Strain Fitness in Escherichia coli ECOR27 around NOLOHH_16355

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliF and fliE are separated by 214 nucleotidesfliE and yedK are separated by 392 nucleotidesyedK and yedF are separated by 106 nucleotidesyedF and yedE overlap by 4 nucleotides NOLOHH_16345: fliF - flagellar M-ring protein FliF, at 3,218,426 to 3,220,084 fliF NOLOHH_16350: fliE - flagellar hook-basal body complex protein FliE, at 3,220,299 to 3,220,613 fliE NOLOHH_16355: yedK - Abasic site processing protein YedK, at 3,221,006 to 3,221,674 yedK NOLOHH_16360: yedF - sulfurtransferase-like selenium metabolism protein YedF, at 3,221,781 to 3,222,014 yedF NOLOHH_16365: yedE - selenium metabolism membrane protein YedE/FdhT, at 3,222,011 to 3,223,216 yedE Position (kb) 3221 3222Strain fitness (log2 ratio) -3 -2 -1 0 1at 3220.180 kb on - strandat 3220.231 kb on + strandat 3220.231 kb on + strandat 3220.231 kb on + strandat 3220.395 kb on - strand, within fliEat 3220.631 kb on + strandat 3220.780 kb on - strandat 3220.893 kb on + strandat 3220.910 kb on + strandat 3220.957 kb on + strandat 3221.021 kb on + strandat 3221.047 kb on + strandat 3221.201 kb on - strand, within yedKat 3221.329 kb on - strand, within yedKat 3221.419 kb on + strand, within yedKat 3221.568 kb on + strand, within yedKat 3221.706 kb on - strandat 3221.728 kb on + strandat 3221.783 kb on - strandat 3221.838 kb on - strand, within yedFat 3221.838 kb on - strand, within yedFat 3222.106 kb on - strandat 3222.106 kb on - strandat 3222.106 kb on - strandat 3222.106 kb on - strandat 3222.106 kb on - strandat 3222.201 kb on + strand, within yedEat 3222.330 kb on - strand, within yedEat 3222.342 kb on - strand, within yedEat 3222.390 kb on - strand, within yedEat 3222.390 kb on - strand, within yedEat 3222.394 kb on - strand, within yedEat 3222.472 kb on + strand, within yedEat 3222.491 kb on - strand, within yedEat 3222.491 kb on - strand, within yedE

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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3,220,180 - +0.8
3,220,231 + +1.5
3,220,231 + +0.2
3,220,231 + +0.3
3,220,395 - fliE NOLOHH_16350 0.30 -1.1
3,220,631 + +0.1
3,220,780 - -0.5
3,220,893 + +0.2
3,220,910 + -0.2
3,220,957 + +1.6
3,221,021 + -0.3
3,221,047 + +0.3
3,221,201 - yedK NOLOHH_16355 0.29 -0.2
3,221,329 - yedK NOLOHH_16355 0.48 -1.3
3,221,419 + yedK NOLOHH_16355 0.62 -1.3
3,221,568 + yedK NOLOHH_16355 0.84 +1.3
3,221,706 - -0.1
3,221,728 + -0.9
3,221,783 - +0.4
3,221,838 - yedF NOLOHH_16360 0.24 -0.5
3,221,838 - yedF NOLOHH_16360 0.24 -0.2
3,222,106 - +0.7
3,222,106 - +0.1
3,222,106 - -3.5
3,222,106 - -1.0
3,222,106 - -0.2
3,222,201 + yedE NOLOHH_16365 0.16 +0.8
3,222,330 - yedE NOLOHH_16365 0.26 +0.1
3,222,342 - yedE NOLOHH_16365 0.27 +0.2
3,222,390 - yedE NOLOHH_16365 0.31 -1.5
3,222,390 - yedE NOLOHH_16365 0.31 +0.9
3,222,394 - yedE NOLOHH_16365 0.32 -0.2
3,222,472 + yedE NOLOHH_16365 0.38 +1.1
3,222,491 - yedE NOLOHH_16365 0.40 +1.5
3,222,491 - yedE NOLOHH_16365 0.40 -2.0

Or see this region's nucleotide sequence