Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27770

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdsA and sirB1 are separated by 35 nucleotidessirB1 and sirB2 are separated by 3 nucleotidessirB2 and prmC overlap by 4 nucleotides NIAGMN_27765: kdsA - 3-deoxy-8-phosphooctulonate synthase, at 5,378,889 to 5,379,743 kdsA NIAGMN_27770: sirB1 - invasion regulator SirB1, at 5,379,779 to 5,380,588 sirB1 NIAGMN_27775: sirB2 - invasion regulator SirB2, at 5,380,592 to 5,380,984 sirB2 NIAGMN_27780: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 5,380,981 to 5,381,814 prmC Position (kb) 5379 5380 5381Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5379.794 kb on - strandat 5379.794 kb on - strandat 5379.871 kb on - strand, within sirB1at 5379.876 kb on - strand, within sirB1at 5379.876 kb on - strand, within sirB1at 5380.086 kb on + strand, within sirB1at 5380.139 kb on - strand, within sirB1at 5380.171 kb on + strand, within sirB1at 5380.559 kb on - strandat 5380.694 kb on - strand, within sirB2at 5380.732 kb on - strand, within sirB2at 5380.902 kb on - strand, within sirB2at 5380.961 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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5,379,794 - +1.6
5,379,794 - +0.7
5,379,871 - sirB1 NIAGMN_27770 0.11 +1.0
5,379,876 - sirB1 NIAGMN_27770 0.12 +1.2
5,379,876 - sirB1 NIAGMN_27770 0.12 +1.1
5,380,086 + sirB1 NIAGMN_27770 0.38 -1.2
5,380,139 - sirB1 NIAGMN_27770 0.44 -2.8
5,380,171 + sirB1 NIAGMN_27770 0.48 +0.6
5,380,559 - +0.1
5,380,694 - sirB2 NIAGMN_27775 0.26 +1.9
5,380,732 - sirB2 NIAGMN_27775 0.36 +0.5
5,380,902 - sirB2 NIAGMN_27775 0.79 +0.2
5,380,961 - -1.7

Or see this region's nucleotide sequence