Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20380

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciA and tonB are separated by 39 nucleotidestonB and yciI are separated by 223 nucleotidesyciI and kch are separated by 298 nucleotides NOLOHH_20370: yciA - acyl-CoA thioester hydrolase YciA, at 4,009,558 to 4,009,956 yciA NOLOHH_20375: tonB - TonB system transport protein TonB, at 4,009,996 to 4,010,715 tonB NOLOHH_20380: yciI - YciI family protein, at 4,010,939 to 4,011,235 yciI NOLOHH_20385: kch - voltage-gated potassium channel protein, at 4,011,534 to 4,012,787 kch Position (kb) 4010 4011 4012Strain fitness (log2 ratio) -2 -1 0 1 2at 4010.308 kb on - strand, within tonBat 4010.510 kb on - strand, within tonBat 4010.621 kb on - strand, within tonBat 4010.631 kb on - strand, within tonBat 4010.631 kb on - strand, within tonBat 4010.725 kb on - strandat 4010.725 kb on - strandat 4010.737 kb on - strandat 4010.950 kb on + strandat 4010.950 kb on + strandat 4010.951 kb on - strandat 4010.954 kb on + strandat 4011.082 kb on + strand, within yciIat 4011.356 kb on + strandat 4011.369 kb on + strandat 4011.423 kb on + strandat 4011.501 kb on + strandat 4011.775 kb on + strand, within kchat 4012.012 kb on - strand, within kchat 4012.181 kb on + strand, within kch

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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4,010,308 - tonB NOLOHH_20375 0.43 -0.4
4,010,510 - tonB NOLOHH_20375 0.71 +0.9
4,010,621 - tonB NOLOHH_20375 0.87 -2.2
4,010,631 - tonB NOLOHH_20375 0.88 -0.0
4,010,631 - tonB NOLOHH_20375 0.88 +0.5
4,010,725 - +0.2
4,010,725 - -0.1
4,010,737 - +0.5
4,010,950 + +1.9
4,010,950 + +1.0
4,010,951 - -0.6
4,010,954 + +0.2
4,011,082 + yciI NOLOHH_20380 0.48 -0.6
4,011,356 + +0.7
4,011,369 + +0.3
4,011,423 + +1.2
4,011,501 + +1.3
4,011,775 + kch NOLOHH_20385 0.19 -0.1
4,012,012 - kch NOLOHH_20385 0.38 -0.8
4,012,181 + kch NOLOHH_20385 0.52 +0.3

Or see this region's nucleotide sequence