Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NOLOHH_18040 and nth are separated by 127 nucleotides nth and rsxE overlap by 1 nucleotides rsxE and rsxG are separated by 3 nucleotides rsxG and rsxD are separated by 3 nucleotides
NOLOHH_18040: NOLOHH_18040 - hypothetical protein, at 3,540,730 to 3,540,930
_18040
NOLOHH_18045: nth - endonuclease III, at 3,541,058 to 3,541,693
nth
NOLOHH_18050: rsxE - electron transport complex subunit RsxE, at 3,541,693 to 3,542,388
rsxE
NOLOHH_18055: rsxG - electron transport complex subunit RsxG, at 3,542,392 to 3,543,012
rsxG
NOLOHH_18060: rsxD - electron transport complex subunit RsxD, at 3,543,016 to 3,544,074
rsxD
Position (kb) 3541
3542
3543 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3541.778 kb on + strand, within rsxE at 3541.779 kb on - strand, within rsxE at 3541.895 kb on - strand, within rsxE at 3542.444 kb on + strand at 3542.848 kb on - strand, within rsxG at 3543.053 kb on + strand at 3543.053 kb on + strand at 3543.146 kb on - strand, within rsxD at 3543.149 kb on - strand, within rsxD at 3543.346 kb on - strand, within rsxD
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 3,541,778 + rsxE NOLOHH_18050 0.12 +0.3 3,541,779 - rsxE NOLOHH_18050 0.12 -0.9 3,541,895 - rsxE NOLOHH_18050 0.29 -1.7 3,542,444 + -0.9 3,542,848 - rsxG NOLOHH_18055 0.73 -0.1 3,543,053 + -0.3 3,543,053 + +0.1 3,543,146 - rsxD NOLOHH_18060 0.12 -2.9 3,543,149 - rsxD NOLOHH_18060 0.13 +2.0 3,543,346 - rsxD NOLOHH_18060 0.31 -0.8
Or see this region's nucleotide sequence