Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20200

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdeR and yciZ are separated by 146 nucleotidesyciZ and yciT are separated by 89 nucleotidesyciT and osmB are separated by 268 nucleotidesosmB and yciH are separated by 125 nucleotidesyciH and pyrF overlap by 1 nucleotides NOLOHH_20190: pdeR - cyclic di-GMP phosphodiesterase, at 3,977,100 to 3,979,085 pdeR NOLOHH_20195: yciZ - UPF0509 protein YciZ, at 3,979,232 to 3,979,405 yciZ NOLOHH_20200: yciT - DNA-binding transcriptional regulator YciT, at 3,979,495 to 3,980,244 yciT NOLOHH_20205: osmB - osmotically-inducible lipoprotein OsmB, at 3,980,513 to 3,980,731 osmB NOLOHH_20210: yciH - stress response translation initiation inhibitor YciH, at 3,980,857 to 3,981,183 yciH NOLOHH_20215: pyrF - orotidine-5'-phosphate decarboxylase, at 3,981,183 to 3,981,980 pyrF Position (kb) 3979 3980 3981Strain fitness (log2 ratio) -2 -1 0 1 2at 3979.267 kb on + strand, within yciZat 3979.332 kb on + strand, within yciZat 3979.336 kb on + strand, within yciZat 3979.403 kb on + strandat 3979.702 kb on + strand, within yciTat 3979.702 kb on + strand, within yciTat 3979.705 kb on - strand, within yciTat 3979.929 kb on + strand, within yciTat 3980.463 kb on + strandat 3980.478 kb on + strandat 3980.677 kb on + strand, within osmBat 3980.677 kb on + strand, within osmBat 3980.764 kb on + strandat 3980.806 kb on + strandat 3981.110 kb on - strand, within yciHat 3981.110 kb on - strand, within yciH

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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3,979,267 + yciZ NOLOHH_20195 0.20 -0.0
3,979,332 + yciZ NOLOHH_20195 0.57 -0.1
3,979,336 + yciZ NOLOHH_20195 0.60 +0.8
3,979,403 + -0.9
3,979,702 + yciT NOLOHH_20200 0.28 -1.2
3,979,702 + yciT NOLOHH_20200 0.28 +1.1
3,979,705 - yciT NOLOHH_20200 0.28 +0.1
3,979,929 + yciT NOLOHH_20200 0.58 -0.7
3,980,463 + -1.6
3,980,478 + +0.9
3,980,677 + osmB NOLOHH_20205 0.75 +1.8
3,980,677 + osmB NOLOHH_20205 0.75 +0.0
3,980,764 + +0.2
3,980,806 + +0.5
3,981,110 - yciH NOLOHH_20210 0.77 -2.2
3,981,110 - yciH NOLOHH_20210 0.77 -0.9

Or see this region's nucleotide sequence