Strain Fitness in Escherichia coli ECOR27 around NOLOHH_21380

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcgsD and cgsE are separated by 4 nucleotidescgsE and cgsF are separated by 24 nucleotidescgsF and cgsG are separated by 26 nucleotides NOLOHH_21375: cgsD - transcriptional regulator CsgD, at 4,200,856 to 4,201,506 cgsD NOLOHH_21380: cgsE - curli production assembly/transport protein CsgE, at 4,201,511 to 4,201,900 cgsE NOLOHH_21385: cgsF - curli production assembly/transport protein CsgF, at 4,201,925 to 4,202,341 cgsF NOLOHH_21390: cgsG - curli production assembly/transport protein CsgG, at 4,202,368 to 4,203,201 cgsG Position (kb) 4201 4202Strain fitness (log2 ratio) -2 -1 0 1at 4200.690 kb on + strandat 4200.714 kb on + strandat 4200.743 kb on + strandat 4200.754 kb on + strandat 4201.299 kb on + strand, within cgsDat 4201.524 kb on + strandat 4201.851 kb on + strand, within cgsEat 4201.960 kb on + strandat 4202.574 kb on + strand, within cgsGat 4202.575 kb on - strand, within cgsGat 4202.772 kb on + strand, within cgsGat 4202.772 kb on + strand, within cgsG

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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4,200,690 + +0.2
4,200,714 + -0.3
4,200,743 + -0.4
4,200,754 + -2.0
4,201,299 + cgsD NOLOHH_21375 0.68 -0.2
4,201,524 + +1.0
4,201,851 + cgsE NOLOHH_21380 0.87 -0.9
4,201,960 + +0.1
4,202,574 + cgsG NOLOHH_21390 0.25 -0.5
4,202,575 - cgsG NOLOHH_21390 0.25 +0.7
4,202,772 + cgsG NOLOHH_21390 0.48 +0.1
4,202,772 + cgsG NOLOHH_21390 0.48 -0.9

Or see this region's nucleotide sequence