Strain Fitness in Escherichia coli ECOR27 around NOLOHH_11425

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiH and ubiI are separated by 22 nucleotidesubiI and yqfI are separated by 73 nucleotidesyqfI and gcvT are separated by 289 nucleotides NOLOHH_11420: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 2,207,028 to 2,208,206 ubiH NOLOHH_11425: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 2,208,229 to 2,209,431 ubiI NOLOHH_11430: yqfI - Protein YqfI, at 2,209,505 to 2,209,588 yqfI NOLOHH_11435: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 2,209,878 to 2,210,972 gcvT Position (kb) 2208 2209 2210Strain fitness (log2 ratio) -2 -1 0 1at 2208.148 kb on + strandat 2208.148 kb on + strandat 2208.223 kb on + strandat 2208.223 kb on + strandat 2208.245 kb on + strandat 2208.245 kb on + strandat 2208.245 kb on + strandat 2208.246 kb on - strandat 2208.460 kb on + strand, within ubiIat 2208.754 kb on - strand, within ubiIat 2208.974 kb on + strand, within ubiIat 2209.004 kb on + strand, within ubiIat 2209.005 kb on - strand, within ubiIat 2209.276 kb on - strand, within ubiIat 2209.384 kb on - strandat 2209.384 kb on - strandat 2209.403 kb on + strandat 2209.403 kb on + strandat 2209.452 kb on - strandat 2209.707 kb on + strandat 2209.900 kb on - strandat 2209.965 kb on + strandat 2209.966 kb on - strandat 2210.030 kb on + strand, within gcvTat 2210.030 kb on + strand, within gcvTat 2210.030 kb on + strand, within gcvTat 2210.030 kb on + strand, within gcvTat 2210.031 kb on - strand, within gcvTat 2210.031 kb on - strand, within gcvTat 2210.031 kb on - strand, within gcvTat 2210.031 kb on - strand, within gcvTat 2210.031 kb on - strand, within gcvTat 2210.031 kb on - strand, within gcvTat 2210.031 kb on - strand, within gcvTat 2210.079 kb on + strand, within gcvTat 2210.130 kb on + strand, within gcvTat 2210.164 kb on + strand, within gcvTat 2210.164 kb on + strand, within gcvTat 2210.164 kb on + strand, within gcvTat 2210.164 kb on + strand, within gcvTat 2210.165 kb on - strand, within gcvTat 2210.165 kb on - strand, within gcvTat 2210.165 kb on - strand, within gcvTat 2210.165 kb on - strand, within gcvTat 2210.182 kb on - strand, within gcvTat 2210.182 kb on - strand, within gcvTat 2210.189 kb on + strand, within gcvTat 2210.190 kb on - strand, within gcvTat 2210.190 kb on - strand, within gcvTat 2210.217 kb on - strand, within gcvTat 2210.285 kb on + strand, within gcvTat 2210.358 kb on - strand, within gcvT

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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2,208,148 + -0.5
2,208,148 + -0.3
2,208,223 + -1.5
2,208,223 + -2.3
2,208,245 + +0.1
2,208,245 + -1.2
2,208,245 + -1.8
2,208,246 - -0.2
2,208,460 + ubiI NOLOHH_11425 0.19 -1.1
2,208,754 - ubiI NOLOHH_11425 0.44 +1.7
2,208,974 + ubiI NOLOHH_11425 0.62 -0.6
2,209,004 + ubiI NOLOHH_11425 0.64 -0.7
2,209,005 - ubiI NOLOHH_11425 0.65 -0.6
2,209,276 - ubiI NOLOHH_11425 0.87 -1.7
2,209,384 - +0.8
2,209,384 - -0.7
2,209,403 + +0.7
2,209,403 + -0.6
2,209,452 - -1.0
2,209,707 + +0.8
2,209,900 - +0.6
2,209,965 + -0.1
2,209,966 - -0.4
2,210,030 + gcvT NOLOHH_11435 0.14 +0.1
2,210,030 + gcvT NOLOHH_11435 0.14 -0.2
2,210,030 + gcvT NOLOHH_11435 0.14 -0.0
2,210,030 + gcvT NOLOHH_11435 0.14 -1.9
2,210,031 - gcvT NOLOHH_11435 0.14 -0.5
2,210,031 - gcvT NOLOHH_11435 0.14 +1.1
2,210,031 - gcvT NOLOHH_11435 0.14 +0.2
2,210,031 - gcvT NOLOHH_11435 0.14 -0.8
2,210,031 - gcvT NOLOHH_11435 0.14 +1.2
2,210,031 - gcvT NOLOHH_11435 0.14 +0.1
2,210,031 - gcvT NOLOHH_11435 0.14 -0.2
2,210,079 + gcvT NOLOHH_11435 0.18 +0.7
2,210,130 + gcvT NOLOHH_11435 0.23 +0.6
2,210,164 + gcvT NOLOHH_11435 0.26 +0.0
2,210,164 + gcvT NOLOHH_11435 0.26 -1.1
2,210,164 + gcvT NOLOHH_11435 0.26 +0.3
2,210,164 + gcvT NOLOHH_11435 0.26 -0.2
2,210,165 - gcvT NOLOHH_11435 0.26 +0.8
2,210,165 - gcvT NOLOHH_11435 0.26 -0.0
2,210,165 - gcvT NOLOHH_11435 0.26 -1.3
2,210,165 - gcvT NOLOHH_11435 0.26 -0.3
2,210,182 - gcvT NOLOHH_11435 0.28 +0.4
2,210,182 - gcvT NOLOHH_11435 0.28 +0.0
2,210,189 + gcvT NOLOHH_11435 0.28 +1.0
2,210,190 - gcvT NOLOHH_11435 0.28 -0.1
2,210,190 - gcvT NOLOHH_11435 0.28 -0.3
2,210,217 - gcvT NOLOHH_11435 0.31 +1.7
2,210,285 + gcvT NOLOHH_11435 0.37 +0.1
2,210,358 - gcvT NOLOHH_11435 0.44 -0.4

Or see this region's nucleotide sequence