 478,114 - -0.7 478,115 + -0.3 478,222 + +0.3 478,261 + -0.2 478,261 + +0.2 478,261 + +0.0 478,261 + +0.9 478,261 + +0.0 478,262 - -0.2 478,263 + +2.0 478,263 + +1.9 478,263 + +2.0 478,263 + +0.4 478,263 + +0.4 478,264 - +0.3 478,264 - +0.0 478,264 - +0.8 478,264 - -0.5 478,264 - -0.6 478,264 - -1.2 478,265 + -0.0 478,265 + +0.3 478,266 - +0.4 478,266 - +0.3 478,267 + +0.9 478,268 - -0.3 478,341 - -0.3 478,444 - NOLOHH_02830 0.15 +0.2 478,486 - NOLOHH_02830 0.17 +0.8 478,565 - NOLOHH_02830 0.22 -0.6 478,592 + NOLOHH_02830 0.23 -0.7 478,770 - NOLOHH_02830 0.33 -1.5 478,772 + NOLOHH_02830 0.34 +0.2 478,773 - NOLOHH_02830 0.34 -0.3
Or see this region's nucleotide sequence