Strain Fitness in Escherichia coli ECOR27 around NOLOHH_07555

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotidesrfaJ and rfaB are separated by 8 nucleotides NOLOHH_07545: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,434,763 to 1,435,770 waaO NOLOHH_07550: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,435,796 to 1,436,503 rfaY NOLOHH_07555: rfaJ - glucosyltransferase, at 1,436,528 to 1,437,541 rfaJ NOLOHH_07560: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 1,437,550 to 1,438,692 rfaB Position (kb) 1436 1437 1438Strain fitness (log2 ratio) -3 -2 -1 0 1at 1435.552 kb on + strand, within waaOat 1435.747 kb on + strandat 1435.802 kb on + strandat 1435.881 kb on + strand, within rfaYat 1435.972 kb on + strand, within rfaYat 1436.152 kb on + strand, within rfaYat 1436.166 kb on - strand, within rfaYat 1436.392 kb on + strand, within rfaYat 1436.430 kb on - strand, within rfaYat 1436.525 kb on - strandat 1436.525 kb on - strandat 1436.749 kb on - strand, within rfaJat 1437.136 kb on - strand, within rfaJat 1437.321 kb on + strand, within rfaJat 1437.328 kb on + strand, within rfaJat 1437.523 kb on + strandat 1437.529 kb on + strandat 1437.717 kb on + strand, within rfaBat 1437.726 kb on + strand, within rfaBat 1437.870 kb on + strand, within rfaBat 1437.870 kb on + strand, within rfaBat 1437.883 kb on + strand, within rfaBat 1438.072 kb on + strand, within rfaBat 1438.114 kb on - strand, within rfaBat 1438.173 kb on - strand, within rfaBat 1438.230 kb on - strand, within rfaBat 1438.329 kb on + strand, within rfaBat 1438.366 kb on + strand, within rfaBat 1438.367 kb on - strand, within rfaBat 1438.470 kb on + strand, within rfaBat 1438.510 kb on - strand, within rfaBat 1438.533 kb on + strand, within rfaB

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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1,435,552 + waaO NOLOHH_07545 0.78 -1.5
1,435,747 + +0.4
1,435,802 + +1.0
1,435,881 + rfaY NOLOHH_07550 0.12 -1.0
1,435,972 + rfaY NOLOHH_07550 0.25 -1.6
1,436,152 + rfaY NOLOHH_07550 0.50 -1.9
1,436,166 - rfaY NOLOHH_07550 0.52 -0.7
1,436,392 + rfaY NOLOHH_07550 0.84 -0.9
1,436,430 - rfaY NOLOHH_07550 0.90 +0.8
1,436,525 - +0.1
1,436,525 - -0.7
1,436,749 - rfaJ NOLOHH_07555 0.22 -1.6
1,437,136 - rfaJ NOLOHH_07555 0.60 -0.8
1,437,321 + rfaJ NOLOHH_07555 0.78 -2.0
1,437,328 + rfaJ NOLOHH_07555 0.79 -1.2
1,437,523 + +0.9
1,437,529 + -0.3
1,437,717 + rfaB NOLOHH_07560 0.15 -0.9
1,437,726 + rfaB NOLOHH_07560 0.15 -0.7
1,437,870 + rfaB NOLOHH_07560 0.28 -1.4
1,437,870 + rfaB NOLOHH_07560 0.28 -0.2
1,437,883 + rfaB NOLOHH_07560 0.29 -2.2
1,438,072 + rfaB NOLOHH_07560 0.46 -0.8
1,438,114 - rfaB NOLOHH_07560 0.49 +0.4
1,438,173 - rfaB NOLOHH_07560 0.55 -1.1
1,438,230 - rfaB NOLOHH_07560 0.59 -0.5
1,438,329 + rfaB NOLOHH_07560 0.68 -3.0
1,438,366 + rfaB NOLOHH_07560 0.71 +0.2
1,438,367 - rfaB NOLOHH_07560 0.71 -0.1
1,438,470 + rfaB NOLOHH_07560 0.80 +0.2
1,438,510 - rfaB NOLOHH_07560 0.84 -3.2
1,438,533 + rfaB NOLOHH_07560 0.86 -0.9

Or see this region's nucleotide sequence