Strain Fitness in Escherichia coli ECOR27 around NOLOHH_07575

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaL and rfaC overlap by 4 nucleotidesrfaC and rfaF are separated by 3 nucleotidesrfaF and rfaD are separated by 9 nucleotides NOLOHH_07565: rfaL - O-antigen ligase RfaL, at 1,438,728 to 1,439,936 rfaL NOLOHH_07570: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 1,439,933 to 1,440,925 rfaC NOLOHH_07575: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 1,440,929 to 1,441,975 rfaF NOLOHH_07580: rfaD - ADP-glyceromanno-heptose 6-epimerase, at 1,441,985 to 1,442,917 rfaD Position (kb) 1440 1441 1442Strain fitness (log2 ratio) -1 0 1 2at 1440.945 kb on - strandat 1441.272 kb on - strand, within rfaFat 1441.463 kb on - strand, within rfaFat 1441.469 kb on - strand, within rfaFat 1441.471 kb on - strand, within rfaFat 1441.568 kb on + strand, within rfaFat 1441.569 kb on - strand, within rfaFat 1441.571 kb on - strand, within rfaFat 1441.617 kb on - strand, within rfaFat 1441.617 kb on - strand, within rfaFat 1441.987 kb on - strandat 1441.987 kb on - strandat 1442.934 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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1,440,945 - -0.9
1,441,272 - rfaF NOLOHH_07575 0.33 +0.5
1,441,463 - rfaF NOLOHH_07575 0.51 -0.9
1,441,469 - rfaF NOLOHH_07575 0.52 +0.1
1,441,471 - rfaF NOLOHH_07575 0.52 -0.1
1,441,568 + rfaF NOLOHH_07575 0.61 +0.5
1,441,569 - rfaF NOLOHH_07575 0.61 -1.7
1,441,571 - rfaF NOLOHH_07575 0.61 -0.8
1,441,617 - rfaF NOLOHH_07575 0.66 -0.5
1,441,617 - rfaF NOLOHH_07575 0.66 +1.9
1,441,987 - +0.4
1,441,987 - -1.2
1,442,934 - -0.4

Or see this region's nucleotide sequence