Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xerD and dsbC are separated by 24 nucleotides dsbC and recJ are separated by 5 nucleotides
NIAGMN_17335: xerD - site-specific tyrosine recombinase XerD, at 3,423,838 to 3,424,734
xerD
NIAGMN_17340: dsbC - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 3,424,759 to 3,425,469
dsbC
NIAGMN_17345: recJ - single-stranded-DNA-specific exonuclease RecJ, at 3,425,475 to 3,427,208
recJ
Position (kb)
3424
3425
3426 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3423.769 kb on + strand at 3423.770 kb on - strand at 3423.770 kb on - strand at 3423.770 kb on - strand at 3423.784 kb on - strand at 3423.798 kb on - strand at 3423.804 kb on + strand at 3423.804 kb on + strand at 3423.826 kb on + strand at 3423.826 kb on + strand at 3423.826 kb on + strand at 3424.248 kb on + strand, within xerD at 3424.265 kb on - strand, within xerD at 3424.638 kb on - strand, within xerD at 3424.758 kb on + strand at 3424.773 kb on + strand at 3424.773 kb on + strand at 3424.773 kb on + strand at 3424.773 kb on + strand at 3424.773 kb on + strand at 3424.774 kb on - strand at 3424.774 kb on - strand at 3424.774 kb on - strand at 3424.782 kb on + strand at 3424.783 kb on - strand at 3424.889 kb on + strand, within dsbC at 3424.914 kb on + strand, within dsbC at 3424.930 kb on + strand, within dsbC at 3424.930 kb on + strand, within dsbC at 3424.930 kb on + strand, within dsbC at 3424.930 kb on + strand, within dsbC at 3424.931 kb on - strand, within dsbC at 3424.947 kb on + strand, within dsbC at 3424.947 kb on + strand, within dsbC at 3424.947 kb on + strand, within dsbC at 3424.947 kb on + strand, within dsbC at 3424.953 kb on + strand, within dsbC at 3424.953 kb on + strand, within dsbC at 3424.953 kb on + strand, within dsbC at 3424.953 kb on + strand, within dsbC at 3424.953 kb on + strand, within dsbC at 3424.953 kb on + strand, within dsbC at 3424.953 kb on + strand, within dsbC at 3424.954 kb on - strand, within dsbC at 3424.954 kb on - strand, within dsbC at 3424.954 kb on - strand, within dsbC at 3424.954 kb on - strand, within dsbC at 3424.954 kb on - strand, within dsbC at 3424.972 kb on + strand, within dsbC at 3424.972 kb on + strand, within dsbC at 3424.972 kb on + strand, within dsbC at 3424.972 kb on + strand, within dsbC at 3424.972 kb on + strand, within dsbC at 3424.972 kb on + strand, within dsbC at 3424.972 kb on + strand, within dsbC at 3424.973 kb on - strand, within dsbC at 3424.973 kb on - strand, within dsbC at 3424.973 kb on - strand, within dsbC at 3424.973 kb on - strand, within dsbC at 3425.010 kb on + strand, within dsbC at 3425.010 kb on + strand, within dsbC at 3425.010 kb on + strand, within dsbC at 3425.010 kb on + strand, within dsbC at 3425.011 kb on - strand, within dsbC at 3425.021 kb on + strand, within dsbC at 3425.022 kb on - strand, within dsbC at 3425.062 kb on - strand, within dsbC at 3425.097 kb on + strand, within dsbC at 3425.097 kb on + strand, within dsbC at 3425.103 kb on + strand, within dsbC at 3425.104 kb on - strand, within dsbC at 3425.104 kb on - strand, within dsbC at 3425.106 kb on + strand, within dsbC at 3425.106 kb on + strand, within dsbC at 3425.106 kb on + strand, within dsbC at 3425.106 kb on + strand, within dsbC at 3425.107 kb on - strand, within dsbC at 3425.149 kb on + strand, within dsbC at 3425.149 kb on + strand, within dsbC at 3425.149 kb on + strand, within dsbC at 3425.149 kb on + strand, within dsbC at 3425.149 kb on + strand, within dsbC at 3425.149 kb on + strand, within dsbC at 3425.149 kb on + strand, within dsbC at 3425.150 kb on - strand, within dsbC at 3425.150 kb on - strand, within dsbC at 3425.150 kb on - strand, within dsbC at 3425.150 kb on - strand, within dsbC at 3425.151 kb on + strand, within dsbC at 3425.151 kb on + strand, within dsbC at 3425.177 kb on - strand, within dsbC at 3425.232 kb on + strand, within dsbC at 3425.284 kb on - strand, within dsbC at 3425.316 kb on + strand, within dsbC at 3425.329 kb on + strand, within dsbC at 3425.467 kb on + strand at 3425.468 kb on - strand at 3425.488 kb on + strand at 3425.488 kb on + strand at 3425.489 kb on - strand at 3425.489 kb on - strand at 3425.489 kb on - strand at 3425.489 kb on - strand at 3425.561 kb on - strand at 3425.562 kb on + strand at 3425.578 kb on + strand at 3425.578 kb on + strand at 3425.579 kb on - strand at 3425.615 kb on + strand at 3425.615 kb on + strand at 3425.667 kb on + strand, within recJ at 3425.667 kb on + strand, within recJ at 3425.667 kb on + strand, within recJ at 3425.668 kb on - strand, within recJ at 3425.668 kb on - strand, within recJ at 3425.696 kb on + strand, within recJ at 3425.697 kb on - strand, within recJ at 3425.697 kb on - strand, within recJ at 3425.743 kb on + strand, within recJ at 3425.743 kb on + strand, within recJ at 3425.869 kb on + strand, within recJ at 3425.869 kb on + strand, within recJ at 3425.891 kb on + strand, within recJ at 3425.891 kb on + strand, within recJ at 3425.892 kb on - strand, within recJ at 3426.006 kb on - strand, within recJ at 3426.063 kb on + strand, within recJ at 3426.063 kb on + strand, within recJ at 3426.063 kb on + strand, within recJ at 3426.064 kb on - strand, within recJ at 3426.064 kb on - strand, within recJ at 3426.134 kb on + strand, within recJ at 3426.201 kb on - strand, within recJ at 3426.201 kb on - strand, within recJ at 3426.203 kb on + strand, within recJ at 3426.322 kb on + strand, within recJ at 3426.323 kb on - strand, within recJ at 3426.371 kb on + strand, within recJ at 3426.371 kb on + strand, within recJ at 3426.371 kb on + strand, within recJ at 3426.371 kb on + strand, within recJ at 3426.371 kb on + strand, within recJ at 3426.372 kb on - strand, within recJ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 3,423,769 + -3.2 3,423,770 - +0.7 3,423,770 - +1.4 3,423,770 - -1.4 3,423,784 - -0.7 3,423,798 - +0.2 3,423,804 + +1.1 3,423,804 + -0.0 3,423,826 + -0.0 3,423,826 + +0.6 3,423,826 + -0.4 3,424,248 + xerD NIAGMN_17335 0.46 -0.2 3,424,265 - xerD NIAGMN_17335 0.48 -0.5 3,424,638 - xerD NIAGMN_17335 0.89 -2.1 3,424,758 + -0.1 3,424,773 + +0.2 3,424,773 + +0.4 3,424,773 + -0.4 3,424,773 + -0.1 3,424,773 + +0.6 3,424,774 - +0.2 3,424,774 - +0.6 3,424,774 - -0.4 3,424,782 + +1.1 3,424,783 - -0.3 3,424,889 + dsbC NIAGMN_17340 0.18 -0.5 3,424,914 + dsbC NIAGMN_17340 0.22 +0.6 3,424,930 + dsbC NIAGMN_17340 0.24 -1.5 3,424,930 + dsbC NIAGMN_17340 0.24 +0.4 3,424,930 + dsbC NIAGMN_17340 0.24 +0.9 3,424,930 + dsbC NIAGMN_17340 0.24 -0.2 3,424,931 - dsbC NIAGMN_17340 0.24 -0.2 3,424,947 + dsbC NIAGMN_17340 0.26 +1.0 3,424,947 + dsbC NIAGMN_17340 0.26 +0.8 3,424,947 + dsbC NIAGMN_17340 0.26 -1.8 3,424,947 + dsbC NIAGMN_17340 0.26 -0.1 3,424,953 + dsbC NIAGMN_17340 0.27 +0.4 3,424,953 + dsbC NIAGMN_17340 0.27 +0.2 3,424,953 + dsbC NIAGMN_17340 0.27 +0.5 3,424,953 + dsbC NIAGMN_17340 0.27 +0.5 3,424,953 + dsbC NIAGMN_17340 0.27 -1.5 3,424,953 + dsbC NIAGMN_17340 0.27 +0.3 3,424,953 + dsbC NIAGMN_17340 0.27 +0.8 3,424,954 - dsbC NIAGMN_17340 0.27 -0.4 3,424,954 - dsbC NIAGMN_17340 0.27 -1.1 3,424,954 - dsbC NIAGMN_17340 0.27 +0.3 3,424,954 - dsbC NIAGMN_17340 0.27 +0.5 3,424,954 - dsbC NIAGMN_17340 0.27 -0.2 3,424,972 + dsbC NIAGMN_17340 0.30 +0.1 3,424,972 + dsbC NIAGMN_17340 0.30 +0.3 3,424,972 + dsbC NIAGMN_17340 0.30 +0.8 3,424,972 + dsbC NIAGMN_17340 0.30 +0.3 3,424,972 + dsbC NIAGMN_17340 0.30 -0.2 3,424,972 + dsbC NIAGMN_17340 0.30 +0.5 3,424,972 + dsbC NIAGMN_17340 0.30 -1.8 3,424,973 - dsbC NIAGMN_17340 0.30 -0.4 3,424,973 - dsbC NIAGMN_17340 0.30 -0.4 3,424,973 - dsbC NIAGMN_17340 0.30 +0.6 3,424,973 - dsbC NIAGMN_17340 0.30 -1.1 3,425,010 + dsbC NIAGMN_17340 0.35 -1.0 3,425,010 + dsbC NIAGMN_17340 0.35 +1.6 3,425,010 + dsbC NIAGMN_17340 0.35 +1.0 3,425,010 + dsbC NIAGMN_17340 0.35 +0.3 3,425,011 - dsbC NIAGMN_17340 0.35 -0.8 3,425,021 + dsbC NIAGMN_17340 0.37 -0.2 3,425,022 - dsbC NIAGMN_17340 0.37 +1.3 3,425,062 - dsbC NIAGMN_17340 0.43 +0.0 3,425,097 + dsbC NIAGMN_17340 0.48 -1.2 3,425,097 + dsbC NIAGMN_17340 0.48 -0.2 3,425,103 + dsbC NIAGMN_17340 0.48 +0.9 3,425,104 - dsbC NIAGMN_17340 0.49 -1.0 3,425,104 - dsbC NIAGMN_17340 0.49 -0.6 3,425,106 + dsbC NIAGMN_17340 0.49 -0.3 3,425,106 + dsbC NIAGMN_17340 0.49 -0.5 3,425,106 + dsbC NIAGMN_17340 0.49 +0.1 3,425,106 + dsbC NIAGMN_17340 0.49 -1.4 3,425,107 - dsbC NIAGMN_17340 0.49 +1.5 3,425,149 + dsbC NIAGMN_17340 0.55 +0.3 3,425,149 + dsbC NIAGMN_17340 0.55 +0.1 3,425,149 + dsbC NIAGMN_17340 0.55 -0.4 3,425,149 + dsbC NIAGMN_17340 0.55 +0.0 3,425,149 + dsbC NIAGMN_17340 0.55 -1.3 3,425,149 + dsbC NIAGMN_17340 0.55 -1.1 3,425,149 + dsbC NIAGMN_17340 0.55 -1.3 3,425,150 - dsbC NIAGMN_17340 0.55 -0.5 3,425,150 - dsbC NIAGMN_17340 0.55 -2.8 3,425,150 - dsbC NIAGMN_17340 0.55 +0.1 3,425,150 - dsbC NIAGMN_17340 0.55 -1.4 3,425,151 + dsbC NIAGMN_17340 0.55 -0.4 3,425,151 + dsbC NIAGMN_17340 0.55 -1.3 3,425,177 - dsbC NIAGMN_17340 0.59 -1.9 3,425,232 + dsbC NIAGMN_17340 0.67 -0.1 3,425,284 - dsbC NIAGMN_17340 0.74 +1.0 3,425,316 + dsbC NIAGMN_17340 0.78 -0.1 3,425,329 + dsbC NIAGMN_17340 0.80 +0.6 3,425,467 + +0.2 3,425,468 - +0.0 3,425,488 + +0.4 3,425,488 + +0.2 3,425,489 - -0.3 3,425,489 - +1.1 3,425,489 - -0.0 3,425,489 - -0.7 3,425,561 - -0.4 3,425,562 + +2.0 3,425,578 + -0.0 3,425,578 + -0.1 3,425,579 - +0.6 3,425,615 + -0.2 3,425,615 + +2.6 3,425,667 + recJ NIAGMN_17345 0.11 +0.2 3,425,667 + recJ NIAGMN_17345 0.11 -0.4 3,425,667 + recJ NIAGMN_17345 0.11 -0.7 3,425,668 - recJ NIAGMN_17345 0.11 +1.6 3,425,668 - recJ NIAGMN_17345 0.11 -0.3 3,425,696 + recJ NIAGMN_17345 0.13 +0.5 3,425,697 - recJ NIAGMN_17345 0.13 +1.6 3,425,697 - recJ NIAGMN_17345 0.13 +0.9 3,425,743 + recJ NIAGMN_17345 0.15 -0.5 3,425,743 + recJ NIAGMN_17345 0.15 +0.3 3,425,869 + recJ NIAGMN_17345 0.23 +0.2 3,425,869 + recJ NIAGMN_17345 0.23 -0.3 3,425,891 + recJ NIAGMN_17345 0.24 +0.3 3,425,891 + recJ NIAGMN_17345 0.24 -0.2 3,425,892 - recJ NIAGMN_17345 0.24 -0.2 3,426,006 - recJ NIAGMN_17345 0.31 +1.9 3,426,063 + recJ NIAGMN_17345 0.34 -2.9 3,426,063 + recJ NIAGMN_17345 0.34 -1.1 3,426,063 + recJ NIAGMN_17345 0.34 +1.0 3,426,064 - recJ NIAGMN_17345 0.34 -0.3 3,426,064 - recJ NIAGMN_17345 0.34 -2.2 3,426,134 + recJ NIAGMN_17345 0.38 -1.0 3,426,201 - recJ NIAGMN_17345 0.42 -1.6 3,426,201 - recJ NIAGMN_17345 0.42 -1.1 3,426,203 + recJ NIAGMN_17345 0.42 +0.5 3,426,322 + recJ NIAGMN_17345 0.49 -1.1 3,426,323 - recJ NIAGMN_17345 0.49 +0.1 3,426,371 + recJ NIAGMN_17345 0.52 -0.2 3,426,371 + recJ NIAGMN_17345 0.52 +0.6 3,426,371 + recJ NIAGMN_17345 0.52 -1.2 3,426,371 + recJ NIAGMN_17345 0.52 +0.5 3,426,371 + recJ NIAGMN_17345 0.52 +0.2 3,426,372 - recJ NIAGMN_17345 0.52 +1.0
Or see this region's nucleotide sequence