Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolA and tolR are separated by 64 nucleotides tolR and tolQ are separated by 3 nucleotides tolQ and ybgC overlap by 4 nucleotides ybgC and ybgE are separated by 149 nucleotides ybgE and cydX overlap by 1 nucleotides cydX and cydB are separated by 14 nucleotides
NOLOHH_23245: tolA - cell envelope integrity protein TolA, at 4,566,919 to 4,568,151
tolA
NOLOHH_23250: tolR - colicin uptake protein TolR, at 4,568,216 to 4,568,644
tolR
NOLOHH_23255: tolQ - Tol-Pal system protein TolQ, at 4,568,648 to 4,569,340
tolQ
NOLOHH_23260: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 4,569,337 to 4,569,741
ybgC
NOLOHH_23265: ybgE - cyd operon protein YbgE, at 4,569,891 to 4,570,184
ybgE
NOLOHH_23270: cydX - cytochrome bd-I oxidase subunit CydX, at 4,570,184 to 4,570,297
cydX
NOLOHH_23275: cydB - cytochrome d ubiquinol oxidase subunit II, at 4,570,312 to 4,571,451
cydB
Position (kb) 4568
4569
4570 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4567.962 kb on - strand, within tolA at 4567.973 kb on - strand, within tolA at 4568.039 kb on - strand at 4568.039 kb on - strand at 4568.039 kb on - strand at 4568.039 kb on - strand at 4568.041 kb on - strand at 4568.041 kb on - strand at 4568.077 kb on + strand at 4568.104 kb on - strand at 4568.104 kb on - strand at 4568.104 kb on - strand at 4568.218 kb on - strand at 4568.218 kb on - strand at 4568.221 kb on + strand at 4568.261 kb on - strand, within tolR at 4568.261 kb on - strand, within tolR at 4568.261 kb on - strand, within tolR at 4568.265 kb on - strand, within tolR at 4568.296 kb on - strand, within tolR at 4568.425 kb on - strand, within tolR at 4568.435 kb on - strand, within tolR at 4568.574 kb on - strand, within tolR at 4568.588 kb on + strand, within tolR at 4568.724 kb on + strand, within tolQ at 4568.765 kb on - strand, within tolQ at 4568.766 kb on + strand, within tolQ at 4569.002 kb on - strand, within tolQ at 4569.361 kb on - strand at 4569.456 kb on + strand, within ybgC at 4569.456 kb on + strand, within ybgC at 4569.509 kb on - strand, within ybgC at 4569.543 kb on - strand, within ybgC at 4569.543 kb on - strand, within ybgC at 4569.561 kb on + strand, within ybgC at 4569.565 kb on + strand, within ybgC at 4569.647 kb on + strand, within ybgC at 4569.659 kb on + strand, within ybgC at 4569.659 kb on + strand, within ybgC at 4569.735 kb on + strand at 4569.736 kb on - strand at 4569.744 kb on + strand at 4569.745 kb on - strand at 4569.745 kb on - strand at 4569.745 kb on - strand at 4569.779 kb on - strand at 4569.788 kb on + strand at 4569.789 kb on - strand at 4569.791 kb on + strand at 4569.791 kb on + strand at 4569.792 kb on - strand at 4569.794 kb on - strand at 4570.091 kb on + strand, within ybgE at 4570.092 kb on - strand, within ybgE at 4570.092 kb on - strand, within ybgE at 4570.310 kb on - strand at 4570.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 4,567,962 - tolA NOLOHH_23245 0.85 -1.0 4,567,973 - tolA NOLOHH_23245 0.85 -1.5 4,568,039 - +2.4 4,568,039 - +1.0 4,568,039 - -0.7 4,568,039 - -1.0 4,568,041 - -0.6 4,568,041 - -1.7 4,568,077 + -1.1 4,568,104 - +0.6 4,568,104 - +2.3 4,568,104 - +0.4 4,568,218 - -1.0 4,568,218 - +0.4 4,568,221 + -2.2 4,568,261 - tolR NOLOHH_23250 0.10 -1.6 4,568,261 - tolR NOLOHH_23250 0.10 +0.3 4,568,261 - tolR NOLOHH_23250 0.10 +0.9 4,568,265 - tolR NOLOHH_23250 0.11 +0.3 4,568,296 - tolR NOLOHH_23250 0.19 -0.5 4,568,425 - tolR NOLOHH_23250 0.49 +0.2 4,568,435 - tolR NOLOHH_23250 0.51 -0.0 4,568,574 - tolR NOLOHH_23250 0.83 -1.3 4,568,588 + tolR NOLOHH_23250 0.87 +1.1 4,568,724 + tolQ NOLOHH_23255 0.11 +1.1 4,568,765 - tolQ NOLOHH_23255 0.17 -1.0 4,568,766 + tolQ NOLOHH_23255 0.17 +0.1 4,569,002 - tolQ NOLOHH_23255 0.51 -0.4 4,569,361 - -1.3 4,569,456 + ybgC NOLOHH_23260 0.29 +1.4 4,569,456 + ybgC NOLOHH_23260 0.29 -1.1 4,569,509 - ybgC NOLOHH_23260 0.42 -0.1 4,569,543 - ybgC NOLOHH_23260 0.51 -0.3 4,569,543 - ybgC NOLOHH_23260 0.51 -0.5 4,569,561 + ybgC NOLOHH_23260 0.55 -0.3 4,569,565 + ybgC NOLOHH_23260 0.56 -1.0 4,569,647 + ybgC NOLOHH_23260 0.77 -0.4 4,569,659 + ybgC NOLOHH_23260 0.80 -1.2 4,569,659 + ybgC NOLOHH_23260 0.80 -0.1 4,569,735 + -0.2 4,569,736 - -0.5 4,569,744 + +0.0 4,569,745 - -0.5 4,569,745 - +0.4 4,569,745 - -0.9 4,569,779 - -0.2 4,569,788 + -0.2 4,569,789 - +0.7 4,569,791 + +0.6 4,569,791 + -1.5 4,569,792 - +0.7 4,569,794 - +1.3 4,570,091 + ybgE NOLOHH_23265 0.68 +1.0 4,570,092 - ybgE NOLOHH_23265 0.68 +0.3 4,570,092 - ybgE NOLOHH_23265 0.68 -1.0 4,570,310 - -2.5 4,570,314 - +0.1
Or see this region's nucleotide sequence