Strain Fitness in Escherichia coli ECOR27 around NOLOHH_16870

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurT and yebG are separated by 133 nucleotidesyebG and yebF are separated by 66 nucleotidesyebF and NOLOHH_16875 overlap by 87 nucleotidesNOLOHH_16875 and NOLOHH_16880 are separated by 36 nucleotidesNOLOHH_16880 and yebE are separated by 59 nucleotides NOLOHH_16860: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 3,316,629 to 3,317,807 purT NOLOHH_16865: yebG - DNA damage-inducible protein YebG, at 3,317,941 to 3,318,231 yebG NOLOHH_16870: yebF - Protein YebF, at 3,318,298 to 3,318,654 yebF NOLOHH_16875: NOLOHH_16875 - hypothetical protein, at 3,318,568 to 3,318,774 _16875 NOLOHH_16880: NOLOHH_16880 - hypothetical protein, at 3,318,811 to 3,318,921 _16880 NOLOHH_16885: yebE - Inner membrane protein YebE, at 3,318,981 to 3,319,640 yebE Position (kb) 3318 3319Strain fitness (log2 ratio) -2 -1 0 1 2at 3317.553 kb on - strand, within purTat 3317.574 kb on - strand, within purTat 3317.576 kb on - strand, within purTat 3317.576 kb on - strand, within purTat 3317.576 kb on - strand, within purTat 3317.799 kb on + strandat 3317.825 kb on + strandat 3317.923 kb on + strandat 3317.955 kb on + strandat 3317.955 kb on + strandat 3317.959 kb on + strandat 3317.959 kb on + strandat 3317.959 kb on + strandat 3317.963 kb on + strandat 3318.022 kb on + strand, within yebGat 3318.257 kb on + strandat 3318.437 kb on + strand, within yebFat 3318.552 kb on + strand, within yebFat 3318.582 kb on + strandat 3318.582 kb on + strandat 3318.850 kb on - strand, within NOLOHH_16880at 3318.893 kb on - strand, within NOLOHH_16880at 3318.917 kb on + strandat 3318.947 kb on + strandat 3318.956 kb on + strandat 3318.957 kb on - strandat 3318.980 kb on + strandat 3319.034 kb on + strandat 3319.147 kb on - strand, within yebEat 3319.205 kb on + strand, within yebEat 3319.265 kb on + strand, within yebEat 3319.283 kb on + strand, within yebEat 3319.405 kb on + strand, within yebEat 3319.542 kb on + strand, within yebE

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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3,317,553 - purT NOLOHH_16860 0.78 -0.2
3,317,574 - purT NOLOHH_16860 0.80 +0.8
3,317,576 - purT NOLOHH_16860 0.80 +1.7
3,317,576 - purT NOLOHH_16860 0.80 +0.1
3,317,576 - purT NOLOHH_16860 0.80 -1.8
3,317,799 + +0.0
3,317,825 + +1.3
3,317,923 + -2.6
3,317,955 + +0.1
3,317,955 + -0.8
3,317,959 + +2.2
3,317,959 + -1.2
3,317,959 + +1.0
3,317,963 + +1.3
3,318,022 + yebG NOLOHH_16865 0.28 +0.9
3,318,257 + +0.8
3,318,437 + yebF NOLOHH_16870 0.39 +0.1
3,318,552 + yebF NOLOHH_16870 0.71 -1.5
3,318,582 + -0.3
3,318,582 + -0.1
3,318,850 - NOLOHH_16880 0.35 -1.0
3,318,893 - NOLOHH_16880 0.74 +0.6
3,318,917 + +1.6
3,318,947 + -0.4
3,318,956 + -0.9
3,318,957 - +0.3
3,318,980 + -0.7
3,319,034 + +0.9
3,319,147 - yebE NOLOHH_16885 0.25 +1.7
3,319,205 + yebE NOLOHH_16885 0.34 +1.4
3,319,265 + yebE NOLOHH_16885 0.43 -0.5
3,319,283 + yebE NOLOHH_16885 0.46 -0.2
3,319,405 + yebE NOLOHH_16885 0.64 -0.8
3,319,542 + yebE NOLOHH_16885 0.85 +0.4

Or see this region's nucleotide sequence