Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflB and pflA are separated by 191 nucleotides pflA and mdaB are separated by 81 nucleotides mdaB and ycaN are separated by 99 nucleotides
NOLOHH_22070: pflB - formate C-acetyltransferase, at 4,349,417 to 4,351,699
pflB
NOLOHH_22075: pflA - pyruvate formate lyase 1-activating protein, at 4,351,891 to 4,352,631
pflA
NOLOHH_22080: mdaB - NAD(P)H oxidoreductase, at 4,352,713 to 4,353,303
mdaB
NOLOHH_22085: ycaN - Uncharacterized HTH-type transcriptional regulator YcaN, at 4,353,403 to 4,354,311
ycaN
Position (kb)
4351
4352
4353 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4350.935 kb on + strand, within pflB at 4351.137 kb on + strand, within pflB at 4351.137 kb on + strand, within pflB at 4351.199 kb on + strand, within pflB at 4351.199 kb on + strand, within pflB at 4351.253 kb on + strand, within pflB at 4351.412 kb on + strand, within pflB at 4351.614 kb on + strand at 4351.649 kb on - strand at 4351.672 kb on + strand at 4351.672 kb on + strand at 4351.678 kb on + strand at 4351.693 kb on + strand at 4351.694 kb on - strand at 4351.697 kb on + strand at 4351.738 kb on + strand at 4351.793 kb on - strand at 4351.796 kb on + strand at 4351.797 kb on - strand at 4351.798 kb on + strand at 4351.799 kb on - strand at 4351.832 kb on - strand at 4351.832 kb on - strand at 4351.951 kb on - strand at 4352.089 kb on - strand, within pflA at 4352.138 kb on + strand, within pflA at 4352.193 kb on + strand, within pflA at 4352.356 kb on + strand, within pflA at 4352.565 kb on + strand at 4352.616 kb on + strand at 4352.621 kb on + strand at 4352.726 kb on - strand at 4352.731 kb on - strand at 4352.750 kb on - strand at 4352.760 kb on - strand at 4352.803 kb on - strand, within mdaB at 4352.803 kb on - strand, within mdaB at 4352.833 kb on - strand, within mdaB at 4352.851 kb on - strand, within mdaB at 4352.950 kb on + strand, within mdaB at 4352.983 kb on - strand, within mdaB at 4353.001 kb on - strand, within mdaB at 4353.001 kb on - strand, within mdaB at 4353.003 kb on - strand, within mdaB at 4353.011 kb on - strand, within mdaB at 4353.084 kb on - strand, within mdaB at 4353.084 kb on - strand, within mdaB at 4353.179 kb on - strand, within mdaB at 4353.284 kb on + strand at 4353.285 kb on - strand at 4353.384 kb on + strand at 4353.414 kb on + strand at 4353.415 kb on - strand at 4353.483 kb on - strand at 4353.516 kb on + strand, within ycaN
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 4,350,935 + pflB NOLOHH_22070 0.66 -0.3 4,351,137 + pflB NOLOHH_22070 0.75 -1.4 4,351,137 + pflB NOLOHH_22070 0.75 +0.7 4,351,199 + pflB NOLOHH_22070 0.78 +1.0 4,351,199 + pflB NOLOHH_22070 0.78 +0.6 4,351,253 + pflB NOLOHH_22070 0.80 +0.9 4,351,412 + pflB NOLOHH_22070 0.87 +0.5 4,351,614 + -1.6 4,351,649 - +2.6 4,351,672 + -0.3 4,351,672 + +0.3 4,351,678 + -1.4 4,351,693 + +0.3 4,351,694 - +0.5 4,351,697 + -0.6 4,351,738 + -0.1 4,351,793 - +1.4 4,351,796 + +0.1 4,351,797 - +0.6 4,351,798 + +1.5 4,351,799 - -1.2 4,351,832 - -0.6 4,351,832 - -0.6 4,351,951 - +0.7 4,352,089 - pflA NOLOHH_22075 0.27 -2.9 4,352,138 + pflA NOLOHH_22075 0.33 +0.1 4,352,193 + pflA NOLOHH_22075 0.41 +0.2 4,352,356 + pflA NOLOHH_22075 0.63 -1.4 4,352,565 + -0.2 4,352,616 + +0.3 4,352,621 + +1.6 4,352,726 - -0.3 4,352,731 - +0.4 4,352,750 - -1.7 4,352,760 - +0.5 4,352,803 - mdaB NOLOHH_22080 0.15 -1.2 4,352,803 - mdaB NOLOHH_22080 0.15 +0.0 4,352,833 - mdaB NOLOHH_22080 0.20 +0.1 4,352,851 - mdaB NOLOHH_22080 0.23 +1.6 4,352,950 + mdaB NOLOHH_22080 0.40 +0.2 4,352,983 - mdaB NOLOHH_22080 0.46 -0.6 4,353,001 - mdaB NOLOHH_22080 0.49 -2.5 4,353,001 - mdaB NOLOHH_22080 0.49 +0.4 4,353,003 - mdaB NOLOHH_22080 0.49 +0.6 4,353,011 - mdaB NOLOHH_22080 0.50 -0.0 4,353,084 - mdaB NOLOHH_22080 0.63 +1.1 4,353,084 - mdaB NOLOHH_22080 0.63 +0.3 4,353,179 - mdaB NOLOHH_22080 0.79 +1.1 4,353,284 + +3.0 4,353,285 - -1.6 4,353,384 + +1.1 4,353,414 + -0.4 4,353,415 - +1.6 4,353,483 - +1.1 4,353,516 + ycaN NOLOHH_22085 0.12 -0.1
Or see this region's nucleotide sequence