Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliF and fliE are separated by 214 nucleotides fliE and yedK are separated by 392 nucleotides yedK and yedF are separated by 106 nucleotides yedF and yedE overlap by 4 nucleotides
NOLOHH_16345: fliF - flagellar M-ring protein FliF, at 3,218,426 to 3,220,084
fliF
NOLOHH_16350: fliE - flagellar hook-basal body complex protein FliE, at 3,220,299 to 3,220,613
fliE
NOLOHH_16355: yedK - Abasic site processing protein YedK, at 3,221,006 to 3,221,674
yedK
NOLOHH_16360: yedF - sulfurtransferase-like selenium metabolism protein YedF, at 3,221,781 to 3,222,014
yedF
NOLOHH_16365: yedE - selenium metabolism membrane protein YedE/FdhT, at 3,222,011 to 3,223,216
yedE
Position (kb)
3221
3222 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3220.180 kb on - strand at 3220.231 kb on + strand at 3220.231 kb on + strand at 3220.231 kb on + strand at 3220.395 kb on - strand, within fliE at 3220.631 kb on + strand at 3220.780 kb on - strand at 3220.893 kb on + strand at 3220.910 kb on + strand at 3220.957 kb on + strand at 3221.021 kb on + strand at 3221.047 kb on + strand at 3221.201 kb on - strand, within yedK at 3221.329 kb on - strand, within yedK at 3221.419 kb on + strand, within yedK at 3221.568 kb on + strand, within yedK at 3221.706 kb on - strand at 3221.728 kb on + strand at 3221.783 kb on - strand at 3221.838 kb on - strand, within yedF at 3221.838 kb on - strand, within yedF at 3222.106 kb on - strand at 3222.106 kb on - strand at 3222.106 kb on - strand at 3222.106 kb on - strand at 3222.106 kb on - strand at 3222.201 kb on + strand, within yedE at 3222.330 kb on - strand, within yedE at 3222.342 kb on - strand, within yedE at 3222.390 kb on - strand, within yedE at 3222.390 kb on - strand, within yedE at 3222.394 kb on - strand, within yedE at 3222.472 kb on + strand, within yedE at 3222.491 kb on - strand, within yedE at 3222.491 kb on - strand, within yedE
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 3,220,180 - +0.8 3,220,231 + +1.5 3,220,231 + +0.2 3,220,231 + +0.3 3,220,395 - fliE NOLOHH_16350 0.30 -1.1 3,220,631 + +0.1 3,220,780 - -0.5 3,220,893 + +0.2 3,220,910 + -0.2 3,220,957 + +1.6 3,221,021 + -0.3 3,221,047 + +0.3 3,221,201 - yedK NOLOHH_16355 0.29 -0.2 3,221,329 - yedK NOLOHH_16355 0.48 -1.3 3,221,419 + yedK NOLOHH_16355 0.62 -1.3 3,221,568 + yedK NOLOHH_16355 0.84 +1.3 3,221,706 - -0.1 3,221,728 + -0.9 3,221,783 - +0.4 3,221,838 - yedF NOLOHH_16360 0.24 -0.5 3,221,838 - yedF NOLOHH_16360 0.24 -0.2 3,222,106 - +0.7 3,222,106 - +0.1 3,222,106 - -3.5 3,222,106 - -1.0 3,222,106 - -0.2 3,222,201 + yedE NOLOHH_16365 0.16 +0.8 3,222,330 - yedE NOLOHH_16365 0.26 +0.1 3,222,342 - yedE NOLOHH_16365 0.27 +0.2 3,222,390 - yedE NOLOHH_16365 0.31 -1.5 3,222,390 - yedE NOLOHH_16365 0.31 +0.9 3,222,394 - yedE NOLOHH_16365 0.32 -0.2 3,222,472 + yedE NOLOHH_16365 0.38 +1.1 3,222,491 - yedE NOLOHH_16365 0.40 +1.5 3,222,491 - yedE NOLOHH_16365 0.40 -2.0
Or see this region's nucleotide sequence