Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mddA and ydcZ overlap by 4 nucleotides ydcZ and ydcY are separated by 0 nucleotides ydcY and ortT are separated by 85 nucleotides ortT and wbbJ are separated by 691 nucleotides
NOLOHH_19205: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 3,785,876 to 3,786,394
mddA
NOLOHH_19210: ydcZ - Inner membrane protein YdcZ, at 3,786,391 to 3,786,840
ydcZ
NOLOHH_19215: ydcY - Uncharacterized protein YdcY, at 3,786,841 to 3,787,074
ydcY
NOLOHH_19220: ortT - orphan toxin OrtT, at 3,787,160 to 3,787,333
ortT
NOLOHH_19225: wbbJ - acetyltransferase, at 3,788,025 to 3,788,834
wbbJ
Position (kb)
3786
3787
3788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3785.945 kb on + strand, within mddA at 3785.947 kb on + strand, within mddA at 3786.839 kb on - strand at 3786.889 kb on - strand, within ydcY at 3787.075 kb on - strand at 3787.124 kb on - strand at 3787.493 kb on + strand at 3787.493 kb on + strand at 3787.495 kb on + strand at 3787.501 kb on + strand at 3787.513 kb on + strand at 3787.553 kb on + strand at 3787.559 kb on + strand at 3787.593 kb on + strand at 3787.648 kb on + strand at 3787.846 kb on - strand at 3788.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 3,785,945 + mddA NOLOHH_19205 0.13 +0.2 3,785,947 + mddA NOLOHH_19205 0.14 -1.8 3,786,839 - +0.8 3,786,889 - ydcY NOLOHH_19215 0.21 -1.5 3,787,075 - -0.2 3,787,124 - -1.2 3,787,493 + -1.7 3,787,493 + +2.0 3,787,495 + +0.3 3,787,501 + +1.1 3,787,513 + +0.5 3,787,553 + +1.0 3,787,559 + +0.2 3,787,593 + +0.1 3,787,648 + -1.5 3,787,846 - +0.7 3,788,027 - -2.5
Or see this region's nucleotide sequence