Strain Fitness in Escherichia coli ECOR27 around NOLOHH_00545

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNOLOHH_00530 and fII are separated by 11 nucleotidesfII and NOLOHH_00540 are separated by 11 nucleotidesNOLOHH_00540 and NOLOHH_00545 are separated by 41 nucleotidesNOLOHH_00545 and NOLOHH_00550 are separated by 55 nucleotidesNOLOHH_00550 and NOLOHH_00555 are separated by 9 nucleotides NOLOHH_00530: NOLOHH_00530 - Tail completion protein Z, at 9,906 to 10,484 _00530 NOLOHH_00535: fII - Head-tail connector protein FII, at 10,496 to 10,849 fII NOLOHH_00540: NOLOHH_00540 - DNA-packaging protein FI, at 10,861 to 11,259 _00540 NOLOHH_00545: NOLOHH_00545 - Major capsid protein, at 11,301 to 12,326 _00545 NOLOHH_00550: NOLOHH_00550 - Capsid decoration protein, at 12,382 to 12,714 _00550 NOLOHH_00555: NOLOHH_00555 - Capsid assembly protease C, at 12,724 to 14,043 _00555 Position (kb) 11 12 13Strain fitness (log2 ratio) -1 0 1at 10.581 kb on - strand, within fIIat 11.605 kb on + strand, within NOLOHH_00545at 11.605 kb on + strand, within NOLOHH_00545at 11.605 kb on + strand, within NOLOHH_00545at 11.605 kb on + strand, within NOLOHH_00545

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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10,581 - fII NOLOHH_00535 0.24 -0.2
11,605 + NOLOHH_00545 0.30 -0.7
11,605 + NOLOHH_00545 0.30 -0.8
11,605 + NOLOHH_00545 0.30 -1.2
11,605 + NOLOHH_00545 0.30 +0.5

Or see this region's nucleotide sequence