Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20710

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntldcA and nhaP2 are separated by 94 nucleotidesnhaP2 and NOLOHH_20715 are separated by 133 nucleotidesNOLOHH_20715 and dadX are separated by 103 nucleotides NOLOHH_20705: ldcA - muramoyltetrapeptide carboxypeptidase, at 4,076,067 to 4,076,981 ldcA NOLOHH_20710: nhaP2 - potassium/proton antiporter, at 4,077,076 to 4,078,812 nhaP2 NOLOHH_20715: NOLOHH_20715 - hypothetical protein, at 4,078,946 to 4,079,095 _20715 NOLOHH_20720: dadX - catabolic alanine racemase DadX, at 4,079,199 to 4,080,269 dadX Position (kb) 4077 4078 4079Strain fitness (log2 ratio) -1 0 1 2 3at 4076.083 kb on + strandat 4076.109 kb on - strandat 4077.005 kb on + strandat 4077.028 kb on + strandat 4077.040 kb on + strandat 4077.040 kb on + strandat 4077.041 kb on - strandat 4077.043 kb on - strandat 4077.056 kb on + strandat 4077.144 kb on + strandat 4077.144 kb on + strandat 4077.266 kb on + strand, within nhaP2at 4077.266 kb on + strand, within nhaP2at 4077.270 kb on + strand, within nhaP2at 4077.270 kb on + strand, within nhaP2at 4077.694 kb on + strand, within nhaP2at 4078.097 kb on + strand, within nhaP2at 4078.383 kb on + strand, within nhaP2at 4078.401 kb on + strand, within nhaP2at 4078.613 kb on + strand, within nhaP2at 4078.615 kb on + strand, within nhaP2at 4078.615 kb on + strand, within nhaP2at 4078.758 kb on + strandat 4078.981 kb on + strand, within NOLOHH_20715at 4079.065 kb on + strand, within NOLOHH_20715at 4079.124 kb on - strandat 4079.124 kb on - strandat 4079.191 kb on - strandat 4079.499 kb on - strand, within dadXat 4079.501 kb on - strand, within dadXat 4079.584 kb on - strand, within dadXat 4079.640 kb on - strand, within dadX

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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4,076,083 + +1.1
4,076,109 - +0.7
4,077,005 + -0.6
4,077,028 + +3.0
4,077,040 + +0.5
4,077,040 + -0.1
4,077,041 - +1.1
4,077,043 - -1.0
4,077,056 + +0.9
4,077,144 + -0.1
4,077,144 + +1.9
4,077,266 + nhaP2 NOLOHH_20710 0.11 -0.4
4,077,266 + nhaP2 NOLOHH_20710 0.11 -1.5
4,077,270 + nhaP2 NOLOHH_20710 0.11 +1.1
4,077,270 + nhaP2 NOLOHH_20710 0.11 -0.1
4,077,694 + nhaP2 NOLOHH_20710 0.36 -0.2
4,078,097 + nhaP2 NOLOHH_20710 0.59 -0.5
4,078,383 + nhaP2 NOLOHH_20710 0.75 +0.7
4,078,401 + nhaP2 NOLOHH_20710 0.76 -0.4
4,078,613 + nhaP2 NOLOHH_20710 0.88 -0.3
4,078,615 + nhaP2 NOLOHH_20710 0.89 +0.3
4,078,615 + nhaP2 NOLOHH_20710 0.89 -0.5
4,078,758 + +0.5
4,078,981 + NOLOHH_20715 0.23 -0.6
4,079,065 + NOLOHH_20715 0.79 -0.4
4,079,124 - -0.3
4,079,124 - -1.5
4,079,191 - +0.7
4,079,499 - dadX NOLOHH_20720 0.28 +0.6
4,079,501 - dadX NOLOHH_20720 0.28 +0.9
4,079,584 - dadX NOLOHH_20720 0.36 +0.5
4,079,640 - dadX NOLOHH_20720 0.41 +1.1

Or see this region's nucleotide sequence