Strain Fitness in Escherichia coli ECOR27 around NOLOHH_16015

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpduB and pduA overlap by 4 nucleotidespduA and pduF are separated by 511 nucleotidespduF and pocR are separated by 202 nucleotides NOLOHH_16005: pduB - propanediol utilization microcompartment protein PduB, at 3,147,698 to 3,148,510 pduB NOLOHH_16010: pduA - propanediol utilization microcompartment protein PduA, at 3,148,507 to 3,148,791 pduA NOLOHH_16015: pduF - propanediol diffusion facilitator PduF, at 3,149,303 to 3,150,112 pduF NOLOHH_16025: pocR - regulatory protein PocR, at 3,150,315 to 3,151,229 pocR Position (kb) 3149 3150 3151Strain fitness (log2 ratio) -2 -1 0 1at 3148.340 kb on + strand, within pduBat 3148.341 kb on - strand, within pduBat 3148.548 kb on + strand, within pduAat 3148.549 kb on - strand, within pduAat 3148.751 kb on - strand, within pduAat 3148.751 kb on - strand, within pduAat 3148.829 kb on - strandat 3149.261 kb on + strandat 3149.261 kb on + strandat 3149.730 kb on + strand, within pduFat 3149.991 kb on - strand, within pduFat 3150.284 kb on + strandat 3150.548 kb on + strand, within pocRat 3150.585 kb on - strand, within pocRat 3150.716 kb on + strand, within pocRat 3151.072 kb on + strand, within pocR

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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3,148,340 + pduB NOLOHH_16005 0.79 -0.2
3,148,341 - pduB NOLOHH_16005 0.79 +0.2
3,148,548 + pduA NOLOHH_16010 0.14 -0.6
3,148,549 - pduA NOLOHH_16010 0.15 -0.8
3,148,751 - pduA NOLOHH_16010 0.86 -0.4
3,148,751 - pduA NOLOHH_16010 0.86 -1.2
3,148,829 - +0.5
3,149,261 + +0.3
3,149,261 + +0.4
3,149,730 + pduF NOLOHH_16015 0.53 -0.9
3,149,991 - pduF NOLOHH_16015 0.85 -2.3
3,150,284 + +1.0
3,150,548 + pocR NOLOHH_16025 0.25 +0.7
3,150,585 - pocR NOLOHH_16025 0.30 -0.6
3,150,716 + pocR NOLOHH_16025 0.44 +0.5
3,151,072 + pocR NOLOHH_16025 0.83 +0.4

Or see this region's nucleotide sequence