Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides rfaJ and rfaB are separated by 8 nucleotides
NOLOHH_07545: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,434,763 to 1,435,770
waaO
NOLOHH_07550: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,435,796 to 1,436,503
rfaY
NOLOHH_07555: rfaJ - glucosyltransferase, at 1,436,528 to 1,437,541
rfaJ
NOLOHH_07560: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 1,437,550 to 1,438,692
rfaB
Position (kb)
1436
1437
1438 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1435.552 kb on + strand, within waaO at 1435.747 kb on + strand at 1435.802 kb on + strand at 1435.881 kb on + strand, within rfaY at 1435.972 kb on + strand, within rfaY at 1436.152 kb on + strand, within rfaY at 1436.166 kb on - strand, within rfaY at 1436.392 kb on + strand, within rfaY at 1436.430 kb on - strand, within rfaY at 1436.525 kb on - strand at 1436.525 kb on - strand at 1436.749 kb on - strand, within rfaJ at 1437.136 kb on - strand, within rfaJ at 1437.321 kb on + strand, within rfaJ at 1437.328 kb on + strand, within rfaJ at 1437.523 kb on + strand at 1437.529 kb on + strand at 1437.717 kb on + strand, within rfaB at 1437.726 kb on + strand, within rfaB at 1437.870 kb on + strand, within rfaB at 1437.870 kb on + strand, within rfaB at 1437.883 kb on + strand, within rfaB at 1438.072 kb on + strand, within rfaB at 1438.114 kb on - strand, within rfaB at 1438.173 kb on - strand, within rfaB at 1438.230 kb on - strand, within rfaB at 1438.329 kb on + strand, within rfaB at 1438.366 kb on + strand, within rfaB at 1438.367 kb on - strand, within rfaB at 1438.470 kb on + strand, within rfaB at 1438.510 kb on - strand, within rfaB at 1438.533 kb on + strand, within rfaB
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 1,435,552 + waaO NOLOHH_07545 0.78 -1.5 1,435,747 + +0.4 1,435,802 + +1.0 1,435,881 + rfaY NOLOHH_07550 0.12 -1.0 1,435,972 + rfaY NOLOHH_07550 0.25 -1.6 1,436,152 + rfaY NOLOHH_07550 0.50 -1.9 1,436,166 - rfaY NOLOHH_07550 0.52 -0.7 1,436,392 + rfaY NOLOHH_07550 0.84 -0.9 1,436,430 - rfaY NOLOHH_07550 0.90 +0.8 1,436,525 - +0.1 1,436,525 - -0.7 1,436,749 - rfaJ NOLOHH_07555 0.22 -1.6 1,437,136 - rfaJ NOLOHH_07555 0.60 -0.8 1,437,321 + rfaJ NOLOHH_07555 0.78 -2.0 1,437,328 + rfaJ NOLOHH_07555 0.79 -1.2 1,437,523 + +0.9 1,437,529 + -0.3 1,437,717 + rfaB NOLOHH_07560 0.15 -0.9 1,437,726 + rfaB NOLOHH_07560 0.15 -0.7 1,437,870 + rfaB NOLOHH_07560 0.28 -1.4 1,437,870 + rfaB NOLOHH_07560 0.28 -0.2 1,437,883 + rfaB NOLOHH_07560 0.29 -2.2 1,438,072 + rfaB NOLOHH_07560 0.46 -0.8 1,438,114 - rfaB NOLOHH_07560 0.49 +0.4 1,438,173 - rfaB NOLOHH_07560 0.55 -1.1 1,438,230 - rfaB NOLOHH_07560 0.59 -0.5 1,438,329 + rfaB NOLOHH_07560 0.68 -3.0 1,438,366 + rfaB NOLOHH_07560 0.71 +0.2 1,438,367 - rfaB NOLOHH_07560 0.71 -0.1 1,438,470 + rfaB NOLOHH_07560 0.80 +0.2 1,438,510 - rfaB NOLOHH_07560 0.84 -3.2 1,438,533 + rfaB NOLOHH_07560 0.86 -0.9
Or see this region's nucleotide sequence