Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgD and flgC are separated by 11 nucleotides flgC and flgB are separated by 3 nucleotides flgB and flgA are separated by 154 nucleotides flgA and flgM are separated by 75 nucleotides
NOLOHH_21195: flgD - flagellar hook assembly protein FlgD, at 4,171,501 to 4,172,196
flgD
NOLOHH_21200: flgC - flagellar basal body rod protein FlgC, at 4,172,208 to 4,172,612
flgC
NOLOHH_21205: flgB - flagellar basal body rod protein FlgB, at 4,172,616 to 4,173,032
flgB
NOLOHH_21210: flgA - flagellar basal body P-ring formation protein FlgA, at 4,173,187 to 4,173,846
flgA
NOLOHH_21215: flgM - anti-sigma-28 factor FlgM, at 4,173,922 to 4,174,215
flgM
Position (kb)
4172
4173
4174 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4171.630 kb on - strand, within flgD at 4171.654 kb on + strand, within flgD at 4171.661 kb on + strand, within flgD at 4171.661 kb on + strand, within flgD at 4171.661 kb on + strand, within flgD at 4171.661 kb on + strand, within flgD at 4171.662 kb on - strand, within flgD at 4171.662 kb on - strand, within flgD at 4171.928 kb on - strand, within flgD at 4171.942 kb on - strand, within flgD at 4172.105 kb on - strand, within flgD at 4172.136 kb on + strand at 4172.137 kb on - strand at 4172.160 kb on + strand at 4172.196 kb on + strand at 4172.197 kb on - strand at 4172.197 kb on - strand at 4172.381 kb on - strand, within flgC at 4172.410 kb on - strand, within flgC at 4172.428 kb on - strand, within flgC at 4172.632 kb on - strand at 4172.769 kb on - strand, within flgB at 4173.032 kb on + strand at 4173.033 kb on - strand at 4173.033 kb on - strand at 4173.070 kb on - strand at 4173.102 kb on - strand at 4173.120 kb on + strand at 4173.124 kb on + strand at 4173.125 kb on - strand at 4173.153 kb on - strand at 4173.330 kb on + strand, within flgA at 4173.344 kb on + strand, within flgA at 4173.476 kb on - strand, within flgA at 4173.476 kb on - strand, within flgA at 4173.620 kb on + strand, within flgA at 4173.798 kb on - strand at 4173.836 kb on + strand at 4173.880 kb on + strand at 4173.880 kb on + strand at 4173.880 kb on + strand at 4173.893 kb on + strand at 4173.895 kb on - strand at 4173.927 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 4,171,630 - flgD NOLOHH_21195 0.19 +0.4 4,171,654 + flgD NOLOHH_21195 0.22 -0.6 4,171,661 + flgD NOLOHH_21195 0.23 -1.3 4,171,661 + flgD NOLOHH_21195 0.23 -0.1 4,171,661 + flgD NOLOHH_21195 0.23 -0.9 4,171,661 + flgD NOLOHH_21195 0.23 -2.3 4,171,662 - flgD NOLOHH_21195 0.23 -1.5 4,171,662 - flgD NOLOHH_21195 0.23 -1.1 4,171,928 - flgD NOLOHH_21195 0.61 +0.1 4,171,942 - flgD NOLOHH_21195 0.63 -0.1 4,172,105 - flgD NOLOHH_21195 0.87 -0.6 4,172,136 + +0.1 4,172,137 - -0.9 4,172,160 + -0.8 4,172,196 + -0.4 4,172,197 - -0.2 4,172,197 - +0.5 4,172,381 - flgC NOLOHH_21200 0.43 -1.6 4,172,410 - flgC NOLOHH_21200 0.50 -0.7 4,172,428 - flgC NOLOHH_21200 0.54 +0.3 4,172,632 - -0.1 4,172,769 - flgB NOLOHH_21205 0.37 -0.9 4,173,032 + -1.1 4,173,033 - -0.4 4,173,033 - -0.2 4,173,070 - +1.0 4,173,102 - -0.5 4,173,120 + +1.1 4,173,124 + +0.5 4,173,125 - +0.8 4,173,153 - +1.9 4,173,330 + flgA NOLOHH_21210 0.22 +0.5 4,173,344 + flgA NOLOHH_21210 0.24 -1.1 4,173,476 - flgA NOLOHH_21210 0.44 -0.1 4,173,476 - flgA NOLOHH_21210 0.44 +0.1 4,173,620 + flgA NOLOHH_21210 0.66 +0.6 4,173,798 - -1.0 4,173,836 + +0.6 4,173,880 + +0.5 4,173,880 + -1.8 4,173,880 + +0.7 4,173,893 + -0.2 4,173,895 - -0.9 4,173,927 + +0.7
Or see this region's nucleotide sequence