Strain Fitness in Escherichia coli ECOR27 around NOLOHH_19215

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmddA and ydcZ overlap by 4 nucleotidesydcZ and ydcY are separated by 0 nucleotidesydcY and ortT are separated by 85 nucleotidesortT and wbbJ are separated by 691 nucleotides NOLOHH_19205: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 3,785,876 to 3,786,394 mddA NOLOHH_19210: ydcZ - Inner membrane protein YdcZ, at 3,786,391 to 3,786,840 ydcZ NOLOHH_19215: ydcY - Uncharacterized protein YdcY, at 3,786,841 to 3,787,074 ydcY NOLOHH_19220: ortT - orphan toxin OrtT, at 3,787,160 to 3,787,333 ortT NOLOHH_19225: wbbJ - acetyltransferase, at 3,788,025 to 3,788,834 wbbJ Position (kb) 3786 3787 3788Strain fitness (log2 ratio) -2 -1 0 1 2at 3785.945 kb on + strand, within mddAat 3785.947 kb on + strand, within mddAat 3786.839 kb on - strandat 3786.889 kb on - strand, within ydcYat 3787.075 kb on - strandat 3787.124 kb on - strandat 3787.493 kb on + strandat 3787.493 kb on + strandat 3787.495 kb on + strandat 3787.501 kb on + strandat 3787.513 kb on + strandat 3787.553 kb on + strandat 3787.559 kb on + strandat 3787.593 kb on + strandat 3787.648 kb on + strandat 3787.846 kb on - strandat 3788.027 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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3,785,945 + mddA NOLOHH_19205 0.13 +0.2
3,785,947 + mddA NOLOHH_19205 0.14 -1.8
3,786,839 - +0.8
3,786,889 - ydcY NOLOHH_19215 0.21 -1.5
3,787,075 - -0.2
3,787,124 - -1.2
3,787,493 + -1.7
3,787,493 + +2.0
3,787,495 + +0.3
3,787,501 + +1.1
3,787,513 + +0.5
3,787,553 + +1.0
3,787,559 + +0.2
3,787,593 + +0.1
3,787,648 + -1.5
3,787,846 - +0.7
3,788,027 - -2.5

Or see this region's nucleotide sequence