Strain Fitness in Escherichia coli ECOR27 around NOLOHH_11420

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpepP and ubiH overlap by 4 nucleotidesubiH and ubiI are separated by 22 nucleotides NOLOHH_11415: pepP - Xaa-Pro aminopeptidase, at 2,205,706 to 2,207,031 pepP NOLOHH_11420: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 2,207,028 to 2,208,206 ubiH NOLOHH_11425: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 2,208,229 to 2,209,431 ubiI Position (kb) 2207 2208 2209Strain fitness (log2 ratio) -3 -2 -1 0 1at 2206.114 kb on + strand, within pepPat 2206.114 kb on + strand, within pepPat 2206.393 kb on + strand, within pepPat 2206.501 kb on + strand, within pepPat 2206.576 kb on + strand, within pepPat 2206.781 kb on + strand, within pepPat 2206.781 kb on + strand, within pepPat 2206.804 kb on + strand, within pepPat 2206.867 kb on + strand, within pepPat 2206.867 kb on + strand, within pepPat 2206.867 kb on + strand, within pepPat 2208.148 kb on + strandat 2208.148 kb on + strandat 2208.223 kb on + strandat 2208.223 kb on + strandat 2208.245 kb on + strandat 2208.245 kb on + strandat 2208.245 kb on + strandat 2208.246 kb on - strandat 2208.460 kb on + strand, within ubiIat 2208.754 kb on - strand, within ubiIat 2208.974 kb on + strand, within ubiIat 2209.004 kb on + strand, within ubiIat 2209.005 kb on - strand, within ubiI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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2,206,114 + pepP NOLOHH_11415 0.31 -1.6
2,206,114 + pepP NOLOHH_11415 0.31 -1.9
2,206,393 + pepP NOLOHH_11415 0.52 -2.4
2,206,501 + pepP NOLOHH_11415 0.60 -2.7
2,206,576 + pepP NOLOHH_11415 0.66 -1.6
2,206,781 + pepP NOLOHH_11415 0.81 -0.6
2,206,781 + pepP NOLOHH_11415 0.81 -0.0
2,206,804 + pepP NOLOHH_11415 0.83 -3.5
2,206,867 + pepP NOLOHH_11415 0.88 -0.5
2,206,867 + pepP NOLOHH_11415 0.88 -1.1
2,206,867 + pepP NOLOHH_11415 0.88 -0.7
2,208,148 + -0.8
2,208,148 + +0.3
2,208,223 + -1.6
2,208,223 + -1.3
2,208,245 + +1.7
2,208,245 + -0.3
2,208,245 + -1.0
2,208,246 - -1.8
2,208,460 + ubiI NOLOHH_11425 0.19 +0.8
2,208,754 - ubiI NOLOHH_11425 0.44 +0.1
2,208,974 + ubiI NOLOHH_11425 0.62 -1.7
2,209,004 + ubiI NOLOHH_11425 0.64 +0.8
2,209,005 - ubiI NOLOHH_11425 0.65 -0.7

Or see this region's nucleotide sequence