Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaB and rfaL are separated by 35 nucleotides rfaL and rfaC overlap by 4 nucleotides rfaC and rfaF are separated by 3 nucleotides
NOLOHH_07560: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 1,437,550 to 1,438,692
rfaB
NOLOHH_07565: rfaL - O-antigen ligase RfaL, at 1,438,728 to 1,439,936
rfaL
NOLOHH_07570: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 1,439,933 to 1,440,925
rfaC
NOLOHH_07575: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 1,440,929 to 1,441,975
rfaF
Position (kb)
1438
1439
1440 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1437.870 kb on + strand, within rfaB at 1437.870 kb on + strand, within rfaB at 1437.883 kb on + strand, within rfaB at 1438.072 kb on + strand, within rfaB at 1438.114 kb on - strand, within rfaB at 1438.173 kb on - strand, within rfaB at 1438.230 kb on - strand, within rfaB at 1438.329 kb on + strand, within rfaB at 1438.366 kb on + strand, within rfaB at 1438.367 kb on - strand, within rfaB at 1438.470 kb on + strand, within rfaB at 1438.510 kb on - strand, within rfaB at 1438.533 kb on + strand, within rfaB at 1438.677 kb on + strand at 1438.846 kb on + strand at 1438.855 kb on + strand, within rfaL at 1438.906 kb on - strand, within rfaL at 1439.038 kb on + strand, within rfaL at 1439.048 kb on + strand, within rfaL at 1439.061 kb on - strand, within rfaL at 1439.098 kb on - strand, within rfaL at 1439.145 kb on - strand, within rfaL at 1439.236 kb on - strand, within rfaL at 1439.494 kb on + strand, within rfaL at 1439.495 kb on - strand, within rfaL at 1439.498 kb on - strand, within rfaL at 1439.608 kb on - strand, within rfaL at 1439.617 kb on - strand, within rfaL at 1439.624 kb on + strand, within rfaL at 1439.637 kb on - strand, within rfaL at 1439.823 kb on - strand at 1439.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 1,437,870 + rfaB NOLOHH_07560 0.28 -1.4 1,437,870 + rfaB NOLOHH_07560 0.28 -0.2 1,437,883 + rfaB NOLOHH_07560 0.29 -2.2 1,438,072 + rfaB NOLOHH_07560 0.46 -0.8 1,438,114 - rfaB NOLOHH_07560 0.49 +0.4 1,438,173 - rfaB NOLOHH_07560 0.55 -1.1 1,438,230 - rfaB NOLOHH_07560 0.59 -0.5 1,438,329 + rfaB NOLOHH_07560 0.68 -3.0 1,438,366 + rfaB NOLOHH_07560 0.71 +0.2 1,438,367 - rfaB NOLOHH_07560 0.71 -0.1 1,438,470 + rfaB NOLOHH_07560 0.80 +0.2 1,438,510 - rfaB NOLOHH_07560 0.84 -3.2 1,438,533 + rfaB NOLOHH_07560 0.86 -0.9 1,438,677 + +0.5 1,438,846 + -1.2 1,438,855 + rfaL NOLOHH_07565 0.11 -2.3 1,438,906 - rfaL NOLOHH_07565 0.15 -2.0 1,439,038 + rfaL NOLOHH_07565 0.26 -1.5 1,439,048 + rfaL NOLOHH_07565 0.26 -0.9 1,439,061 - rfaL NOLOHH_07565 0.28 -1.0 1,439,098 - rfaL NOLOHH_07565 0.31 -1.6 1,439,145 - rfaL NOLOHH_07565 0.34 -0.8 1,439,236 - rfaL NOLOHH_07565 0.42 -0.3 1,439,494 + rfaL NOLOHH_07565 0.63 -2.5 1,439,495 - rfaL NOLOHH_07565 0.63 -0.1 1,439,498 - rfaL NOLOHH_07565 0.64 -1.8 1,439,608 - rfaL NOLOHH_07565 0.73 +1.1 1,439,617 - rfaL NOLOHH_07565 0.74 -0.7 1,439,624 + rfaL NOLOHH_07565 0.74 -0.2 1,439,637 - rfaL NOLOHH_07565 0.75 -1.6 1,439,823 - -0.7 1,439,863 - +0.0
Or see this region's nucleotide sequence